Synthesis and stability of developmentally regulated dictyostelium mRNAs are affected by cell-cell contact and cAMP

Chung, Sungwon; Landfear, Scott M.; Blumberg, Daphne D.; Cohen, Naomi; Lodish, Harvey F.

doi:10.1016/0092-8674(81)90104-5

Cited by 208 publications

(148 citation statements)

References 28 publications

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“…3). A previous report showed that this clone contains only one gene (5). This is probably due to the fact that it was analyzed in a glyoxal gel, where the RNA was denatured by glyoxal, and was run in an agarose-acrylamide composite gel.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, it is likely that the hypersensitive sites associated with these genes may be present at all stages of development. We chose to study a clone called SC253, which contains a gene that is induced only at the multicellular stage, and as a control, a clone called SC29, which contains three genes that are expressed in vegetative cells as well as at all stages of development (5).…”

Section: Resultsmentioning

confidence: 99%

“…D. discoideum AX3 was used for these experiments (15). Conditions for the axenic growth and development of this strain have been described previously (1,3,5).…”

Section: Methodsmentioning

confidence: 99%

“…Pulsatile addition of cyclic AMP to a starved cell suspension can mimic cell aggregation and induce some but not all of these cell type-specific genes (4), indicating that cyclic AMP is one of the messengers relaying signals between cells to direct differentiation. Some postaggregation specific genes can only be induced by tight cell-cell contact (4,5), indicating that interactions between cell surface molecules or other as yet unidentified diffusible molecules may serve as signals to regulate transcription of these genes.…”

mentioning

confidence: 99%

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Developmental regulation of DNase I-hypersensitive sites in Dictyostelium discoideum.

Ayres¹,

Neuman²,

Rowekamp³

et al. 1987

Mol. Cell. Biol.

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We have studied two regions of Dictyostelium discoideum chromatin and identified several DNase I-hypersensitive sites in these regions. One of these sites is located about 300 to 500 bases upstream of the transcriptional start site of a gene that is expressed at all stages of development. This site is present in both vegetative cells and postaggregation cells. Another hypersensitive site is associated with a gene that is expressed only after the multicellular stage. This site is located about 400 bases upstream of the start site, and it is present only in postaggregation cells. Thus, much like higher eucaryotes, D. discoideum contains DNase I-hypersensitive sites that may be involved in the regulation of the genes with which they are associated.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…D. discoideum AX3 was used for these experiments (15). Conditions for the axenic growth and development of this strain have been described previously (1,3,5).…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Developmental regulation of DNase I-hypersensitive sites in Dictyostelium discoideum.

Ayres¹,

Neuman²,

Rowekamp³

et al. 1987

Mol. Cell. Biol.

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“…Analysis of the complexity and diversity of the mRNA populations throughout development has shown that polyribosomes of late aggregating cells contain 2500 mRNA species that are absent from growing cells (4,5). Appearance of these mRNAs is regulated at the transcriptional level (6,7). The coordinated control (and expression) of genes that are expressed at the same time during development seems logically to require the existence ofcommon elements for their regulation.…”

mentioning

confidence: 99%

Repetitive DNA sequences cotranscribed with developmentally regulated Dictyostelium discoideum mRNAs.

Zuker¹,

Lodish²

1981

Proc. Natl. Acad. Sci. U.S.A.

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We have isolated a clone of Dictyostelium discoileum genomic DNA, pB41-6, that contains sequences that are reiterated and interspersed in the genome. The expression of these reiterated sequences is developmentally regulated, and they appear to be linked only to developmentally regulated mRNAs. We have used clone pB41-6 to isolate a set of complementary cDNA clones. Analysis of two selected clones indicates that they encode developmentally regulated mRNA species and, by means of the repetitive elements they contain, hybridize to many developmentally regulated mRNAs. In addition, we have defined two families ofmRNA species carrying these repetitive sequences: one is induced at 5.5 hr of development and the other is induced between 5.5 and 15 hr ofdevelopment. Since most ofthe mRNA species that have sequences complementary to clone pB41-6 accumulate in a coordinated fashion during development, these sequences may play an important regulatory role in the developmental program of Dictyoatelium.

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Regulation of messenger RNA stability in eukaryotic cells

1987

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Regulation of the cytoplasmic stability of mRNAs has recetly been identified as a major control mechanism which governs mRNA levels in a variety of eukaryotic systems. In this review we discuss what is known about several experimental systems that exhibit regulated mRNA stability, describe the mechanisms that cells may use to achieve control of mRNA degradation, and suggest areas of future investigation likely to provide new insights into this process.

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Synthesis and stability of developmentally regulated dictyostelium mRNAs are affected by cell-cell contact and cAMP

Cited by 208 publications

References 28 publications

Developmental regulation of DNase I-hypersensitive sites in Dictyostelium discoideum.

Developmental regulation of DNase I-hypersensitive sites in Dictyostelium discoideum.

Repetitive DNA sequences cotranscribed with developmentally regulated Dictyostelium discoideum mRNAs.

Regulation of messenger RNA stability in eukaryotic cells

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