Background:
Searching for new cytotoxic agents with apoptosis induction may represent a viable strategy for
cancer treatment to overcome the increased resistance to available anticancer agents.
Objective:
The purpose of the current study aimed at preparation and anticancer evaluation of two new series of 2Hquinolinone and halogenated 2H-quinolinone derivatives against two cancer cell lines. Methods: Two new series of 2Hquinolinone and halogenated 2H-quinolinone derivatives were prepared and screened for their cytotoxicity against breast
MCF-7 and liver HepG-2 cancer cell lines as well as normal breast MCF-10a.
Results:
The tested molecules revealed good cytotoxicity and selectivity toward cancer cell line relative to normal cells.
These compounds were analyzed by DNA flow cytometry on MCF-7 cells. They were found to cause G2/M phase arrest
and induced apoptosis at pre-G1 phase. In addition, increased caspase 3/7 activity and decreased osteopontin expression
verified the apoptotic activity.
Conclusion:
The potent compounds discovered in this study can be a hit for discovery of new cytotoxic agents and are
worthy of further investigation.