Synthesis of a Re-usable Cellobiase Enzyme Catalyst through In situ Encapsulation in Nonsurfactant Templated Sol–Gel Mesoporous Silica

Wei, Yen; Das, Sudipto; Berke-Schlessel, David; Ji, Hai‐Feng; McDonough, John K.; Feng, Lin; Zhang, Xiang; Zhai, Wentao; Cao, Ya

doi:10.1007/s11244-012-9892-1

Cited by 5 publications

(8 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Possibly, the presence of them affected of growth of the particles during the polycondensation process, which was reflected on BET area, pore volume, pore diameter and the mechanical stability of biocatalysts. Similar effects have been reported in silicas synthesized by using surfactants or sugars as templates [21,27].…”

Section: Effect Of the Presence Of Sugars On Siliceous Matrix Propertiessupporting

confidence: 84%

“…The glucose, fructose and sucrose were selected for this study due to positive effect reported on the enzyme activity/stability for other enzymes and on the silica polycondensation .…”

Section: Resultsmentioning

confidence: 99%

“…The glucose, fructose and sucrose were selected for this study due to positive effect reported on the enzyme activity/stability for other enzymes [20] and on the silica polycondensation [21]. The G-24-BS, S-24-BS and F-24-BS biocatalysts prepared in the presence of glucose, sucrose and fructose during 24 h, respectively, exhibit a higher EA and immobilization yield than 24-BS obtained in absence of sugar for 24 h (Table 1).…”

Section: Effect Of the Presence Of Sugar On Preparation Of β-Gal/silimentioning

confidence: 99%

“…However, this quantity criterion is not unique for explaining the activity, since the higher amount of EP for 24-BS biocatalyst does not give the higher EA. This highlights the importance of the fructose in the encapsulating process because it also protects the enzyme against deactivation [20,21].…”

Section: Effect Of the Presence Of Sugars On Siliceous Matrix Propertiesmentioning

confidence: 99%

“…The enzyme can be protected by using additives that stabilize it, such as surfactants and sugars , which also are template for small pores. Even sugars such as fructose have been used as templates for obtaining mesoporous silica , its use as stabilizing agents during silica sol‐gel encapsulation of enzymes has not been reported to date.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

In situ immobilization of β‐galactosidase from Bacillus circulans in silica by sol‐gel process: Application in prebiotic synthesis

Escobar

Illanes

Wilson

et al. 2016

Engineering in Life Sciences

View full text Add to dashboard Cite

In situ immobilization of β-galactosidase from Bacillus circulans in silica by sol-gel process: Application in prebiotic synthesisThe enzyme encapsulation is a very well-known stabilization pathway. However, there are some challenges in order to avoid the enzyme denaturation under encapsulation conditions. The β-galactosidase from Bacillus circulans was immobilized through sol-gel encapsulation route assisted by Triton X-100 surfactant and sugars. The effects of sugar presence in the immobilization process and the gelation time on the biocatalyst activity/stability were explained taking into account the characteristics of the formed silica matrix and the changes of the enzyme environment. The enzyme was effectively immobilized by this strategy, with high immobilization yield in terms of activity (29%) and expressed activity (47 IU/g). The immobilization through silica sol-gel in the presence of 1×10 −3 M Triton X-100 and fructose conferred 28.4-fold higher stability to the enzyme compared with the soluble form. This is an advantage for its use in the synthesis of the galacto-oligosaccharides at 50˚C. The total lactose conversion to galacto-oligosaccharides was 26%wt, which is comparable with that reported in the literature. The obtained biocatalyst is useful for the synthesis of galacto-oligosaccharides and its catalytic behavior is rationalized in this work. IntroductionThe β-galactosidase from Bacillus circulans is a hydrolase (E.C.

show abstract

Section: Effect Of the Presence Of Sugars On Siliceous Matrix Propertiessupporting

confidence: 84%

“…The glucose, fructose and sucrose were selected for this study due to positive effect reported on the enzyme activity/stability for other enzymes and on the silica polycondensation .…”

Section: Resultsmentioning

confidence: 99%

Section: Effect Of the Presence Of Sugar On Preparation Of β-Gal/silimentioning

confidence: 99%

Section: Effect Of the Presence Of Sugars On Siliceous Matrix Propertiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

In situ immobilization of β‐galactosidase from Bacillus circulans in silica by sol‐gel process: Application in prebiotic synthesis

Escobar

Illanes

Wilson

et al. 2016

Engineering in Life Sciences

View full text Add to dashboard Cite

show abstract

A Microfluidic Countercurrent Reactor for Accelerating Enzymatic Reactions

et al. 2023

View full text Add to dashboard Cite

Enzymes are important catalysts in biochemical reactions with superior regio, stereo, and substrate selectivity. However, enzymatic reaction systems have drawbacks including product inhibition, difficulty recycling, and poor stability. Importantly, the rate of an enzyme catalyzed reaction diminishes rapidly due to product inhibition and substrate depletion, making it difficult for many enzymes to catalyze a reaction to completion. The outcome is a mixture of unreacted substrates being present in the final reaction, necessitating additional separation steps that increase costs. This study presents a microfluidic reactor for accelerating enzyme catalyzed reactions using a countercurrent design that continuously removes products and adds fresh substrate into the reaction, allowing enzymes to operate under better reaction conditions. It demonstrates that countercurrent flow accelerates enzymatic reactions in our system up to 36 % for horseradish peroxidase and 21 % for β‐glucosidase compared to cocurrent flow, and the resulting reaction solution contains highly pure product with minimal substrate contamination.

show abstract

Understanding the silica-based sol-gel encapsulation mechanism of Thermomyces lanuginosus lipase: The role of polyethylenimine

Escobar

Bernal

Bolívar

et al. 2018

Molecular Catalysis

View full text Add to dashboard Cite

Synthesis of a Re-usable Cellobiase Enzyme Catalyst through In situ Encapsulation in Nonsurfactant Templated Sol–Gel Mesoporous Silica

Cited by 5 publications

References 38 publications

In situ immobilization of β‐galactosidase from Bacillus circulans in silica by sol‐gel process: Application in prebiotic synthesis

In situ immobilization of β‐galactosidase from Bacillus circulans in silica by sol‐gel process: Application in prebiotic synthesis

A Microfluidic Countercurrent Reactor for Accelerating Enzymatic Reactions

Understanding the silica-based sol-gel encapsulation mechanism of Thermomyces lanuginosus lipase: The role of polyethylenimine

Contact Info

Product

Resources

About