Synthesis, characterization and antifungal activity of chemically and fungal-produced silver nanoparticles against <i>Trichophyton rubrum</i>

Pereira, Leonel; Dias, Nicolina; Carvalho, José Murilo de; Fernandes, Sara; Santos, Cledir; Lima, Nelson

doi:10.1111/jam.12652

Cited by 107 publications

(58 citation statements)

References 69 publications

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“…Antifungal activity against Cladosporium cladosporioides, Aspergillus niger [109], Trichophyton rubrum [104] and Candida sp. [93] has been demonstrated.…”

Section: Toxic Effects Of Agnps In Microorganismsmentioning

confidence: 99%

Toxic effects of silver nanoparticles in mammals – does a risk of neurotoxicity exist?

Skalska¹,

Strużyńska²

2015

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A b s t r a c t Over the last decade, silver nanoparticles have become an important class of nanomaterials utilized in the development of new nanotechnologies. Despite the fact that nanosilver is used in many commercial applications, our knowledge about its associated risks is incomplete. Although a number of studies have been undertaken to better understand the impact of silver nanoparticles on the environment, aquatic organisms and cell lines, little is known about their side effects in mammalian organisms. This review summarizes relevant data and the current state of knowledge regarding toxicity of silver nanoparticles in mammals, as well as the accumulated evidence for potent neurotoxic effects. The influence of nanosilver on the central nervous system is significant because of evidence indicating that it accumulates in mammalian brain tissue.

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“…Antifungal activity against Cladosporium cladosporioides, Aspergillus niger [109], Trichophyton rubrum [104] and Candida sp. [93] has been demonstrated.…”

Section: Toxic Effects Of Agnps In Microorganismsmentioning

confidence: 99%

Toxic effects of silver nanoparticles in mammals – does a risk of neurotoxicity exist?

Skalska¹,

Strużyńska²

2015

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“…A study on synthesis of AgNPs from C. albicans with antifungal activity against C. albicans has also been reported (Astuti et al 2017). Chemically synthesized AgNPs exhibits low MIC values along with high toxicity and low stability (Pal et al 2007;Kittler et al 2010;Pereira et al, 2014). Recently, anticandidal activity of rod-shaped silver loaded hydroxyapatite nanocomposites has been reported with MIC of 125 µg ml À1 against C. albicans (Gottardo et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Acinetobacter sp. mediated synthesis of AgNPs, its optimization, characterization and synergistic antifungal activity against C. albicans

Nadhe

Singh²,

Wadhwani

et al. 2019

J of Applied Microbiology

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Aims To synthesize silver nanoparticles (AgNPs) with cell free extract of Acinetobacter sp. and evaluate antifungal activity against planktonic and biofilm of Candida. Also, to study mechanism of antifungal action of AgNPs. Methods and Result Acinetobacter spp were screened for synthesis of AgNPs. Physio‐chemical parameters were optimized to obtained monodispersed nanoparticles. Optimized nanoparticles were characterized using spectroscopic, microscopic and diffraction techniques. Antifungal and biofilm disruption activity of AgNPs (10 ± 5 nm) were investigated against C. albicans. Mechanism of antifungal activity of nanosilver was deduced by growth curve, reactive oxygen species generation, thiol interaction and microscopic analysis. Acinetobacter sp. GWRFH 45 gave maximum synthesis of AgNPs. At optimized condition monodispersed, spherical nanoparticles were obtained which were crystalline with negative surface charge. AgNPs exhibited antifungal activity against planktonic cells and biofilm of Candida. AgNPs showed synergistic effect with amphotericin B as well as fluconazole against biofilm disruption. AgNPs were found to affect growth of Candida, generate reactive oxygen species and disrupt cellular morphology. Conclusions Cell free extract of A. calcoaceticus GWRFH 45 has ability to synthesize AgNPs. AgNPs alone and in combination with drugs have potential to inhibit C. albicans. Significance and Impact of the Study This is the first report of bacteriogenic AgNPs used in combination with antifungal drugs against Candida.

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“…Nature provides a rich source of various biological diversity including plants and microorganisms, which shows the ability to synthesize nanoparticles. Among several biological species; various plant extracts, bacteria, algae and fungi have been studied for this action (Elavazhagan and Arunachalam 2011, Govindaraju et al 2009, Pereira et al 2014, Singh et al 2014, 2015a, 2015b, Wang et al 2015. This green route of synthesis minimizes the side effects of chemical and physical methods by avoiding the utilization of toxic chemicals and creating hazardous by products.…”

Section: Introductionmentioning

confidence: 99%

A strategic approach for rapid synthesis of gold and silver nanoparticles byPanax ginsengleaves

Singh

Kim

Yang

2015

Artificial Cells, Nanomedicine, and Biotechnology

182

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The study highlights the synthesis of gold nanoparticles and silver nanoparticles by fresh leaves of Panax ginseng, an herbal medicinal plant. The reduction of auric chloride and silver nitrate led to the formation of gold and silver nanoparticles within 3 and 45 min, at 80°C, respectively. The developed methodology was rapid, facile, ecofriendly and the utmost significant is quite economical, which did not require subsequent processing for reduction or stabilization of nanoparticles. The nanoparticles were further characterized by Ultraviolet-visible spectroscopy (UV-vis) which showed the relevant peak for gold and silver nanoparticles at 578 and 420 nm, correspondingly. Field-emission transmission electron microscopy (FE-TEM) displayed the spherical shape of monodispersed nanoparticles. FE-TEM revealed that the gold nanoparticles were 10-20 nm and silver nanoparticles were 5-15 nm. The energy dispersive X-ray (EDX) and elemental mapping results indicated the maximum distribution of gold and silver elements in the respective nanoproducts, which further corresponds the purity. Further, the X-ray diffraction (XRD) results confirm the crystalline nature of synthesized nanoparticles. The biosynthesized AgNPs served as an efﬁcient antimicrobial agent at 3 μg concentration against many pathogenic strains for instance, Escherichia coli, Salmonella enterica, Vibrio parahaemolyticus, Staphylococcus aureus, Bacillus anthracis and Bacillus cereus. In addition, AgNPs showed complete inhibition of biofilm formation by S. aureus and Pseudomonas aeruginosa at 4 μg/ml concentration. Moreover, the AuNPs and AgNPs found as a potent anticoagulant agent. Thus, the study claims the rapid synthesis of gold and silver nanoparticles by fresh P. ginseng leaf extract and its biological applications.

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Synthesis, characterization and antifungal activity of chemically and fungal-produced silver nanoparticles against Trichophyton rubrum

Abstract: Bio-AgNPs could be used as antifungal agents, namely against dermatophytes.

Cited by 107 publications

References 69 publications

Toxic effects of silver nanoparticles in mammals – does a risk of neurotoxicity exist?

Toxic effects of silver nanoparticles in mammals – does a risk of neurotoxicity exist?

Acinetobacter sp. mediated synthesis of AgNPs, its optimization, characterization and synergistic antifungal activity against C. albicans

A strategic approach for rapid synthesis of gold and silver nanoparticles byPanax ginsengleaves

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