Synthesis, characterization, and remodeling of weight-bearing allograft bone/polyurethane composites in the rabbit

Dumas, Jerald E.; Davis, Thomas E.; Holt, Ginger E.; Yoshii, Toshitaka; Perrien, Daniel S.; Nyman, Jeffry S.; Boyce, T. M.; Guelcher, Scott A.

doi:10.1016/j.actbio.2010.01.030

Cited by 39 publications

(53 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In general, their degradation rates decrease in the following order: PGA > PLA > PCL. Their blends have been shown to degrade faster than their pure counterparts (Dunn et al [2001]). Poly lactate-glycolic acid (PLGA) can completely degrade in several months in vivo , whereas poly-L-lactate (PLLA) and PCL take 3 to 5 years or more to completely degrade in vivo (Rich et al [2002]; Yang et al [2001]).…”

Section: Biodegradable and Surface Erodible Thermoplastic Polymersmentioning

confidence: 99%

Progress and challenges in biomaterials used for bone tissue engineering: bioactive glasses and elastomeric composites

Chen¹,

Zhu²,

Thouas³

2012

Prog Biomater

194

View full text Add to dashboard Cite

Driven by the increasing economic burden associated with bone injury and disease, biomaterial development for bone repair represents the most active research area in the field of tissue engineering. This article provides an update on recent advances in the development of bioactive biomaterials for bone regeneration. Special attention is paid to the recent developments of sintered Na-containing bioactive glasses, borate-based bioactive glasses, those doped with trace elements (such as Cu, Zn, and Sr), and novel elastomeric composites. Although bioactive glasses are not new to bone tissue engineering, their tunable mechanical properties, biodegradation rates, and ability to support bone and vascular tissue regeneration, as well as osteoblast differentiation from stem and progenitor cells, are superior to other bioceramics. Recent progresses on the development of borate bioactive glasses and trace element-doped bioactive glasses expand the repertoire of bioactive glasses. Although boride and other trace elements have beneficial effects on bone remodeling and/or associated angiogenesis, the risk of toxicity at high levels must be highly regarded in the design of new composition of bioactive biomaterials so that the release of these elements must be satisfactorily lower than their biologically safe levels. Elastomeric composites are superior to the more commonly used thermoplastic-matrix composites, owing to the well-defined elastic properties of elastomers which are ideal for the replacement of collagen, a key elastic protein within the bone tissue. Artificial bone matrix made from elastomeric composites can, therefore, offer both sound mechanical integrity and flexibility in the dynamic environment of injured bone.Electronic supplementary materialThe online version of this article (doi:10.1186/2194-0517-1-2) contains supplementary material, which is available to authorized users.

show abstract

Section: Biodegradable and Surface Erodible Thermoplastic Polymersmentioning

confidence: 99%

Progress and challenges in biomaterials used for bone tissue engineering: bioactive glasses and elastomeric composites

Chen¹,

Zhu²,

Thouas³

2012

Prog Biomater

194

View full text Add to dashboard Cite

show abstract

“…34 After curing, the complete disappearance of isocyanate groups and the formation of urethane groups were observed by infrared spectroscopy, while the peaks at 1536 and 1703 cm 21 indicated the presence of hydrogen-bonded urethane carbonyl groups which confirm the conversion toward polyurethane. 38,39 The observed pore sizes ranged between 100 and 600 mm which corresponds to the range of pore sizes considered suitable for bone regeneration, that is, between 100 and 700 mm. 21,[39][40][41][42] The incorporation of SrHA or HA nanoparticles in PU did not alter the pore size, but the mechanical properties decreased with increasing SrHA or HA content in PU due to the increase in porosity.…”

Section: Discussionmentioning

confidence: 98%

“…38,39 The observed pore sizes ranged between 100 and 600 mm which corresponds to the range of pore sizes considered suitable for bone regeneration, that is, between 100 and 700 mm. 21,[39][40][41][42] The incorporation of SrHA or HA nanoparticles in PU did not alter the pore size, but the mechanical properties decreased with increasing SrHA or HA content in PU due to the increase in porosity. Yoshii et al 43 showed that the incorporation of tricalcium phosphate microparticles (100-300 mm) into PU scaffolds did not significantly change the elastic modulus and compressive strength of polymer composites with increasing filler content.…”

Section: Discussionmentioning

confidence: 98%

Development of porous polyurethane/strontium‐substituted hydroxyapatite composites for bone regeneration

