Synthesis, Characterization, and Antimicrobial Activity of Nano-Hydroxyapatite-Zinc for Bone Tissue Engineering Applications

Abstract

The bone implants used in tissue repair are susceptible to infections caused by staphylococci, specifically Staphylococcus aureus. Hence, the development of better biological materials that provide antimicrobial activity in bone tissue engineering is required. The nanoparticles of hydroxyapatite (nHAp) and nHAp dopped with Zn (nHAp-Zn) were prepared by the wet chemical method and the ion exchange method, respectively. They were characterized using SEM, AFM, FTIR and XRD. The antibacterial activity of nHAp and nHAp-Zn was determined with Gram-negative and Gram-positive bacterial strains. The results indicated that nHAp alone was acting as an inert matrix and when substituted with Zn, it showed better antibacterial activity. The nHAp-Zn was found to be non-toxic to osteoprogenitor cells. Thus, due to the antimicrobial property of nHAp-Zn nanoparticles, we suggest that they would have potential applications towards bone tissue engineering.

Biocompatibility and Anti-Bacterial Activity of Zn-Containing HA/TiO2 Hybrid Coatings on Ti Substrate

“…It can also promote bone metabolism, growth, mineralization, increase bone density and prevent bone resorption 6) . Researches confirmed that the calcium phosphate ceramic coatings containing zinc promoted biological activity of osteoblasts than the pure HA coating in vitro 7) . It can also be used as antimicrobial elements, and showed good anti-bacterial activity 8).…”

Substituted hydroxyapatites for biomedical applications: A review

“…only as a bone-forming agent [10], but also as a microbial agent [11,12], as a drug delivery system [13], as a coating [14] and as a biomarker for nanomedical applications [15,16].…”

Multifunctional and hybrid systems based on calcium-phosphate and hydroxyapatite for preventive and regenerative medicine

“…Modifikacija HAp-a dopiranjem ili supstitucijom anjona ili katjona proširuje potencijalnu biomedicinsku primenu ovog materijala. Metal-dopirani HAp pokazao je veliki osteogenetski potencijal [22], takođe i antimikrobna svojstva [23], a može biti pogodan i za dobijanje prevlaka [24], sistema za dostavu medikamenata [25], biomarkera za nanomedicinske aplikacije [26], itd. Katjon poput Ca 2+ i anjoni poput PO4 3-i OH -jona, iz strukture HAp-a, se mogu delimično ili potpuno zameniti drugim jonima, bez narušavanja kristalografske simetrije.…”