Synthesis, Characterization, and In Situ Antifungal and Cytotoxicity Evaluation of Ascorbic Acid-Capped Copper Nanoparticles

Beltrán-Partida, Ernesto; Valdez‐Salas, Benjamín; Valdez-Salas, Ernesto; Pérez-Cortéz, Guillermo; Nedev, N.

doi:10.1155/2019/5287632

Cited by 20 publications

(10 citation statements)

References 40 publications

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“…The significance of differences was tested using one-way analysis of variance (ANOVA) followed by Tukey´s multiple comparison tests, and Student t -Test when appropriate. 29 A P < 0.05 was considered statistically significant.…”

Section: Methodsmentioning

confidence: 99%

Promotion of Surgical Masks Antimicrobial Activity by Disinfection and Impregnation with Disinfectant Silver Nanoparticles

Valdez‐Salas

Beltrán-Partida

Cheng³

et al. 2021

IJN

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Background The COVID-19 pandemic is requesting highly effective protective personnel equipment, mainly for healthcare professionals. However, the current demand has exceeded the supply chain and, consequently, shortage of essential medical materials, such as surgical masks. Due to these alarming limitations, it is crucial to develop effective means of disinfection, reusing, and thereby applying antimicrobial shielding protection to the clinical supplies. Purpose Therefore, in this work, we developed a novel, economical, and straightforward approach to promote antimicrobial activity to surgical masks by impregnating silver nanoparticles (AgNPs). Methods Our strategy consisted of fabricating a new alcohol disinfectant formulation combining special surfactants and AgNPs, which is demonstrated to be extensively effective against a broad number of microbial surrogates of SARS-CoV-2. Results The present nano-formula reported a superior microbial reduction of 99.999% against a wide number of microorganisms. Furthermore, the enveloped H5N1 virus was wholly inactivated after 15 min of disinfection. Far more attractive, the current method for reusing surgical masks did not show outcomes of detrimental amendments, suggesting that the protocol does not alter the filtration effectiveness. Conclusion The nano-disinfectant provides a valuable strategy for effective decontamination, reuse, and even antimicrobial promotion to surgical masks for frontline clinical personnel.

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Section: Methodsmentioning

confidence: 99%

Promotion of Surgical Masks Antimicrobial Activity by Disinfection and Impregnation with Disinfectant Silver Nanoparticles

Valdez‐Salas

Beltrán-Partida

Cheng³

et al. 2021

IJN

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“…As a result, the reaction driving force is modest and Cu nanoparticle aggregation is di cult. The chemical reduction method for synthesis of copper nanoparticle was also supported by earlier study (Aguilar et al 2019;Beltrán-Partida et al 2019).…”

Section: Discussionmentioning

confidence: 74%

Deciphering the molecular insight behind the inhibitory role of copper and silver nanocomposite on important bacterial and fungal pathogens in rice (oryza sativa)

Chowdhury

Kumar

Mahanty

et al. 2022

Preprint

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Rice (Oryza sativa) being among the most important food crops in the world is also susceptible to various bacterial and fungal diseases that are the major stumbling blocks in the way of increased production and productivity. The bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae and the sheath blight disease caused by Rhizoctonia solani are among the most devastating diseases of the rice crop. In spite of the availability of array of chemical control, there are chances of development of resistance. Thus, there is a need for the nanotechnological intervention for management of disease in the form of copper and silver nano-composites. The copper and silver nanoparticles were synthesized and characterized using different high throughput techniques, i.e., UV-Vis, FT-IR, DLS, XRD, FE-SEM, TEM. The characterization results confirmed the formation of copper and silver nanoparticles. The copper and silver nanoparticle composites evaluated against Xanthomonas oryzae pv. oryzae and Rhizoctonia solani were found to have higher antibacterial and antifungal activities compared to only the copper nanoparticle. Net house trials of nano-composite formulations against the bacterial blight of rice also corroborated the potential of nanocomposite formulation. In silico studies were carried out selecting two disease-causing proteins, peptide deformylase (Xanthomonas oryzae) and pectate lyase (Rhizoctonia solani) to perform the molecular docking and interaction studies with silver and copper nanoparticles. These proteins could be the potential targets of the nanoparticles. The study suggested that the copper and silver nano-composites could be used for developing formulations to control these devastating rice diseases.

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“…Figure 9 depicts the antifungal mechanism of CuNPs against Corynespora cassiicola and Neoscytalidium dimidiatum , including direct adherence of CuNPs to the fungal surface and the influence on membrane structural integrity. 79 CuNPs interact with the fungal cell wall due to their affinity for the carboxyl group found on the fungal surface. 80 The antifungal action of nanoparticles is attributed to the generation of reactive oxygen species (ROS), membrane damage, loss of enzyme activity, protein malfunction, and other factors.…”

Section: Resultsmentioning

confidence: 99%

Durian Shell-Mediated Simple Green Synthesis of Nanocopper against Plant Pathogenic Fungi

Duong

Nguyen

Tran³

et al. 2023

ACS Omega

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The synthesis of fungicides in eco-friendly and costeffective ways is significantly essential for agriculture. Plant pathogenic fungi cause many ecological and economic issues worldwide, which must be treated with effective fungicides. Here, this study proposes the biosynthesis of fungicides, which combines copper and Cu 2 O nanoparticles (Cu/Cu 2 O) synthesized using durian shell (DS) extract as a reducing agent in aqueous media. Sugar and polyphenol compounds contained in DS, as the main phytochemicals acting in the reduction procedure, were extracted under different temperatures and duration conditions to obtain the highest yields. We confirmed the extraction process performed at 70 °C for 60 min to be the most effective in extracting sugar (6.1 g/L) and polyphenols (22.7 mg/L). We determined the suitable conditions for Cu/Cu 2 O synthesis using a DS extract as a reducing agent for a synthesis time of 90 min, a volume ratio of DR extract/Cu 2+ of 15:35, an initial pH solution of 10, a synthesis temperature of 70 °C, and a CuSO 4 concentration of 10 mM. The characterization results of as-prepared Cu/Cu 2 O NP showed a highly crystalline structure of Cu 2 O and Cu with sizes estimated in the range of 40−25 nm and 25−30 nm, respectively. Through in vitro experiments, the antifungal efficacy of Cu/Cu 2 O against Corynespora cassiicola and Neoscytalidium dimidiatum was investigated by the inhibition zone. The green-synthesized Cu/Cu 2 O nanocomposites, which are potential antifungals against plant pathogens, exhibited excellent antifungal efficacy against both Corynespora cassiicola (MIC = 0.25 g/L, the diameter of the inhibition zone was 22.00 ± 0.52 mm) and Neoscytalidium dimidiatum (MIC = 0.0625 g/L, the diameter of the inhibition zone was 18.00 ± 0.58 mm). Cu/Cu 2 O nanocomosites prepared in this study could be a valuable suggestion for the control of plant pathogenic fungi affecting crop species globally.

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Synthesis, Characterization, and In Situ Antifungal and Cytotoxicity Evaluation of Ascorbic Acid-Capped Copper Nanoparticles

Cited by 20 publications

References 40 publications

Promotion of Surgical Masks Antimicrobial Activity by Disinfection and Impregnation with Disinfectant Silver Nanoparticles

Promotion of Surgical Masks Antimicrobial Activity by Disinfection and Impregnation with Disinfectant Silver Nanoparticles

Deciphering the molecular insight behind the inhibitory role of copper and silver nanocomposite on important bacterial and fungal pathogens in rice (oryza sativa)

Durian Shell-Mediated Simple Green Synthesis of Nanocopper against Plant Pathogenic Fungi

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