Synthesis of anisotropic silver nanoparticles using silk fibroin: characterization and antimicrobial properties

Sangappa, Y.; Latha, S.; Asha, S.; Sindhu, P; Parushuram, N.; Shilpa, M.; Byrappa, K.; Narayana, B.

doi:10.1080/14328917.2017.1383680

Cited by 27 publications

(12 citation statements)

References 31 publications

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“…Therefore, the obtained results confirmed the differential reduction pattern of silver by various peptone components. A similar reduction of silver by various components yielded to the anisotropic silver nano-particles were obtained by the previous reports [ 30 , 31 ]. Though the monodisperse nature of silver nanoparticles is the main issue associated with synthesis, the green-route synthesized anisotropic silver nanoparticles are known to have a great advantage in biological platforms, as the silver nanoparticles interact with microorganisms in a shape-dependent manner [ 30 , 32 ].…”

Section: Resultssupporting

confidence: 82%

“…However, the obtained results suggest that the Pep-AgNPs are potent anti-bacterial materials for the gram-positive, as well as the gram-negative, bacteria. Anisotropic silver nanoparticles of silk fibroin gave anti-bacterial activity only towards the gram-negative bacteria, and gram-positive bacteria was found to be resistant [ 31 ]. However, the anisotropic Pep-AgNPs exhibited anti-bacterial activity towards both gram-positive and gram-negative bacteria, which might be due to the bacteria getting attracted towards the nutrient peptone and increased interactions with the Pep-AgNPs.…”

Section: Resultsmentioning

confidence: 99%

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Green-Synthesis of Anisotropic Peptone-Silver Nanoparticles and Its Potential Application as Anti-Bacterial Agent

et al. 2019

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This study demonstrates a green-route-based synthesis of high-concentration suspensions of anisotropic silver nanoparticles (AgNPs) by peptone (Pep), a soluble protein hydrolysate and an abundantly used nutrient source in microbial-media. The transformation of Ag ions from solution into a high-concentration suspension of anisotropic Pep-AgNPs, at an extremely low concentration of peptone (0.02%), indicates that the present green-route synthesis method follows “low volume high concentration nano-synthesis”, and, hence, enhances the economic significance of the process. Process optimization with different concentrations of AgNPs (1–5 mM), NaOH solution (5–40 mM), and peptone (0.004%–0.12%) gave the optimized Pep-AgNPs synthesis at 3 mM of AgNO3, 20 mM of NaOH, and 0.02% of the peptone concentrations. The green-route synthesized Pep-AgNPs were structurally characterized by the TEM, XPS, FT-IR, and XRD analyses. The Pep-AgNPs against the clinically relevant bacteria Escherichia coli and Staphylococcus aureus gave significant anti-bacterial properties, with a MIC (minimum inhibitory concentration) of 100 ppm. The colony counting and morphological observation of the bacterial cell under SEM corroborated an anti-bacterial potential of the Pep-AgNPs. Therefore, Pep-AgNPs are green-route synthesized, anisotropic, and have a significant anti-bacterial potential that can be used in many relevant applications.

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Section: Resultssupporting

confidence: 82%

Section: Resultsmentioning

confidence: 99%

Green-Synthesis of Anisotropic Peptone-Silver Nanoparticles and Its Potential Application as Anti-Bacterial Agent

et al. 2019

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“…However, previous reports show anisotropic AgNPs also have a good antimicrobial effect [ 30 , 31 ]. Moreover, many reports display that the antimicrobial activity of anisotropic AgNPs as being higher than silver nanospheres [ 32 ]. Anisotropic AgNPs, such as truncated triangular, display the strongest antibacterial action, compared with spheres [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

Anisotropic Silver Nanoparticles Gel Exhibits Antibacterial Action and Reduced Scar Formation on Wounds Contaminated with Methicillin-Resistant Staphylococcus pseudintermedius (MRSP) in a Mice Model

