Synthesis, Characterization, Antibacterial, Antifungal, Antioxidant, and Anticancer Activities of Nickel-Doped Hydroxyapatite Nanoparticles

Abstract

The purpose of this research was to investigate the possible antibacterial, antifungal, antioxidant, and anticancer effects of nickel (Ni2+)-doped hydroxyapatite (HAp) nanoparticles (NPs) synthesized using the sol–gel approach. X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy (Raman), field-emission scanning electron microscopy (FESEM), and elemental analysis were used to characterize the Ni2+-doped HApNPs. X-ray diffraction investigation showed that the nanoscale structure of Ni2+-doped HApNPs was hexagonal, with an average crystallite size of 39.91 nm. Ni2+-doped HApNPs were found to be almost spherical in form and 40–50 nm in size, as determined by FESEM analysis. According to EDAX, the atomic percentages of Ca, O, P, and Ni were 20.93, 65.21, 13.32, and 0.55, respectively. Ni2+-doped HApNPs exhibited substantial antibacterial properties when tested in vitro against several pathogens, including Escherichia coli, Shigella flexneri, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus. Antibacterial activity, at 50 mg tested concentration, demonstrated superior effects on G-ve bacteria than G+ve pathogens. The antifungal activity of Oidium caricae, Aspergillus flavus, and A. niger revealed a zone of inhibition of 23, 11, and 5 mm, respectively. These actions rely on the organism’s cell wall structure, size, and shape. Incorporating Ni2+ into HApNPs allows them to function as powerful antioxidants. Ni2+-doped HApNPs had a good cytotoxic impact against the HeLa cell line, which improved with increasing concentration and was detected at a 68.81 µg/mL dosage. According to the findings of this study, the Ni2+-doped HApNPs are extremely promising biologically active candidates owing to their improved functional features.

Nutrient-Doped Hydroxyapatite: Structure, Synthesis and Properties

“…Thus, the importance of Ni dopants in HAP stricture is denoted by the applicability of these nanoparticles for medical applications as antibacterial agents. Moreover, these nanoparticles showed high levels of antifungal, antioxidant, and anticancer activities, making them highly promising for biomedical applications [97].…”

Nickel‐ion substituted hydroxyapatite matrices for metal‐affinity chromatographic purification of recombinant proteins

“…The replacement of C sites with various metal ions (e.g., Sr 2+ , Ag 2+ , Mg 2+ , Zn 2+ , Mn 2+ , Co 2+ , and Y 3+ ) has been reported for various applications, but the structural incorporation of Ni 2+ in HAp is very uncommon in existing literature [13]. Earlier reports on metal-substituted HAp materials have focused on biomaterials and water-treatment applications [14][15][16][17]. There are a few reports on batch adsorption studies with recombinant proteins [5,7,18].…”

Nanohydroxyapatite Loaded with 5-Fluorouracil and Calendula officinalis L. Plant Extract Rich in Myo-Inositols for Treatment of Ovarian Cancer Cells

“…Such compounds can be loaded into the nanocarriers for local therapy or diagnostics [16]. Besides many nanocarriers, one of the promising candidates is nanostructural hydroxyapatite (HAp), which is widely studied for several biological uses, including antibacterial, drug delivery, dental, and orthopedic applications [17]. Depending on its application, much attention has been paid to incorporating different metals into HAp to change its properties, e.g., HAp doped with Fe and Co displayed increased antibacterial activity against Shigella dysenteriae, while Mn doping improved activity against Staphylococcus aureus [18].…”