Synthesis, Characterization, Antioxidant Activity, and Toxicity Properties of Tripolyphosphate Crosslinked Chitosan

Abstract

Chitosan is biopolymer obtained from chitin deacetylation reaction. Chitin is a constituent of shrimps’ outer shell. Chitosan modification using crosslinker agent could increase the active groups and stabilize the physical character. One of non-toxic crosslink agents was tripolyphosphate. This study aimed at synthesizing tripolyphosphate crosslinked chitosan (TPP-Cs) to test the antioxidant and toxicity. Synthesizing tripolyphosphate chitosan was conducted using ionic gelation method. The antioxidant activity test was studied from DPPH radicals scavenging and the ability to inhibit linoleic acid oxidation. The toxicity test was using BSLT (Brine Shrimp Lethality Test) method and Artemia salina leach nauplii. The study showed that TPP-Cs could be synthesized from pink shrimp and showed antioxidant activity. The activity was due to amino and hydroxyl groups. The BSLT method showed that TPP-Cs had the potential to be applied for antibacterial, antivirus, or anticancer products.

The nano mixture of ethanol extract ketumbar (Coriandrum sativum L.) seed and putri malu (Mimosa pudica L.) leaves potentially reduced blood glucose levels in hyperglycemia wistar rat

“…The flavonoid antioxidant activity is predominantly dictated by the structure of their molecules, particularly the composition of substituents present within their molecular framework [30]. The flavonoids entrapped within the carrier matrix exhibit inhibitory effects on degradation [31], and the liberation of flavonoids from nano-chitosan-tripolyphosphate-C. sativum seed and Mimosa pudica leaves extract ethanol, which is affixed to the surface/adsorbed, transpires via flavonoid decomposition or desorption facilitated by diffusion and erosion [19]. The immiscible polymer matrix hinders the dispersion of flavonoid molecules in an aqueous medium.…”

“…During the interim, the phenomenon of flavonoid liberation via attrition can be attributed to the gradual degradation of the protective coating layer. The nano chitosan-tripolyphosphate-C. sativum and Mimosa pudica L extract ethanol corroded as a result of a hydrolysis reaction that liberated flavonoids from the coating system [19]. Chitosan, a type of polysaccharide polymer, exhibits the ability to undergo expansion and gel formation upon exposure to acidic conditions [33].…”

“…Table 1 reveals that the zeta potential value of nano chitosan-tripolyphosphate-C. sativum and M. pudica extract ethanol is -1.62 mV, indicating that it has the capacity as an introduction to bioactive material [32]. Nano chitosan-tripolyphosphate has the smallest particle size, which is 119.3 nm, indicating that it has the most surface area of the three samples [19].…”

ANALYSIS OF THE ABILITY OF NANO ETHANOL EXTRACT OF CORIANDER (Coriandrum sativum L.) SEEDS TO REDUCE BLOOD GLUCOSE LEVELS IN HYPERGLYCEMIC WISTAR RATS

“…Hal ini dikarenakan nano ekstrak biji ketumbar mampu melindungi senyawa aktif dari biji ketumbar melalui proses enkapsulasi, sehingga mencegah terjadinya degradasi. Flavonoid yang terenkapsulasi dalam matriks pembawa dapat mencegah terjadinya degradasi (Batra et al, 2019) dan pelepasan flavonoid dari nano ekstrak biji ketumbar terikat pada permukaan/teradsorbsi yaitu terjadi melalui proses difusi (Setyaningtyas et al, 2021).…”