Conductive Hydroxyethyl Cellulose/Soy Protein Isolate/Polyaniline Conduits for Enhancing Peripheral Nerve Regeneration via Electrical Stimulation

Wu, Ping; Chen, Feixiang; Xiao, Ao; Du, Qiaoyue; Dong, Qi; Ke, Meifang; Liang, Xiao; Zhou, Qing; Chen, Yun

doi:10.3389/fbioe.2020.00709

Cited by 31 publications

(29 citation statements)

References 39 publications

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“…Due to differences in structure and function, damaged axons in the central nervous system (CNS) of adult mammals are usually unable to regenerate, while nerve recovery is generally very poor despite the ability of peripheral nerves to spontaneously regenerate after PNI ( Gu et al, 2014 ). Both electrical stimulation ( Balseanu et al, 2020 ; Wu et al, 2020 ) and acupuncture ( Wong et al, 2011 ; He et al, 2015 ) have beneficial effects on PNI ( He et al, 2015 ; Wu et al, 2020 ) or central nerve injury (CNI) ( Wong et al, 2011 ; Balseanu et al, 2020 ). EA, which is the combination of electrical stimulation and acupuncture, has therapeutic effects on both PNI ( Wu J. et al, 2018 ) and CNI ( Cai et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Electroacupuncture Promoted Nerve Repair After Peripheral Nerve Injury by Regulating miR-1b and Its Target Brain-Derived Neurotrophic Factor

et al. 2020

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Growing evidence indicates that electroacupuncture (EA) has a definite effect on the treatment of peripheral nerve injury (PNI), but its mechanism is not completely clear. MicroRNAs (miRNAs) are involved in the regulation of a variety of biological processes, and EA may enhance PNI repair by regulating miRNAs. In this study, the rat sciatic nerve injury model was treated with EA for 4 weeks. Acupoints Huantiao (GB30) and Zusanli (ST36) were stimulated by EA 20 min once a day, 6 days a week for 4 weeks. We found that EA treatment downregulated the expression of miR-1b in the local injured nerve. In vitro experiments showed that overexpression of miR-1b inhibited the expression of brain-derived neurotrophic factor (BDNF) in rat Schwann cell (SC) line, while BDNF knockdown inhibited the proliferation, migration, and promoted apoptosis of SCs. Subsequently, the rat model of sciatic nerve injury was treated by EA treatment and injection of agomir-1b or antagomir-1b. The nerve conduction velocity ratio (NCV), sciatic functional index (SFI), and S100 immunofluorescence staining were examined and showed that compared with the model group, NCV, SFI, proliferation of SC, and expression of BDNF in the injured nerves of rats treated with EA or EA + anti-miR-1b were elevated, while EA + miR-1b was reduced, indicating that EA promoted sciatic nerve function recovery and SC proliferation through downregulating miR-1b. To summarize, EA may promote the proliferation, migration of SC, and nerve repair after PNI by regulating miR-1b, which targets BDNF.

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Section: Discussionmentioning

confidence: 99%

Electroacupuncture Promoted Nerve Repair After Peripheral Nerve Injury by Regulating miR-1b and Its Target Brain-Derived Neurotrophic Factor

et al. 2020

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“…The gold standard of long nerve regeneration is autograft ( Yi et al, 2018 ; Vijayavenkataraman, 2020 ). However, autograft requires sacrifice of a functional nerve, which may result in donor nerve sensory loss and neuropathic pain ( Guo et al, 2018 ; Wu et al, 2020 ). Hence, the use of nerve guide conduits could avoid these problems ( Chrzaszcz et al, 2018 ; Chen et al, 2019 ; Vijayavenkataraman et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Brain Derived Neurotrophic Factor and Glial Cell Line-Derived Neurotrophic Factor-Transfected Bone Mesenchymal Stem Cells for the Repair of Periphery Nerve Injury

Zhang¹,

Wu²,

Chen³

et al. 2020

Front. Bioeng. Biotechnol.

