PAM/GO/gel/SA composite hydrogel conduit with bioactivity for repairing peripheral nerve injury

Abstract

The effective repair and functional recovery after peripheral nerve injury is still a hot topic in clinical research. The aim of this study was to construct a bioactive composite hydrogel conduit as a three‐dimensional scaffold for the repair of rat sciatic nerve defects. The composite hydrogel conduit was fabricated by blending different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS) and injecting into a self‐made mold with an inner diameter of 2 mm and an outer diameter of 6 mm. Then the conduit was further modified with YIGSR peptide. The properties of the conduit were firstly characterized by morphology, swelling behaviors, tensile test and then the conduits were implanted into rat sciatic nerve gap to evaluate their effect on promoting nerve regeneration. The results of diameter analysis indicated that the wall of hydrogel conduit was uniform. The tensile test of the conduit showed that the hydrogel conduit had suitable elastic properties and good mechanical properties. Further on, hematoxylin–eosin staining, masson trichrome staining and immunohistochemistry staining showed that the conduit could effectively promote the regeneration of rat sciatic nerve. Thus, the study demonstrates that the as prepared hydrogel conduits here are beneficial for promoting nerve repair and may have potential application value in nerve regeneration. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1273–1283, 2019.

Conductive Hydroxyethyl Cellulose/Soy Protein Isolate/Polyaniline Conduits for Enhancing Peripheral Nerve Regeneration via Electrical Stimulation

“…In order to achieve the goal of ES for nerve repair, the corresponding conductive conduits should be developed and used (Nezakati et al, 2018;Chen et al, 2019). In this work, we constructed a new functional composite conduit using conductive material and natural polymer materials.…”

Brain Derived Neurotrophic Factor and Glial Cell Line-Derived Neurotrophic Factor-Transfected Bone Mesenchymal Stem Cells for the Repair of Periphery Nerve Injury

“…However, autograft requires sacrifice of a functional nerve, which may result in donor nerve sensory loss and neuropathic pain ( Guo et al, 2018 ; Wu et al, 2020 ). Hence, the use of nerve guide conduits could avoid these problems ( Chrzaszcz et al, 2018 ; Chen et al, 2019 ; Vijayavenkataraman et al, 2019 ). However, the reported nerve conduits could not fully meet the demands for quick and effective nerve repair ( Carvalho et al, 2019 ; Riccio et al, 2019 ).…”

Repair of Peripheral Nerve Injury Using Hydrogels Based on Self-Assembled Peptides

“…IKVAV can promote the adhesion, differentiation, and axon growth of neurons in the injured peripheral nerve area, inhibit the adhesion and differentiation of glial cells, and simulate the appropriate microenvironment needed for nerve cell growth during the process of peripheral nerve repair [23]. RGD and YIGSR can promote cell adhesion [24][25][26][27]. A self-assembled peptide was designed by Professor Zhai to better control the mechanical strength of the matrix and RGD concentrations, reaching conditions close to those in the extracellular matrix [28].…”