Synthesis, Characterization of Dextran Hydrogels and Their in Vitro Release of Gentamycin Sulphate

Guo, Rui; Chen, Peizhe; Mo, Yunfei; Lan, Yong; Wang, Xue; Zhang, Yuanming

doi:10.5301/jabfm.5000233

Cited by 4 publications

(6 citation statements)

References 23 publications

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“…According to the FTIR of DEX-MA, the absorption bands at 1700 and 813 cm −1 ascribed to stretching vibrations of C=O and −C=CH 2 , respectively. In addition, the formation of DEX-MA was confirmed by 1 H-NMR results, 1 Figure 1D).…”

Section: Characterization Of Ccnc Dex-ma and Mpda-tpementioning

confidence: 54%

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Real-time and noninvasive tracking of injectable hydrogel degradation using functionalized AIE nanoparticles

et al. 2020

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Abstract Visually monitoring of the residual morphology and quantitatively determining the degradation degree of hydrogels applied in tissue repair therapy in a real-time and noninvasive manner were a crucial technological mean. Despite conventional organic fluorescent molecules commonly used as probe to capture the real-time clues of the labeled hydrogels, they still encounter obstacles, including intrinsic photobleaching, cytotoxicity, and unknown interference factor of degradation caused by the change from polymer structure of hydrogels, thus making it difficult to accurately obtain the information of the hydrogels in vivo. To address the hard nut, we designed the multifunctional hydrogel system with a real-time quantitative aggregation-induced emission fluorescent detection and photoacoustic imaging tracking based on tetraphenylethene (TPE) that possesses the trait of aggregation-induced emission and low photobleaching, bound on the surface of mesoporous dopamine microspheres (MPDAs), and subsequently loaded into the photo-crosslinked injectable hydrogels. In vitro results showed that MPDA-TPE had good compatibility, emitted strong fluorescence when embedded in hydrogels, and maintained stable fluorescence property unless the hydrogels were degraded. Meanwhile, a mathematical formula for the kinetic degradation of hydrogels was established between gravitational and visual degradation in vitro, which can be used to predict in vivo degradation. Furthermore, MPDA possessed the clear photoacoustic imaging effect to provide more accurate clues. The designed hydrogel system holds a potential role in prediction of the in vivo degradation of implanted materials in an accurate, convenient, and real-time noninvasive manner and is a meaningful treatment aid in tissue engineering.

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Section: Characterization Of Ccnc Dex-ma and Mpda-tpementioning

confidence: 54%

“…Biodegradable and biocompatible hydrogels are threedimensional molecule network with good swelling behavior in water [1][2][3]. Similar structure to the extracellular matrix environment received much attention for controlled drug carriers and tissue engineering applications [4].…”

Section: Introductionmentioning

confidence: 99%

Real-time and noninvasive tracking of injectable hydrogel degradation using functionalized AIE nanoparticles

et al. 2020

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“…Gnavi et al constructed a gelatin‐based hydrogel system loaded with vascular endothelial growth factor (VEGF) to accelerate nerve regeneration. Chang et al also found that genipin cross‐linked gelatin conduit could effectively promote peripheral nerve regeneration. Polyacrylamide (PAM) hydrogels are widely used in the field of peripheral nerve regeneration due to their good biocompatibility .…”

Section: Introductionmentioning

confidence: 99%

PAM/GO/gel/SA composite hydrogel conduit with bioactivity for repairing peripheral nerve injury

Chen

Zhao

Yan³

et al. 2019

J Biomedical Materials Res

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The effective repair and functional recovery after peripheral nerve injury is still a hot topic in clinical research. The aim of this study was to construct a bioactive composite hydrogel conduit as a three‐dimensional scaffold for the repair of rat sciatic nerve defects. The composite hydrogel conduit was fabricated by blending different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS) and injecting into a self‐made mold with an inner diameter of 2 mm and an outer diameter of 6 mm. Then the conduit was further modified with YIGSR peptide. The properties of the conduit were firstly characterized by morphology, swelling behaviors, tensile test and then the conduits were implanted into rat sciatic nerve gap to evaluate their effect on promoting nerve regeneration. The results of diameter analysis indicated that the wall of hydrogel conduit was uniform. The tensile test of the conduit showed that the hydrogel conduit had suitable elastic properties and good mechanical properties. Further on, hematoxylin–eosin staining, masson trichrome staining and immunohistochemistry staining showed that the conduit could effectively promote the regeneration of rat sciatic nerve. Thus, the study demonstrates that the as prepared hydrogel conduits here are beneficial for promoting nerve repair and may have potential application value in nerve regeneration. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1273–1283, 2019.

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“…Furthermore, the optimization of both density and 3D distribution of such molecules in the hydrogel structure needs to be tailored to the specific tissue to be regenerated. As an example, when delivered without stabilization, growth factors diffuse rapidly, undergo denaturation and consequently lose bioactivity under normal physiological conditions [30][31][32] . The suboptimal release of growth factors, at high concentration or over a prolonged time, may lead to serious side effects, including the growth of tumors 41,42 .…”

Section: Introductionmentioning

confidence: 99%

“…The issue of recreating the ECM’s complex chemical environment might possibly be addressed by the tunable and controlled release of encapsulated bioactive agents from hydrogel matrixes 6,9,14,19,31–40 , thus providing the necessary stimuli for cell migration, differentiation and production of neo-tissue growth or regeneration. The release of active molecules is limited by the difficulty of retaining their bioactivity and achieving in situ, optimal doses, gradients and timing.…”

Section: Introductionmentioning

confidence: 99%

Polysaccharide-based hydrogels with tunable composition as 3D cell culture systems

et al. 2018

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Background: To date, cell cultures have been created either on 2-dimensional (2D) polystyrene surfaces or in 3-dimensional (3D) systems, which do not offer a controlled chemical composition, and which lack the soft environment encountered in vivo and the chemical stimuli that promote cell proliferation and allow complex cellular behavior. In this study, pectin-based hydrogels were developed and are proposed as versatile cell culture systems. Methods: Pectin-based hydrogels were produced by internally crosslinking pectin with calcium carbonate at different initial pH, aiming to control crosslinking kinetics and degree. Additionally, glucose and glutamine were added as additives, and their effects on the viscoelastic properties of the hydrogels and on cell viability were investigated. Results: Pectin hydrogels showed in high cell viability and shear-thinning behavior. Independently of hydrogel composition, an initial swelling was observed, followed by a low percentage of weight variation and a steady-state stage. The addition of glucose and glutamine to pectin-based hydrogels rendered higher cell viability up to 90%-98% after 1 hour of incubation, and these hydrogels were maintained for up to 7 days of culture, yet no effect on viscoelastic properties was detected. Conclusions: Pectin-based hydrogels that offer tunable composition were developed successfully. They are envisioned as synthetic extracellular matrix (ECM) either to study complex cellular behaviors or to be applied as tissue engineering substitutes.

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Synthesis, Characterization of Dextran Hydrogels and Their in Vitro Release of Gentamycin Sulphate

Cited by 4 publications

References 23 publications

Real-time and noninvasive tracking of injectable hydrogel degradation using functionalized AIE nanoparticles

Real-time and noninvasive tracking of injectable hydrogel degradation using functionalized AIE nanoparticles

PAM/GO/gel/SA composite hydrogel conduit with bioactivity for repairing peripheral nerve injury

Polysaccharide-based hydrogels with tunable composition as 3D cell culture systems

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