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Peripheral nerve injury is a common clinical neurological disease. In our previous study, highly oriented poly ( L -lactic acid) (PLLA)/soy protein isolate (SPI) nanofiber nerve conduits were constructed and exhibited a certain repair capacity for peripheral nerve injury. In order to further improve their nerve repairing efficiency, the bone mesenchymal stem cells (BMSCs) overexpressing brain derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) were introduced into the conduits as seed cells and then were used to repair the 10-mm sciatic nerve defects in rats. The nerve repair efficiency of the functional nerve conduits was evaluated by gait experiment, electrophysiological test, and a series of assays such as hemotoxylin-eosin (HE) staining, immunofluorescence staining, toluidine blue (TB) staining, transmission electron microscopy (TEM) observation of regenerated nerve and Masson’s trichrome staining of gastrocnemius muscle. The results showed that the conduits containing BMSCs overexpressing BDNF and GDNF double-factors group had better nerve repairing efficiency than blank BMSCs and single BDNF or GDNF factor groups, and superior to autografts group in some aspects. These data demonstrated that BDNF and GDNF produced by BMSCs could synergistically promote peripheral nerve repair. This study shed a new light on the conduits and stem cells-based peripheral nerve repair.

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“…Since the conductive matrix is required for nerve impulse conduction, conductive HSPS conduits were fabricated by in-situ polymerization of aniline as our recent report [ 27 , 28 ]. As the addition of polyaniline, the biodegradable HEC/SPI conduit changed from primrose yellow to black ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…In our previous studies, hydroxyethyl cellulose (HEC)/soy protein isolate (SPI)/polyaniline (PANI) sponges (HSPS) showed good mechanical strength, biocompatibility and could be made into implantable and flexible biosensor [ 27 , 28 ]. This study is committed to assess the peripheral nerve repair capability of HSPS conduits combined with Schwann cells and VEGF-A.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive strategy of conduit guidance combined with VEGF producing Schwann cells accelerates peripheral nerve repair

Tong

Luo

et al. 2021

Bioactive Materials

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Peripheral nerve regeneration requires stepwise and well-organized establishment of microenvironment. Since local delivery of VEGF-A in peripheral nerve repair is expected to promote angiogenesis in the microenvironment and Schwann cells (SCs) play critical role in nerve repair, combination of VEGF and Schwann cells may lead to efficient peripheral nerve regeneration. VEGF-A overexpressing Schwann cells were established and loaded into the inner wall of hydroxyethyl cellulose/soy protein isolate/polyaniline sponge (HSPS) conduits. When HSPS is mechanically distorted, it still has high durability of strain strength, thus, can accommodate unexpected strain of nerve tissues in motion. A 10 mm nerve defect rat model was used to test the repair performance of the HSPS-SC (VEGF) conduits, meanwhile the HSPS, HSPS-SC, HSPS-VEGF conduits and autografts were worked as controls. The immunofluorescent co-staining of GFP/VEGF-A, Ki67 and MBP showed that the VEGF-A overexpressing Schwann cells could promote the proliferation, migration and differentiation of Schwann cells as the VEGF-A was secreted from the VEGF-A overexpressing Schwann cells. The nerve repair performance of the multifunctional and flexible conduits was examined though rat behavioristics, electrophysiology, nerve innervation to gastrocnemius muscle (GM), toluidine blue (TB) staining, transmission electron microscopy (TEM) and NF200/S100 double staining in the regenerated nerve. The results displayed that the effects on the repair of peripheral nerves in HSPS-SC (VEGF) group was the best among the conduits groups and closed to autografts. HSPS-SC (VEGF) group exhibited notably increased CD31 + endothelial cells and activation of VEGFR2/ERK signaling pathway in the regenerated nerve tissues, which probably contributed to the improved nerve regeneration. Altogether, the comprehensive strategy including VEGF overexpressing Schwann cells-mediated and HSPS conduit-guided peripheral nerve repair provides a new avenue for nerve tissue engineering.

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Conductive Hydroxyethyl Cellulose/Soy Protein Isolate/Polyaniline Conduits for Enhancing Peripheral Nerve Regeneration via Electrical Stimulation

Cited by 31 publications

References 39 publications

Electroacupuncture Promoted Nerve Repair After Peripheral Nerve Injury by Regulating miR-1b and Its Target Brain-Derived Neurotrophic Factor

Electroacupuncture Promoted Nerve Repair After Peripheral Nerve Injury by Regulating miR-1b and Its Target Brain-Derived Neurotrophic Factor

Brain Derived Neurotrophic Factor and Glial Cell Line-Derived Neurotrophic Factor-Transfected Bone Mesenchymal Stem Cells for the Repair of Periphery Nerve Injury

Comprehensive strategy of conduit guidance combined with VEGF producing Schwann cells accelerates peripheral nerve repair

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