Synthesis of Biodegradable Scaffold from Nano Fibers and Evaluation of Protein Adsorption Behaviors on its Surface

Abstract

The effects of various processing parameters of electro spinning such as concentration, applied voltage, distance from needle to collector on the morphology(especially fiber diameter) of nanofiber matrix was investigated in this study. We found out the concentration of polymer solution was the key parameter to control the fiber diameter. Such a difference on surface morphology of electro spun nanofiber matrix under various processing parameters will show the different behaviors on protein adsorption on the surface contacting with body fluid. Consequently, this results different cell motions on the matrix used on tissue engineering. To evaluate the adsorption of proteins on the surface of sheet type nanofiber matrix, matrix obtained by electro spinning were immersed in FITC labeled proteins solutions. And then, we confirmed adsorption of proteins using laser scanning confocal microscopy (LSCM). The quantitative analysis of adsorbed proteins was also investigated by UV spectroscopy.

Promising New Horizons in Medicine: Medical Advancements with Nanocomposite Manufacturing via 3D Printing

“…Polymers and the corresponding composites are growing increasingly popular in the healthcare sector as an outcome of their distinguishing thermal, mechanical, electrical, and spectral characteristics. This is especially helpful when it is impossible to precisely match the unique anatomical structure of each patient using traditional manufacturing techniques [37,38].…”