Thammawithan

Srichaiyapol

Siritongsuk

et al. 2021

Animals

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Staphylococcus pseudintermedius (S. pseudintermedius) infected wounds can cause seriously delayed wound healing processes in animals. Antimicrobial agents that have antimicrobial and wound healing efficacy have become an essential tool for overcoming this problem. In our previous study, anisotropic AgNPs have been reported to have antimicrobial efficiency against animal and human pathogens, and could be suitable as antimicrobial agents for infected wounds. Here, antimicrobial and wound healing activities of anisotropic AgNPs gels were assessed in vivo. BALB/cAJcl mice wounds were infected by Methicillin-resistant Staphylococcus pseudintermedius (MRSP). Then, antibacterial and wound healing activities were evaluated by bacterial cell count, wound contraction, digital capture, and histology. The results show that anisotropic AgNPs gels could eliminate all bacterial cell infected wounds within 7 days, the same as povidone iodine. Wound healing activity was evaluated by wound contraction (%). The results showed 100% wound contraction in groups treated with anisotropic AgNPs gels within 14 days that was not significantly different from povidone iodine and control gel without AgNPs. However, the digital capture of wounds on day 4 showed that anisotropic AgNPs gel prevented pus formation and reduced scar appearance within 21 days. The histology results exhibit improved collagen fiber alignment that supports scar disappearance. In conclusion, these results indicate that anisotropic AgNPs gels are suitable for treating infected wounds. The gel is effective in eliminating bacteria that supports the natural process of wound repair and also causes reduced scar formation.

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“…Studies have investigated the antibacterial efficiency of anisotropic AgNPs (e.g., triangular prisms, hexagons, truncated triangles, etc.) and found that they had a higher antimicrobial properties than AgNSs [ 23 , 24 ]. The antimicrobial activity of AgNSs and anisotropic AgNPs was evaluated depending on the physical properties, especially size and shape [ 37 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

“…For application, anisotropic AgNPs have been used to colour wool fabrics and show high antibacterial activity against E. coli [ 22 ]. Some studies have demonstrated that anisotropic AgNPs have significantantibacterial activity against tested human pathogens compared with AgNSs [ 23 ]. In addition, Sukdeb Pal et al, 2007 have reported truncated triangular Ag nanoplates with a {111} plan showed the strongest biocidal action compared with spherical and rod-shaped nanoparticles [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

A Biological Study of Anisotropic Silver Nanoparticles and Their Antimicrobial Application for Topical Use

Thammawithan

Siritongsuk

Nasompag

et al. 2021

Veterinary Sciences

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The excessive use of antibiotics in both human and veterinary medicine has contributed to the development and rapid spread of drug resistance in bacteria. Silver nanoparticles (AgNPs) have become a tool of choice that can be used to treat these resistant bacteria. Several studies have shown that AgNPs have antibacterial and wound healing properties. In this study, we evaluated the biological activity of anisotropic AgNPs to develop an antimicrobial gel formulation for treating wound infections. We showed that some anisotropic AgNPs (S2) have an effective antibacterial activity against bacterial pathogens and low cytotoxicity to keratinocytes and fibroblasts in vitro. The MIC and MBC values were in the range of 2–32 µg/mL, and cytotoxicity had IC50 values of 68.20 ± 9.71 µg/mL and 68.65 ± 10.97 µg/mL against human keratinocyte and normal human dermal fibroblast cells, respectively. The anisotropic AgNPs (S2) were used as a gel component and tested for antibacterial activity, including long-term protection, compared with povidone iodine, a common antiseptic agent. The results show that the anisotropic AgNPs can inhibit the growth of most tested bacterial pathogens and provide protection longer than 48 h, whereas povidone iodine only inhibits the growth of some bacteria. This study suggests that anisotropic AgNPs could be used as an alternative antimicrobial agent for treating bacterial skin infection and as a wound healing formulation.

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Synthesis of anisotropic silver nanoparticles using silk fibroin: characterization and antimicrobial properties

Cited by 27 publications

References 31 publications

Green-Synthesis of Anisotropic Peptone-Silver Nanoparticles and Its Potential Application as Anti-Bacterial Agent

Green-Synthesis of Anisotropic Peptone-Silver Nanoparticles and Its Potential Application as Anti-Bacterial Agent

Anisotropic Silver Nanoparticles Gel Exhibits Antibacterial Action and Reduced Scar Formation on Wounds Contaminated with Methicillin-Resistant Staphylococcus pseudintermedius (MRSP) in a Mice Model

A Biological Study of Anisotropic Silver Nanoparticles and Their Antimicrobial Application for Topical Use

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