Sariibrahimoğlu

Yang

Leeuwenburgh

et al. 2014

J Biomedical Materials Res

View full text Add to dashboard Cite

Polyurethane (PU) has been widely used for the biomedical applications but its potential for bone regeneration is limited due to its lack of osteoconductive properties. Strontium substituted hydroxyapatite (SrHA) particles, on the other hand, are known to exhibit a positive effect on bone formation. Therefore, the aim of this study was to (i) develop porous polyurethane scaffolds containing strontium SrHA nanoparticles (PU/SrHA) and (ii) compare their in vitro biological performance for applications in bone regeneration to PU scaffolds. SrHA and HA was synthesized using a conventional wet-chemical neutralization reaction at temperatures of 25, 50, and 80°C. Chemical analysis was performed by inductively coupled plasma-optical emission spectrometry. Synthesizing temperatures at 25 and at 50°C were selected for the composite preparation (abbreviated as HA-25, SrHA-25, HA-50, and SrHA-50, respectively). PU was synthesized from isophorone diisocyanate, polytetramethylene ether glycol, and 1,4-butanediol. Composite scaffolds were prepared by addition of HA or SrHA nanoparticles into PU scaffolds during polymer preparation. The results showed that the Sr content in HA nanoparticles increased with increasing synthesis temperature. The addition of nanoparticles decreased the elongation-at-break and tensile strength, but significantly increased the surface wettability of the PU scaffolds. In vitro degradation tests demonstrated that release of cations was significantly higher from PU/SrHA-50 composite scaffolds. Cell culture tests indicated that PU composites containing either HA or SrHA nanoparticles increased proliferation of bone marrow stem cells as compared to plain PU scaffolds, whereas osteogenic differentiation was not affected by the incorporation of HA nanoparticles irrespective of the incorporation of Sr.

show abstract

“…Nevertheless, residual solvent may have been present. With regard to gamma irradiation, we have previously shown that irradiation of the either the reactive components of the PUR [23] or the cured PUR [24] at doses as high as 25 kGy does not affect their physical, mechanical, or biological properties. However, those studies involved different isocyanates and chain extenders than those used in this study.…”

Section: Discussionmentioning

confidence: 99%

“…(Previous studies have shown that γ-irradiation of the PUR or the components used to synthesize the PUR at doses as high as 25kGy does not alter the mechanical, physical, or biological properties of the material [23, 24]. )…”

Section: Methodsmentioning

confidence: 99%

Degradable Segmented Polyurethane Elastomers for Bone Tissue Engineering: Effect of Polycaprolactone Content

Kavlock

Whang

Guelcher

et al. 2012

Journal of Biomaterials Science, Polymer Edition

Self Cite

View full text Add to dashboard Cite

Segmented polyurethanes (PURs) – consisting of degradable poly(α-hydroxy ester) soft segments and amino acid-derived chain extenders – are biocompatible elastomers with tunable mechanical and degradative properties suitable for a variety of tissue engineering applications. In this study, a family of linear PURs synthesized from poly(ε-caprolactone) (PCL) diol, 1,4-diisocyanobutane and tyramine with theoretical PCL contents of 65 to 80 wt% were processed into porous foam scaffolds and evaluated for their ability to support osteoblastic differentiation in vitro. Differential scanning calorimetry and mechanical testing of the foams indicated increasing polymer crystallinity and compressive modulus with increasing PCL content. Next, bone marrow stromal cells (BMSCs) were seeded into PUR scaffolds – as well as poly(lactic-co-glycolic acid) (PLGA) scaffolds – and maintained under osteogenic conditions for 14 and 21 days. Analysis of cell number indicated a systematic decrease in cell density with increasing PUR stiffness at both 14 and 21 days in culture. However, at these same time points the relative mRNA expression for the bone-specific proteins osteocalcin and the growth factors bone morphogenetic protein-2 and vascular endothelial growth factor gene expression were similar among the PURs. Finally, prostaglandin E2 production, alkaline phosphatase activity, and osteopontin mRNA expression were highly elevated on the most-crystalline PUR scaffold as compared to the PLGA and PUR scaffolds. These results suggest that both the modulus and crystallinity of the PUR scaffolds influence cell proliferation and the expression of osteoblastic proteins.

show abstract

Synthesis, characterization, and remodeling of weight-bearing allograft bone/polyurethane composites in the rabbit

Cited by 39 publications

References 56 publications

Progress and challenges in biomaterials used for bone tissue engineering: bioactive glasses and elastomeric composites

Progress and challenges in biomaterials used for bone tissue engineering: bioactive glasses and elastomeric composites

Development of porous polyurethane/strontium‐substituted hydroxyapatite composites for bone regeneration

Degradable Segmented Polyurethane Elastomers for Bone Tissue Engineering: Effect of Polycaprolactone Content

Contact Info

Product

Resources

About