Synthesis of ∈-aspartyl-lysines and of isohexylamides of aspartic acid

Formation of ∈-(aminosuccinyl)-lysine from ∈-aspartyl-lysine from bacitracin A, and from the cell walls of lactobacilli

“…However, both synthetic peptides yield a derivative of aminosuccinimide, E-(aminosuccinyl)-lysine (I), which appears to be identical with the product from the antibiotic, on treatment with concentrated hydrochloric acid at 80°. A similar type of cycization has been found to occur with the m-and l-.i8ohexylamides of aspartic acid (Swallow et al 1958) and (less readily) with aX-(Ox-Laspartyl) -Llysine and a -(p -Laspartyl) -Llysine which have been synthesized by Pimlott & Young (unpublished work).…”

“…Experiments recorded in this paper provide an explanation of the anomalous behaviour of the products containing lysine and aspartic acid which are formed from bacitracin A. Synthetic e-(Q-Laspartyl) -L-lysine and e -(f-L-aspartyl) -L-lysine (Swallow, Lockhart & Abraham, 1958) are both different from the 'Asp. Lys' found in hydrolysates of bacitracin A.…”

“…Material8 e-A8partyl-lysine8. The aand ,-isomers were prepared as described by Swallow et al (1958). cx-Aspartyl-ly8ine8. The two isomers were kindly provided by Miss P. Pimlott and Dr G. T. Young.…”

“…Chromatography and electrophoresis on Whatman no. 1 paper were carried out in the systems described by Swallow et al (1958). In general, 20-40jig.…”

Nucleoside Phosphotransferase from Malt Sprouts. II. Studies on the Active Site and the Phospho-Intermediate

“…Fig. 5 shows a Scatchard plot^1 6 the protein for the inhibitor can be derived. The dissociation constant of the nucleotide (K a = 1.2 χ 10~5M), which can be calculated from the slope of the plot, is in good agreement with the above mentioned K-t .…”