Synthesis, Characterization and Cytotoxic Activity of Zn(ii) Cysteine Dithiocarbamate in Breast Cancer (Mcf-7)

Abstract

To synthesize and characterize complex compound of Zn(II) with cysteine dithiocarbamate ligands and studies its cytotoxic activity in breast cancer (MCF-7). The complex of Zn(II) cysteine dithiocarbamate was prepared by "in situ method" and characterized by using Ultraviolet-Visible (UV-Vis) and Infra-Red (IR) spectroscopy, melting point and conductivity. The results of UV-Vis maximum spectrums of Zn(II)cysteine dithiocarbamate at 282 nm and 374 nm indicated that electronic transition π→π* dan n→π* of CS2 and N=C=S. While the presence of IR spectra at the wavelength in the region of 397-534 cm-1 indicated that has been coordination occurred between Zn(II) with Sulphur (S), Nitrogen (N) and Oxygen(O) atoms from cysteine dithiocarbamate ligands. Complex characterization using UV-Vis and IR showed that complex Zn(II) cysteine dithiocarbamate compounds were successfully synthesized by the in-situ method. The results of the cytotoxicity assay of Zn(II) cysteine dithiocarbamate complex compounds in MCF-7 cells obtained IC50 values of Zn(II) complex = 98.60 μg/mL and cisplatin (IC50 = 200 μg/mL. These result can be seen that IC50 value of Zn(II) complex is lower than cisplatin so that Zn(II) complex has very good inhibitory activity against MCF-7 cancer cells compared to cisplatin and it can even cause apoptosis in cancer cells.

Review on Anticancer Activity of Essential Metal Dithiocarbamate Complexes

“…The cytotoxicity of the Zn(II) 1 complex on MCF-7 cells was assessed using the MTS assay (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy phenyl)-2-(4-sulfophenyl)-2H-tetrazolium) was observed for 24 h. The MTS assay is one of the most commonly used assays to assess cell proliferation and cytotoxicity [56]. Because the Zn complex's IC 50 value is lower than that of cisplatin, it has higher cytotoxicity than cisplatin in inducing morphology in MCF-7 cancer cells [17].…”

“…Ni complexes with oxoaporfin derivatives have very high cytotoxicity values against tumor cells [15]. Zn is the second-most common essential transition metal ion in the human body whereas DNA can covalently attach to Zn's radius [16][17]. Zn plays a part in the development, differentiation, and demise of cells, among other biological processes.…”

Novel Complex of Zinc (II) Dichloroethylenediamine: Synthesis, Characterization, In-silico, and In-vitro Evaluation against Cervical Cancer Cells

“…Among the metal complexes, zinc (II) species was the most potent against all bacterial strains, with Zinc (II) derivatives presenting more significant activity than ampicillin and fluconazole (Yeo et al, 2021). Zn (II) cysteine dithiocarbamate covalently binds with DNA from cancer cells, leading to apoptosis (Irfandi and Prihantono, 2019). This complex was reported by Kartina et al, 2019 to be effective against mycobacterium tuberculosis and cancer cells.…”