Synthesis of Acanthus ilicifolius Linn alkaloid 2-Benzoxazolinone Derivative and its effect on cervical cancer C-33A cells

Abstract

Cervical cancer seriously threatens women’s health, it seriously harm the patient’s physical and mental health. The new derivative of Acanthus ilicifolius Linn alkaloid 2-benzoxazone was obtained from o-aminophenol, substituted benzaldehyde, trichloroacetic acid and isocyanate via a tandem Ugi 4CC/SN cyclization (N-cyclohexyl-2-(2-benzoxazolone-3-yl)-2-p-trifluoromethylphenylacetamide, BOABB), and the toxicity of the new compound to the cervical cancer C-33A cells was detected by CCK-8 assay, wound healing assay and apoptotic assay. The results exhibited that BOABB had obvious inhibitory effect on C-33A cells, and the calculated IC50 was 32.3 μM. Wound healing assay showed that BOABB could significantly inhibit cell migration (P<0.05). The apoptotic assay demonstrated that BOABB has induced apoptosis in C-33A cells (from 10.86% to 34.70%).

HPLC Determination of Benzoxazolinone on Jeruju Plant (Acanthus Ilicifolius): Narrative Review

“…On research Tian et al (2021) using the leaves of the jeruju plant (Acanthus ilicifolius) to determine the inhibitory effect of BOABB compounds on the proliferation and apoptosis of C-33A cervical cancer cells with the NMR analysis method and using the CCK-8 test. It was found that BOABB had a pronounced inhibitory effect on C-33A cells, with an IC50 of 32.3 M. Apoptosis assays showed that BOABB had induced apoptosis in C-33A cells (from 10.86% to 34.70%).…”

“…Based on Table 1, most experts choose analysis methods using Nuclear Magnetic Resonance (NMR) Spectroscopy, among others, namely (Bhattarai et al, 2022;Kanchanapoom et al, 2001;Tian et al, 2021;Zhao et al, 2015) Nuclear Magnetic Resonance (NMR) Spectroscopy is a spectroscopic method that is very useful in determining the structure of a chemical compound, both proton (1H) and carbon (13C) NMR. This method is based on the magnetic moment of the atomic nucleus.…”

“…Most of the experts shown in Table 1 use the leaf part in the jeruju plant (Acanthus ilicifolius) which is used as extraction, among others. (Kanchanapoom et al, 2001;Tian et al, 2021;Yin et al, 2008) Jeruju leaves can be used for energy recovery after giving birth by boiling and used for bathing water, restoring stamina after giving birth and preventing infection in the uterus. In addition, jeruju leaf extract contains alkaloids compounds, flavonoids, polyphenols, phenyletanoid glycosides and coumarins.…”

2-Benzoxazolinone as Breast Cancer Cells Inhibitor via Estrogen Receptor

“…The leaves of Acanthus ilicifolius contains steroid, triterpenoid, flavonoid, tannin, saponin, and alkaloid (12), including α-Amyrin, Acanthicifoline, quercetin, and 2-BOA (13). Acanthus ilicifolius extracts and fractions are reported to have activity to inhibit the proliferation of various cancer cell lines (11,(13)(14)(15)(16)(17). However, none of the reports discover the mechanism of inhibition related to ER.…”