Synthesis of a ratiometric fluorescent peptide sensor for the highly selective detection of Cd2+

Li, Yan; Li, Lianzhi; Pu, Xuewei; Ma, Guolin; Wang, Erqiong; Kong, Jinming; Liu, Zhipeng; Liu, Yangzhong

doi:10.1016/j.bmcl.2012.04.088

Cited by 61 publications

(24 citation statements)

References 43 publications

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“…As shown in Figure , when excited at 290 nm wavelength, with the addition of increasing Hg 2+ concentrations, fluorescence intensity decreased at 360 nm and increased at 500 nm. Fluorescence emission at 360 nm is a characteristic of Trp residues, whereas fluorescence emission at 500 nm is attributed to dansyl chromophores . When Hg 2+ was added to the D‐P5 solution, amino acid residues in D‐P5 (including imidazole group of histidine and indole group of tryptophan) can interact with Hg 2+ , therefore the peptide may fold and the distance between the dansyl fluorophore (acceptor) and Trp residue (donor) would be shortened, resulting in a decrease in emission intensity for Trp (360 nm) and an increase in emission intensity for the dansyl group (500 nm) due to the fluorescence resonance energy transfer (FRET) effect .…”

Section: Resultsmentioning

confidence: 99%

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Multifunctional peptide‐based fluorescent chemosensor for detection of Hg²⁺, Cu²⁺ and S²⁻ ions

et al. 2019

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A novel multifunctional fluorescent peptide sensor based on pentapeptide dansyl-Gly-His-Gly-Gly-Trp-COOH (D-P5) was designed and synthesized efficiently using Fmoc solid-phase peptide synthesis (SPPS). This fluorescent peptide sensor shows selective and sensitive responses to Hg 2+ and Cu 2+ among 17 metal ions and six anions studied in N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES) buffer solution. The peptide probe differentiates Hg 2+ and Cu 2+ ions by a 'turn-on' response to Hg 2+ and a 'turn-off' response to Cu 2+ . Upon addition of Hg 2+ or Cu 2+ ions, the sensor displayed an apparent color change that was visible under an ultraviolet lamp to the naked eye. The limits of detection (LOD) of DP-5 were 25.0 nM for Hg 2+ and 85.0 nM for Cu 2+ ; the detection limits for Cu 2+ were much lower than the drinking water maximum contaminant levels set out by the United States Environmental Protection Agency (USEPA). It is noteworthy that both D-P5-Hg and D-P5-Cu systems were also used to detect S 2− successfully based on the formation of ternary complexes. The LODs of D-P5-Hg and D-P5-Cu systems for S 2− were 217.0 nM and 380.0 nM, respectively. Furthermore, the binding stoichiometry, binding affinity and pH sensitivity of the probe for Hg 2+ and Cu 2+ were investigated. This study gives new possibilities for using a short fluorescent peptide sensor for multifunctional detection, especially for anions. KEYWORDS cupric ion, fluorescent sensor, mercury ion, peptide-based sensor, sulfide ion

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Section: Resultsmentioning

confidence: 99%

“…Fluorescent chemical probes are a useful tool for detecting low concentrations of HTM ions, which are significant in living organisms and the environment . Fluorescent chemosensors can be used as portable and less costly alternatives to traditional atomic spectrometric techniques .…”

Section: Introductionmentioning

confidence: 99%

Multifunctional peptide‐based fluorescent chemosensor for detection of Hg²⁺, Cu²⁺ and S²⁻ ions

et al. 2019

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“…Dans-CPGCW-NH 2 . 40 A szerzõk különbözõ fémionok hatására jelentõs fluoreszcencia intenzitás-növekedést mutattak ki a danzilcsoportra jellemzõ hullámhossz-tartományban (=500-550 nm között) függetlenül attól, hogy a danzilcsoport vagy a triptofán abszorpciós sávján gerjesztették a rendszereket. Utóbbi esetben az intenzitás növekedését a szerzõk a fémionok által indukált FRET jelenségként azonosították.…”

Section: Az Afarsr Fehérje As(iii)-kötõ Szakaszát Modellezõ Peptid VIunclassified

