Synthesis, characterization and toxicological evaluation of Cr2O3 nanoparticles using Daphnia magna and Aliivibrio fischeri

Puerari, Rodrigo Costa; Costa, Cassiane da; Vicentini, Denice Schulz; Fuzinatto, Cristiane Funghetto; Melegari, Sílvia Pedroso; Schmidt, Éder C.; Bouzon, Zenilda L.; Matias, William Gerson

doi:10.1016/j.ecoenv.2016.02.011

Cited by 28 publications

(8 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Second, Cr contamination remains a serious environmental problem in several low-to middle-income countries whose economic activity is linked with the material demands of the current lifestyle in developed countries (Pure Earth and Green Cross 2016). Third, the safe-perspective use of Cr 2 O 3 NPs is linked to their intrinsic properties but also to the possible release of soluble Cr in either redox form (Horie et al 2013;da Costa et al 2016;Puerari et al 2016). In all cases, the appropriate knowledge of the actual ecotoxicity of Cr VI and Cr III necessary to assess the environmental risks of Cr appears incomplete.…”

Section: Implications For Cr Hazard and Risk Assessmentmentioning

confidence: 99%

Chromium hazard and risk assessment: New insights from a detailed speciation study in a standard test medium

Aharchaou

Cambier

et al. 2018

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Despite the consensus about the importance of chemical speciation in controlling the bioavailability and ecotoxicity of trace elements, detailed speciation studies during laboratory ecotoxicity testing remain scarce, contributing to uncertainty when extrapolating laboratory findings to real field situations in risk assessment. We characterized the speciation and ecotoxicological effects of chromium (Cr and Cr ) in the International Organization for Standardization (ISO) medium for algal ecotoxicity testing. Total and dissolved (< 0.22 μm) Cr concentrations showed little variability in media spiked with Cr , whereas dissolved Cr concentration decreased by as much as 80% over a 72-h time period in medium amended with Cr . Analyses by ion chromatography inductively coupled plasma mass spectrometry (IC-ICP-MS) highlighted the absence of redox interconversion between Cr or Cr both in the presence and absence of algal cells (Raphidocelis subcapitata). Furthermore, the concentration of ionic Cr dropped below detection limits in less than 2 h with the corresponding formation of carbonate complexes and Cr hydroxides. Precipitation of Cr in the form of colloidal particles of variable diameters was confirmed by nanoparticle (NP) tracking analysis, single particle ICP-MS, and single particle counting. In terms of time-weighted dissolved (< 0.22 μm) Cr concentration, Cr was 4 to 10 times more toxic than Cr . However, Cr ecotoxicity could arise from interactions between free ionic Cr and algae at the beginning of the test, from the presence of Cr-bearing NPs, or from a combination of the 2. Future ecotoxicological studies must pay more attention to Cr speciation to reliably compare the ecotoxicity of Cr and Cr . Environ Toxicol Chem 2018;37:983-992. © 2017 SETAC.

show abstract

Section: Implications For Cr Hazard and Risk Assessmentmentioning

confidence: 99%

Chromium hazard and risk assessment: New insights from a detailed speciation study in a standard test medium

Aharchaou

Cambier

et al. 2018

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

show abstract

“…reinhardtii , D. magna and A. fischeri (Puerari et al, 2016) and human cell cultures (Horie et al, 2013). Considering our results, studies on the Cr nanoparticles should systematically examine the bioavailability and toxicity of both forms of released Cr.…”

Section: Cr(iii) and Cr(vi) Accumulation By C Reinhardtiimentioning

confidence: 76%

Bioaccumulation and subcellular partitioning of Cr(III) and Cr(VI) in the freshwater green alga Chlamydomonas reinhardtii

