Synthesis, characterization, and cytotoxicity of glutathione-PEG-iron oxide magnetic nanoparticles

Haddad, Paula S.; Santos, Marconi C.; Cassago, Carolina Aparecida de Guzzi; Bernardes, Juliana S.; Jesus, Marcelo Bispo de; Seabra, Amedea B.

doi:10.1007/s11051-016-3680-y

Cited by 19 publications

(9 citation statements)

References 70 publications

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“…Higher hydrodynamic sizes of NPs measured by DLS, in comparison with the sizes obtained by AFM or XRD, are attributed to the presence of extra hydrate layers in aqueous environments. 10,18,19 The zeta potential was -22.10 AE 0.55 mV. These results indicate that thiolated nanoparticles have a hydrodynamic diameter in the nanoscale and a moderate polydispersity index, indicating a good dispersion in water, which makes these NPs attractive vehicles for drug delivery in biomedical applications.…”

Section: Synthesis and Characterization Of Fe 3 O 4 -Msa Npsmentioning

confidence: 76%

“…Our group has been synthesizing Fe 3 O 4 NPs by coprecipitation and thermal decomposition methods, followed by NP surface functionalization with different coatings that can act as carriers of nitric oxide (NO), including thiol molecules such as mercaptosuccinic acid (MSA), dimercaptosuccinic acid (DMSA), [11][12][13][14] glutathione (GSH), cysteine (Cys), 8,9,15 silica, 16,17 and polyethylene glycol (PEG). 18,19 Nitric oxide (NO) is an important biological molecule involved in several physiological processes such as the promotion of wound healing, the control of blood ow, and antimicrobial and anticancer activities. [20][21][22][23][24] However, as a free radical, the potential therapeutic applications of NO have been limited by its instability and short half-life.…”

Section: Introductionmentioning

confidence: 99%

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Superparamagnetic iron oxide nanoparticles dispersed in Pluronic F127 hydrogel: potential uses in topical applications

et al. 2017

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Section: Synthesis and Characterization Of Fe 3 O 4 -Msa Npsmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Superparamagnetic iron oxide nanoparticles dispersed in Pluronic F127 hydrogel: potential uses in topical applications

et al. 2017

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“…Total levels of NO were quantified by measuring nitrite (NO 2 − ), nitrate (NO 3 − ), and S-nitrosothiol contents by using the WPI TBR4100/1025 amperometric NO sensor (World Precision Instruments Inc., Sarasota, FL, USA) with the nitric oxide specific ISO-NOP sensor (2 mm) [ 30 , 31 ]. For quantification of S-nitrosothiols, a solution of CuCl 2 (100 mmol L −1 ) was used, and for NO 2 − , a solution of potassium iodide (KI, 100 mmol·L −1 ) in sulfuric acid (H2SO 4 , (100 mmol·L −1 ) was used.…”

Section: Methodsmentioning

confidence: 99%

Mitochondrial Dysfunction in Cardiorenal Syndrome 3: Renocardiac Effect of Vitamin C

Neres-Santos

Junho

Panico

et al. 2021

Cells

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Cardiorenal syndrome (CRS) is a pathological link between the kidneys and heart, in which an insult in a kidney or heart leads the other organ to incur damage. CRS is classified into five subtypes, and type 3 (CRS3) is characterized by acute kidney injury as a precursor to subsequent cardiovascular changes. Mitochondrial dysfunction and oxidative and nitrosative stress have been reported in the pathophysiology of CRS3. It is known that vitamin C, an antioxidant, has proven protective capacity for cardiac, renal, and vascular endothelial tissues. Therefore, the present study aimed to assess whether vitamin C provides protection to heart and the kidneys in an in vivo CRS3 model. The unilateral renal ischemia and reperfusion (IR) protocol was performed for 60 min in the left kidney of adult mice, with and without vitamin C treatment, immediately after IR or 15 days after IR. Kidneys and hearts were subsequently collected, and the following analyses were conducted: renal morphometric evaluation, serum urea and creatinine levels, high-resolution respirometry, amperometry technique for NO measurement, gene expression of mitochondrial dynamic markers, and NOS. The analyses showed that the left kidney weight was reduced, urea and creatinine levels were increased, mitochondrial oxygen consumption was reduced, NO levels were elevated, and Mfn2 expression was reduced after 15 days of IR compared to the sham group. Oxygen consumption and NO levels in the heart were also reduced. The treatment with vitamin C preserved the left kidney weight, restored renal function, reduced NO levels, decreased iNOS expression, elevated constitutive NOS isoforms, and improved oxygen consumption. In the heart, oxygen consumption and NO levels were improved after vitamin C treatment, whereas the three NOS isoforms were overexpressed. These data indicate that vitamin C provides protection to the kidneys and some beneficial effects to the heart after IR, indicating it may be a preventive approach against cardiorenal insults.

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“…Vinluan et al [22] has shown that the surface modification with Glutathione (GT), an endogenous antioxidant composed of glutamic acid, cysteine and glycine, increases the physiological stability and agglomeration resistance of the NPs to certain proteins. In addition, polymers, such as polyethylene glycol (PEG), [21,29] also increase the physiological stability and can be used as coordination sites for biomolecules.…”

Section: Introductionmentioning

confidence: 99%

Biocompatible superparamagnetic nanoparticles with ibuprofen as potential drug carriers

et al. 2020

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This article describes the synthesis, characterization, in vitro cytotoxic and genotoxicity evaluation of chitosan-iron oxide nanoparticles (Fe 3 O 4 -CS) as vehicles for ibuprofen (IBU) molecule. Magnetite (Fe 3 O 4 ) nanoparticles were synthesized by co-precipitation of iron salts and coated with chitosan, leading to the formation of Fe 3 O 4 -CS hybrid nanoparticles, and then IBU was adsorbed on the surface of the modified nanoparticles. The physicochemical, morphological, and magnetic properties of the nanoparticles were determined by X-ray powder diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and SQUID magnetic measurements. The nanoparticles have shown stability in aqueous medium presenting an average hydrodynamic size of 36 ± 9 nm for Fe 3 O 4 -CS, and 132 ± 1 nm for Fe 3 O 4 -CS-IBU. The cytotoxicity of nanoparticles using the cell viability of the blood mononuclear cell fraction and the analysis of gene expression of the repair genes hMSH2, hMSH6 and NF-kB were performed to evaluate their applicability as drug carriers. The results showed that Fe 3 O 4 -CS nanoparticles are suitable candidates as magnetic vehicles for ibuprofen in biomedical applications. Graphic abstract

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Synthesis, characterization, and cytotoxicity of glutathione-PEG-iron oxide magnetic nanoparticles

Cited by 19 publications

References 70 publications

Superparamagnetic iron oxide nanoparticles dispersed in Pluronic F127 hydrogel: potential uses in topical applications

Superparamagnetic iron oxide nanoparticles dispersed in Pluronic F127 hydrogel: potential uses in topical applications

Mitochondrial Dysfunction in Cardiorenal Syndrome 3: Renocardiac Effect of Vitamin C

Biocompatible superparamagnetic nanoparticles with ibuprofen as potential drug carriers

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