Understanding the silica-based sol-gel encapsulation mechanism of Thermomyces lanuginosus lipase: The role of polyethylenimine

Enzyme entrapment, biocatalyst immobilization without covalent attachment

“…Adding polyethylenimine to a silica gel of lipase from Thermomyces lanuginosus immobilized by a TEOS sol-gel process increased the enzyme loading, thermal stability and catalytic activity. 114 Similarly adding poly-L-lysine to silica gels of lipase and glucose oxidase enhanced the stability, reusability and thermal stability. 115,116 Adding polyethylene oxide to a TEOS derived silica gel of β-glucuronidase from Helix pomatia enabled the microfluidic (the reaction was operated in small quantities of liquids within narrow channels) hydrolysis of p-nitrophenyl-β-D-glucuronide ( pNP-GluA).…”

Enzymatic synthesis of 4-methoxycinnamoylglycerol Uv filter mediated by immobilized-lipase and nanoparticle formation on N-succinylchitosan

“…The catalytic activity of the immobilized-lipase biocatalyst was measured by p-NPB UV/Vis activity assay (Escobar et al 2018) before and after its use in the 4-MCG synthesis. The preserved (residual) activity was reported as percentage respecting to the initial activity.…”

“…Its immobilization has improved its catalytic behavior in this kind of reaction due to the interfacial activation (Bassi et al 2016;Zhou et al 2012), thermal and conformational stabilization (Bernal et al 2018;Matte et al 2014) conferred by the support. Moreover, its immobilization by one-pot silica sol-gel process in the presence of polyethilenimine (PEI) is so simple for obtaining a biocatalyst with high protein load (90%), encapsulation yield (47%) and 247-times more stable at 65°C than the soluble enzyme (Escobar et al 2018), which could increase the conversion degree of 4-MCA and glycerol during the esterification reaction at 65ºC. This immobilized-lipase has been not tested yet for production of 4-MCG.…”

Application of Immobilized Enzymes in Food Industry

“…It also improves biocatalytic behavior of immobilized enzymes and increases their thermal stability and recovered activity. 165 ■ ENZYME IMMOBILIZATION EFFICIENCY ON…”