Synthesis, Characterization, and Evaluation as Transfection Reagents of Double-Hydrophilic Star Copolymers:  Effect of Star Architecture

Georgiou, Theoni K.; Vamvakaki, Maria; Phylactou, Leonidas A.; Patrickios, Costas S.

doi:10.1021/bm050307w

Cited by 97 publications

(131 citation statements)

References 38 publications

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“…The conformational change of the DMAEMA star size is connected with the change in the degree of ionization of arms. The effective pK values of the ionizable amine groups in DMAEMA star polymers, may be found in the literature, to be around 7 [7,8]. No abrupt changes of star sizes is seen in this region of pH.…”

Section: Ph Sensitivity Of Stars In Watermentioning

confidence: 72%

“…Polycationic star polymers used for the formation of polyplexes are mainly made of polyethyleneimine [6], poly(N,N-dimethylaminoethyl methacrylate) (PDMAEMA) [7][8][9] and poly(N,N-dimethylaminopropylacrylamide) [10]. The most popular are PDMAEMA stars.…”

Section: Introductionmentioning

confidence: 99%

“…PDMAEMA stars have been obtained via an "arm-first" method by group transfer polymerization, in which ethylene glycol dimethacrylate or bis(methacryloyloxyethyl) methylamine was used as the crosslinking agent to interconnect individual PDMAEMA chains at one of their ends [7,8]. In "the core first" approach, multifunctional cores have been used as macroinitiators for the polymerization of the arms.…”

Section: Introductionmentioning

confidence: 99%

“…The condensation of DNA is more efficient for PDMAEMA star polymers in comparison to linear analogues [9,16]. The dense molecular star structure and the higher number of functional groups for a given degree of polymerization compared to linear polymers used in polyplexes have improved the effectiveness of stars for DNA complexation [7,8]. Because of their small sizes, stars can form smaller polyplexes, which are believed to be more desirable for achieving high transfection efficiency [9,32].…”

Section: Introductionmentioning

confidence: 99%

“…It is known that transfection efficiency increases with the polymer concentration, but this increase is accompanied by a decrease in cell viability [7,8,16]. In general, cytotoxicity increases with the increasing molar mass of the polycationic polymer.…”

Section: Introductionmentioning

confidence: 99%

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Polycationic star polymers with hyperbranched cores for gene delivery

Mendrek

Sieroń

Libera

et al. 2014

Polymer

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Core-shell type stars synthesized via atom transfer radical polymerization were used for the delivery of nucleic acids. The interior of the stars consisted of hyperbranched poly(arylene oxindole), while the arms were composed of poly(N,N-dimethylaminoethyl methacrylate).The length of the star arms varied in degree of polymerization (DP) from 14 to 98. The hydrodynamic radius of the structures measured in water indicated the presence of small aggregates, while isolated stars ranging in size from 14 to 29 nm were seen in organic solvent.The phase transition temperatures of the stars in water, measured in basic conditions, were shifted to lower values with increasing DP of the arms. Stable polyplexes of stars with plasmid DNA were formed. Their size varied from 300 nm to 400 nm, depending upon the DP of arms. The zeta potential of the polyplexes was positive, which facilitated their cellular uptake. The DP of the arms influenced the transfection efficiency of HT-1080 cells, demonstrating that stars are promising candidates for synthetic gene vectors.

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Section: Ph Sensitivity Of Stars In Watermentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Polycationic star polymers with hyperbranched cores for gene delivery

Mendrek

Sieroń

Libera

et al. 2014

Polymer

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Group Transfer Polymerization

Rikkou-Kalourkoti¹,

Webster²,

Patrickios³

2013

Encyclopedia of Polymer Science and Technology

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GROUP TRANSFER POLYMERIZATIONVol. 99 simplicity and robustness of controlled radical polymerizations in combination with the sensitivity of GTP to moisture and labile protons have restricted the employment of GTP for macromolecular engineering during the past 15 years. However, GTP is still attractive to several polymer synthesis research teams as it rapidly provides polymer products more homogeneous (Ð ∼1.2-1.3) than those of controlled radical polymerizations (Ð ∼1.2-1.6).The recent development of new superior catalysts for GTP provided new impetus for this polymerization method. The most noteworthy advancement was the demonstration by the groups of D. Taton (University of Bordeaux, France) and R. M. Waymouth (Stanford University, California) that NHCs equally and efficiently catalyze the GTP of acrylates, methacrylates, and acrylamides, and enable the synthesis of their block copolymers at any block sequence. Other important contributions have also been recently reported by the Groups of E. Y.-X. Chen (Colorado State University at Fort Collins, Colorado) and T. Kakuchi (Hokkaido University, Japan). Note, however, that GTP described in this article is not related to the rare earth metal-mediated group transfer polymerization developed by Yasuda in 1992 (23).This article is not intended to be a comprehensive review of GTP, but rather a brief update of an early review (24). For a complete account, the interested reader is referred to the various reviews published on the topic (25-27), and, in particular, the very recent one by Kakuchi and co-workers (28).

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From Biomineralization Polymers to Double Hydrophilic Block and Graft Copolymers

Cölfen

2007

Macromolecular Engineering

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Synthesis, Characterization, and Evaluation as Transfection Reagents of Double-Hydrophilic Star Copolymers: Effect of Star Architecture

Cited by 97 publications

References 38 publications

Polycationic star polymers with hyperbranched cores for gene delivery

Polycationic star polymers with hyperbranched cores for gene delivery

Group Transfer Polymerization

From Biomineralization Polymers to Double Hydrophilic Block and Graft Copolymers

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