Cotton fabrics with antimicrobial properties were prepared by treatment with aqueous solutions of poly vinyl alcohol/zinc oxide (PVA/ZnO) nanocomposites and subsequently exposed to UV radiation. The ZnO nanoparticles, PVA/ZnO nanocomposite films, and treated cotton fabrics were characterized by transmission electron microscopy, Fourier transform infrared, X-ray diffraction, scanning electron microscopy, and thermogravimetric analysis. The antibacterial finishing of the treated cotton fabrics was evaluated toward gram-positive and gram-negative bacteria. J. VINYL ADDIT. FIG. 3. The X-ray diffraction patterns of pure poly vinyl alcohol and poly vinyl alcohol/zinc oxide nanocomposite films. FIG. 4. Scanning electron microscopy of (a) untreated cotton fabrics, (b) poly vinyl alcohol/zinc oxide nanocomposite treated samples.