Synthesis, Characterization, and In Vitro and In Vivo Evaluations of Cellulose Hydrogels Enriched with Larrea tridentata for Regenerative Applications

Abstract

Introduction. Tissue engineering is an elementary necessity for several applications in the biomedical field through the use of several biopolymers derived from plants. Larrea tridentata (LT) is a very used plant for various medicinal applications with interesting properties; however, its use into cellulose hydrogels for possible regenerative therapeutics is still limited. Cellulose films could be applied in medical field as wound healing, scaffold for connective tissue for periodontal applications, and so on. The aim of this study was to evaluate the mechanical properties and in vivo and in vitro biocompatibility of cellulose hydrogels that have been enriched with LT in a rat model. Methods. By in vivo and in vitro assays, the concentration of LT was varied from 1 to 5 wt%, respectively. Hydrogel films were implanted intramuscularly into female Wistar rats, 250 g in weight and aged 2 months, to analyze their cytocompatibility and biocompatibility. Results. No case showed any evidence of inflammation or toxicity. Regarding cell morphology and adhesion, the prepared LT cellulose films had better cytocompatibility values than when polystyrene (PS) dishes were used as the control. In all cases, the results suggest that the addition of LT to the hydrogel films did not affect their cytocompatibility or biocompatibility properties and increases their clinical application due to the reported uses of LT. Conclusions. Cellulose hydrogel films enriched with LT have the potential to be used in the biomedical field acting as regenerative scaffolds.

Bactericidal Activity of Larrea tridentata Hydroalcoholic Extract against Phytopathogenic Bacteria

“…Several studies have reported the pharmacological properties of Larrea tridentata, such as antioxidant [19][20][21][22][23][24][25], antitumoral [23], neuroprotective [26,27], regenerative [28], hepatoprotective [29][30][31], nephroprotective [32,33], antiviral [34], antifungal (mainly against fungi associated with agricultural crops, including tomatoes) [22,[35][36][37][38], antiparasitic [39], and antibacterial (against Gram-positive and Gram-negative bacteria and Mycobacteria) effects [40][41][42][43][44][45][46][47][48][49]. However, there are no reports on the antibacterial activity of this plant against pathogens that affect agricultural production, so the aim of the present study was to evaluate the antibacterial activity of hydroalcoholic extract and fractions of Larrea tridentata against phytopathogenic bacteria.…”

Phytochemical Compounds and Pharmacological Properties of Larrea tridentata

“…Tovar-Carrillo et al (2020) analyzed the compatibility of the in vitro and in vivo properties of cellulose hydrogels enriched with L. tridentata that had been implanted intramuscularly in female rats. At the end of the in vivo assay (90 days), no evidence of inflammation, toxicity or death was observed; furthermore, it was observed that the addition of L. tridentata improved cytocompatibility, demonstrating that enriched hydrogels can be used as regenerative scaffolds [ 40 ].…”