Synthesis of a Deoxyribonucleic Acid Sequence Complementary to Ovalbumin Messenger Ribonucleic Acid and Quantification of Ovalbumin Genes

Sullivan, Drew; Palacios, Raphael; Stavnezer, Janet; Taylor, John M.; Faras, Anthony J.; Kiely, Mary L.; Summers, Nicholas; Bishop, J. Michael; Schimke, Robert T.

doi:10.1016/s0021-9258(19)43323-1

Cited by 146 publications

(13 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The hen oviduct offers an attractive model system for studying the regulation of gene expression by steroid hormones. Administration of estrogen to the newborn chick stimulates oviduct growth and differentiation and results in the appearance of a number of new specific intracellular proteins (O'Malley et al, 1969;Palmiter, 1972Palmiter, ,1973Chan etal., 1973;Harris et al, 1973Harris et al, , 1975Sullivan et al, 1973;O'Malley & Means, 1974;Hynes et al, 1977). The synthesis of one of these proteins, ovalbumin, has been studied extensively.…”

mentioning

confidence: 99%

Molecular cloning of ovomucoid gene sequences from partially purified ovomucoid messenger RNA

Stein¹,

Catterall²,

Woo³

et al. 1978

Biochemistry

View full text Add to dashboard Cite

mentioning

confidence: 99%

Molecular cloning of ovomucoid gene sequences from partially purified ovomucoid messenger RNA

Stein¹,

Catterall²,

Woo³

et al. 1978

Biochemistry

View full text Add to dashboard Cite

“…With ovalbumin mRNA, cDNA has been described which was heterogeneous in size with an average sedimentation coefficient of 5 S BIOCHEMISTRY, VOL. to 7 S (Harris et al, 1973;Cox et al, 1974;Sullivan et al, 1973) . It has been reported that the average size of the cDNA product can be increased by the presence of relatively high concentrations of dNTP's in the reaction mixture (Faust et al, 1973;Imaizumi et al, 1973).…”

mentioning

confidence: 99%

The synthesis and properties of the complete complementary DNA transcript of ovalbumin mRNA

Monahan¹,

Harris²,

Woo³

et al. 1976

Biochemistry

144

View full text Add to dashboard Cite

The synthesis of a complementary DNA copy (cDNA) of hen ovalbumin mRNA using AMV RNA-directed DNA polymerase was studied under different conditions of salt, deoxyribonucleotide concentrations, temperature, and time. It was observed that in the absence of monovalent cation at 46 degrees C a complete transcript of ovalbumin mRNA could be effected by the enzyme. The minimum deoxyribonucleotide requirement for complete synthesis was 35 muM for dATP, dGTP, and dCTP and 200 muM dTTP. By a number of different experimental criteria which included sedimentation on alkaline sucrose gradients and electrophoresis in polyacrylamide gels containing 98% formamide, direct electron microscope visualization, and protection of ovalbumin [25I]mRNA from nuclease digestion it could be demonstrated that a considerable fraction of a complete mRNA transcript was indeed synthesized. The cDNA/ovalbumin mRNA hybrid had a Tm on hydroxylapatite of 92 degrees C, indicating the synthesis of a RNA transcript with a high fidelity. When such a complete ovalbumin [3H]cDNA was synthesized with a specific activity of 10(8) cpm/mug and hyfridized to an excess of chick DNA, the kinetics of hybridization indicated that the cDNA was comprised of a nonrepetitive sequence.

show abstract

“…Reticulocytes from an adult anemic chicken were frozen in liquid nitrogen. DNA was isolated according to the procedure of Sullivan et al (1973). After alcohol precipitation, the DNA was dissolved in 0.1 M NaCl, 0.05 M Tris-HCl,1 10 mM Na2EDTA (pH 7.0) and incubated overnight at 37 °C in RNase (100 /ug/mL).…”

Section: Methodsmentioning

confidence: 99%

Procollagen complementary DNA, a probe for messenger RNA purification and the number of type I collagen genes

Frischauf¹,

Lehrach²,

Rosner³

et al. 1978

Biochemistry

View full text Add to dashboard Cite

Type I procollagen mRNAs were separated from contaminating low-abundance messenger and nuclear RNAs by chromatography over Sepharose 4B in 0.65 M NaCl at room temperature. All of 27S rRNA and four-fifths of procollagen mRNAs bind to Sepharose under these conditions, while 18S rRNA and about three-fourths of other poly(A)-containing RNAs do not bind. AMV reverse transcriptase was used to prepare complementary DNA to procollagen mRNA at each purification step. Hybridization studies, in RNA excess, were carried out to establish the enrichment at each step both with respect to total RNA and to poly(A)-containing RNA. While "purified" procollagen mRNA preparations still consist of about 50% 27S rRNA, over 80% of cDNA prepared from it back hybridizes to its template at a log of cr0t1/2 of -1.9. This type I procollagen cDNA hybridizes in DNA excess to DNA isolated from chicken erythrocytes and from embryonic chick calvaria at a log c0t1/2 of 3.1, demonstrating that procollagen cDNA is complementary to unique gene sequences in both tissues and that procollagen genes are not reiterated.

show abstract

Synthesis of a Deoxyribonucleic Acid Sequence Complementary to Ovalbumin Messenger Ribonucleic Acid and Quantification of Ovalbumin Genes

Cited by 146 publications

References 33 publications

Molecular cloning of ovomucoid gene sequences from partially purified ovomucoid messenger RNA

Molecular cloning of ovomucoid gene sequences from partially purified ovomucoid messenger RNA

The synthesis and properties of the complete complementary DNA transcript of ovalbumin mRNA

Procollagen complementary DNA, a probe for messenger RNA purification and the number of type I collagen genes

Contact Info

Product

Resources

About