Synthesis, characterization and evaluation of cell culture study of mercury complex derived from benzene-1,3,5-tricarboxylic acid

Abstract

Mercury complexes have diverse effects on the human body and cells that depend upon the biochemical form of mercury-complexes and the nature of exposure. In the present work, we have investigated the impacts of mercury-complex derived from benzene-1,3,5-tricarboxylic acid on cell culture and DNA damage. This is novel mercury-complex having cell culture study. The mercury complex has been synthesized and characterized by CHNS analyzer, FTIR, X-Ray Diffraction (XRD) and DNA damage. Surface morphology of prepared mercury-complex was studied by microscopy imaging/Atomic Force Microscopy (AFM). The main goal of this contribution is to address the damaging effects of mercury-complex in cell cultures through fluorescence imaging and identifying cell Live/Death quantitative results. These live/death color intensities are red or green in presence to the mercury-complex. For this purpose, we measured the concentration dependence of mercury-complex on the rate of death in cells which may be useful for the cell culture and DNA study. The mercury-complex derived from benzene-1,3,5-tricarboxylic acid has the ability to break the polynucleotide structure of DNA to mono nucleotides resulting irreparable DNA damage. The experimental results of fluorescence microscopy and live/dead cell assay on cell viability reflected the potent cellular toxicity of mercury-complex causes cell culture study. Generally, the damaging effects of mercury-complex may be used for medical treatment of different diseases specially cancer.