et al. 2017

View full text Add to dashboard Cite

Chromium occurs in aquatic environments under two main redox forms, namely Cr(III) and Cr(VI), with different geochemical and biochemical properties. Cr(VI) readily crosses biological membranes of living organisms and once inside the cells it undergoes a rapid reduction to Cr(III). The route of entry for the latter form is, however, poorly known. Using the radioactive tracer Cr we compared the accumulation (absorption and adsorption) of the two Cr forms by the green unicellular alga Chlamydomonas reinhardii after 1h and 72h of exposure to 100nM of either Cr(III) or Cr(VI) at pH 7. Both Cr forms had similar accumulation, with a major part in the extracellular (adsorbed) fraction after 1h and a major part of total accumulated Cr in the intracellular (absorbed) fraction after 72h. We also investigated the intracellular partitioning of Cr using an operational fractionation scheme and found that both Cr forms had similar distributions among fractions: Cr was mostly associated with organelles (23±12% after 1h and 37±7% after 72h) and cytosolic heat-stable proteins and peptides (39±18% after 1h and 35±3% after 72h) fractions. Further investigations using a metallomic approach (SEC-ICP-MS) were performed with the heat-stable proteins and peptides fraction to compare the distribution of the two Cr forms among various biomolecules of this fraction. One Cr-binding biomolecule (∼28kDa) appeared after 1h of exposure for both Cr species. After 72h another biomolecule of lower molecular weight (∼0.7kDa) was involved in binding Cr and higher signal intensities were observed for Cr(VI) than for Cr(III). We show, for the first time, that both Cr(III) and Cr(VI) have similar fate within algal cells, supporting the tenet that a unique redox form occurs within cells.

show abstract

“…In the case of cobalt oxide NPs, various studies on zebrafish confirmed the non-lethal response of different types (Co 2 O 3 and Co 3 O 4 ) and shapes (spherical and block) of cobalt oxide NPs even at higher concentrations; 100 mg/L for 24 h, 100 mg/L for 96 h, 200 mg/L for 15 days [45,46,47]. In the case of acute toxicity of Cr 2 O 3 NPs to different aquatic organisms, Tavares et al [48] observed the mean half-maximal effective concentration (EC50-48h) of 6.74 mg/L for Daphnia similis while Puerari et al [49] obtained the EC 50-48 h value of 6.79 mg/L for Daphnia magna and EC 50-30 min value of 12.91 mg/L for Aliivibrio fischeri . Thus, the results of our and previous studies indicate that lethal concentrations and exposure period may differ for different species.…”

Section: Discussionmentioning

confidence: 99%

A Comparative Assessment of Nanotoxicity Induced by Metal (Silver, Nickel) and Metal Oxide (Cobalt, Chromium) Nanoparticles in Labeo rohita

et al. 2019

View full text Add to dashboard Cite

In the present in vivo study, we provide a comparison of toxicological consequences induced by four different types of spherical nanoparticles (NPs)—silver nanoparticles (AgNPs, 40 ± 6 nm), nickel (NiNPs, 43 ± 6 nm), cobalt oxide (Co3O4NPs, 60 ± 6 nm), and chromium oxide (Cr3O4NPs, 50 ± 5 nm)—on freshwater fish Labeo rohita. Fish were exposed to NPs (25 mg/L) for 21 days. We observed a NPs type-dependent toxicity in fish. An altered behavior showing signs of stress and a substantial reduction in total leukocyte count was noticed in all NP-treated groups. A low total erythrocyte count in all NP-treated fish except for Co3O4NPs was discerned while a low survival rate in the case of Cr3O4NP-treated fish was observed. A significant decrease in growth and hemoglobin were noticed in NiNP- and Cr3O4NP-treated fish. A considerable total protein elevation was detected in NiNP-, Co3O4NP-, and Cr3O4NP-treated groups. An upgrading in albumin level was witnessed in Co3O4NP- and Cr3O4NP-treated groups while a high level of globulin was noted in NiNP- and Co3O4NP-exposed groups. In all NP-treated groups, a depleted activity of antioxidative enzymes and pathological lesions in liver and kidney were noticed.

show abstract

Synthesis, characterization and toxicological evaluation of Cr2O3 nanoparticles using Daphnia magna and Aliivibrio fischeri

Cited by 28 publications

References 41 publications

Chromium hazard and risk assessment: New insights from a detailed speciation study in a standard test medium

Chromium hazard and risk assessment: New insights from a detailed speciation study in a standard test medium

Bioaccumulation and subcellular partitioning of Cr(III) and Cr(VI) in the freshwater green alga Chlamydomonas reinhardtii

A Comparative Assessment of Nanotoxicity Induced by Metal (Silver, Nickel) and Metal Oxide (Cobalt, Chromium) Nanoparticles in Labeo rohita

Contact Info

Product

Resources

About