Metalloproteinek fémkötőhelyein alapuló oligopeptidek, mint potenciális toxikus fémion érzékelők

Szekeres¹,

Szunyogh²,

Galbács³

et al. 2017

MKF

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“…The developed fluorescent probes were mainly based on crown ether- 24,25 and anthracene-based 26,27 structures, but proprietary aromatic structures, including dipyridyl-phenantroline, 28 as well as naphthyridine-based 29 and tricarbocyanine-based molecules 30 were also described. There are only a very few examples for fluorescent Cd(II)-sensing synthetic peptides 18,31,32 but none of these probes were immobilized on a solid support. Limit of detection (LOD) values are scarcely reported in the literature for the Cd(II) fluorescent probes, most probably due to the fact that LODs would be not only influenced by the probe but also largely by the sensor construction itself.…”

Section: Introductionmentioning

confidence: 99%

“…Limit of detection (LOD) values are scarcely reported in the literature for the Cd(II) fluorescent probes, most probably due to the fact that LODs would be not only influenced by the probe but also largely by the sensor construction itself. The published values range from nM to M 18,23,32 (for two reported peptidyl probes: 41 nM 18 and 294 nM 32 ).…”

Section: Introductionmentioning

confidence: 99%

Cd(II) Capture Ability of an Immobilized, Fluorescent Hexapeptide

Galbács

Szokolai

Kormányos

et al. 2015

Bulletin of the Chemical Society of Japan

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Cys-Tyr-(YY), targeting Cd(II) ion sensing, was synthesized on various solid supports including two resins, as well as glass and quartz surfaces. The synthesis was based on the Fmoc (9-fluorenylmethoxycarbonyl) and the APTES (3-aminopropyltriethoxysilane) methodologies on the resin and silica supports, respectively. The immobilized ligand, except when coupled to a hydrophobic benzhydrylamine resin, showed a remarkably efficient, pHdependent Cd(II) capturing ability with a maximum binding capacity around neutral pH. The effect of contact time and metal ion concentration was also studied with a hydrophilic resin supported peptide (YY-NTG). The interaction of YY-NTG with Cd(II) was investigated by pH-potentiometric titrations in aqueous samples containing the resin beads and Cd(II). These studies, together with metal ion capturing experiments under buffer-controlled pH, prove that each immobilized peptide can bind one Cd(II) ion at pH=7.0 in the presence of one equivalent metal ion or metal ion excess. For Cd(II) binding to YY-NTG a notably high, K=1.310 10 apparent stability constant was determined (1:1 metal-to-ligand ratio, pH=7.0). Analytical results suggests that the concentration of Cd(II) can be measured below 200 nM with this silica-supported peptide. The usefulness of the probe was demonstrated by fluorescence spectroscopy.

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Synthesis of a ratiometric fluorescent peptide sensor for the highly selective detection of Cd2+

Cited by 61 publications

References 43 publications

Multifunctional peptide‐based fluorescent chemosensor for detection of Hg²⁺, Cu²⁺ and S²⁻ ions

Multifunctional peptide‐based fluorescent chemosensor for detection of Hg²⁺, Cu²⁺ and S²⁻ ions

Metalloproteinek fémkötőhelyein alapuló oligopeptidek, mint potenciális toxikus fémion érzékelők

Cd(II) Capture Ability of an Immobilized, Fluorescent Hexapeptide

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Synthesis of a ratiometric fluorescent peptide sensor for the highly selective detection of Cd2+

Cited by 61 publications

References 43 publications

Multifunctional peptide‐based fluorescent chemosensor for detection of Hg2+, Cu2+ and S2− ions

Multifunctional peptide‐based fluorescent chemosensor for detection of Hg2+, Cu2+ and S2− ions

Metalloproteinek fémkötőhelyein alapuló oligopeptidek, mint potenciális toxikus fémion érzékelők

Cd(II) Capture Ability of an Immobilized, Fluorescent Hexapeptide

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Product

Resources

About

Multifunctional peptide‐based fluorescent chemosensor for detection of Hg²⁺, Cu²⁺ and S²⁻ ions

Multifunctional peptide‐based fluorescent chemosensor for detection of Hg²⁺, Cu²⁺ and S²⁻ ions