Synthesis, DNA and BSA binding, in vitro anti-proliferative and in vivo anti-angiogenic properties of some cobalt(iii) Schiff base complexes

Abstract

In the recent times metal complexes with dual mechanisms of action, anti-cancer and anti-angiogenic, have gained substantial interest in the field of medicinal chemistry.

Water-Soluble Dioxidovanadium(V) Complexes of Aroylhydrazones: DNA/BSA Interactions, Hydrophobicity, and Cell-Selective Anticancer Potential

“…CD spectroscopy is an important technique for examining the change in the secondary structure of proteins after binding with the metal complexes . The CD spectra of free BSA and of BSA after binding with 1 – 5 are shown in Figure , where the two negative bands at 208 and 222 nm represent the characteristic α-helical structure of BSA .…”

“…CD spectroscopy is an important technique for examining the change in the secondary structure of proteins after binding with the metal complexes. 104 The CD spectra of free BSA and of BSA after binding with 1−5 are shown in Figure 8, where the two where n, l, and C p stand for the number of amino acid residues, the optical path length (in cm), and the analyte molar concentration, respectively. α-Helical contents of free BSA and complex-bound BSA were determined from mean MRE values at 208 and 222 nm, respectively, using eqs E and F. 106 Here, 4000 is the MRE value of the BSA at 208 nm, where the βform and random coil conformations cross, while 33000 is the MRE value of BSA at 208 nm where the conformation of the pure helix crosses.…”

Various coordination modes of new coumarin Schiff bases toward Cobalt (III) ion: Synthesis, spectral characterization, in vitro cytotoxic activity, and investigation of apoptosis

“…[ 33,38,50,51 ] The anticancer efficacy (IC 50 Value) of these Cobalt (III) complexes is significant than the cobalt (III) complexes containing tetradentate Schiffbases on A549 Cells (15.5 ± 1.3–27.4 ± 1.5 μM; Cisplatin 61.0 ± 1.5 μM). [ 20 ] Anticancer activity of the cobalt (III) complexes was found to be higher than the cobalt (II) complexes containing 2‐(5‐[trifluoromethyl]‐2‐methoxyphenylimino)methyl)‐4,6‐dichlorophenol or 2‐(5‐[trifluoromethyl]‐2 methoxyphenylimino)methyl)‐4‐bromo‐ 6‐methoxyphenol with A549 cells (21.80–23.99 μM; Cisplatin 6.95 μM). [ 52 ] Cobalt (II) benzhydrazone complexes showed significant activity (39.5 ± 1.0 μM to 58.9 ± 1.2 μM; Cisplatin 25.3 μM) on A549 Cells compared with the Co (III) complexes ( Co1‐ Co4 ).…”

“…[ 19 ] Co (III) bis (thiosemicarbazone) complexes (diacetyl bis (thiosemicarbazone), pyruvaldehyde bis (thiosemicarbazone), glyoxal bis (thiosemicarbazone)) were synthesized and evaluated for anticancer potential by King et al [ 8 ] In vitro antiproliferative and in vivo anti‐angiogenic properties of tetradentate Schiff base Co (III) complexes were studied against A549 and VERO cell lines. [ 20 ] In this connection, we have synthesized Co (III) complexes of 3‐acetyl‐6‐chloro‐chromene‐2‐one thiosemicarbazone/semicarbazone, 3‐acetyl‐7‐hydroxy‐chromene‐2‐one thiosemicarbazone/semicarbazone, characterized them spectroscopically and studied their anticancer activity against A549, MCF‐7, and HUVEC cells.…”

Computational study, synthesis and evaluation of active peptides derived from Parasporin-2 and spike protein from Alphacoronavirus against colorectal cancer cells

“…The assays for determining the localization of the peptides in the SW620 and SW480 cells were carried out as previously published [ 42 ]. In detail, the cells were seeded into chamber slides (2 × 10 5 cells/well) for 24 h, washed twice with PBS, and then incubated in the absence and presence of the most active rhodamine-labeled peptides for each of cell line, at a concentration twice the EC 50 for 1 h at 37°C in the dark.…”