Developmental regulation of DNase I-hypersensitive sites in Dictyostelium discoideum.

Ayres, K; Neuman, W; Rowekamp, W G; Chung, S

doi:10.1128/mcb.7.5.1823

Cited by 6 publications

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another circumstantial piece of evidence for such a role comes from the ubiquity of GSE-like sequences. They are found upstream of vegetatively transcribed actin genes (20), the cAMP-repressible discoidin I genes (21,22) and the DIF-inducible pDd56 gene (23,24). It will obviously be necessary to prove the functional identity of these sequences but, assuming this to be the case, then these elements would appear to be playing a role in Dictyostelium gene transcription analagous to that played by promoter-elements of higher eukaryotes such as "CCAAT" box or the SP1 binding site.…”

Section: Discussionmentioning

confidence: 99%

Multiple copies of a G-rich element upstream of a cAMP-inducible Dictyostelium gene are necessary but not sufficient for efficient gene expression

Pears¹,

Williams²

1988

Nucl Acids Res

View full text Add to dashboard Cite

The cysteine proteinase 1 (CP1) and cysteine proteinase 2 (CP2) genes of Dictyostelium discoideum encode co-ordinately expressed mRNA sequences which are inducible by extracellular cAMP. There are short, G-rich sequence elements upstream of both genes and we have previously shown that deletion of these elements from the CP2 gene abolishes cAMP-inducibility. We show here that the G-rich element from the CP1 gene is functionally homologous to that in the CP2 gene by reconstituting cAMP-inducibility in a deletion mutant of the CP2 gene using CP1-derived sequences. Both the CP1 and CP2 genes contain multiple G-rich elements. We show that efficient induction requires at least two copies of the CP1 element and that their relative orientation is unimportant. Two copies of an inverted relative orientation are, however, inactive when moved upstream of their normal position and are incapable of conferring cAMP-inducibility on a heterologous gene. These observations suggest that these sequences are either essential promoter elements, not themselves interacting with the inducer, or that their interaction with a separate class of control sequences is necessary for inducible expression.

show abstract

Section: Discussionmentioning

confidence: 99%

Multiple copies of a G-rich element upstream of a cAMP-inducible Dictyostelium gene are necessary but not sufficient for efficient gene expression

Pears¹,

Williams²

1988

Nucl Acids Res

View full text Add to dashboard Cite

show abstract

“…A DNase I-hypersensitive site that increases with gene activity has also been observed upstream of a developmentally regulated gene (SC253) by Ayres et al (1). The appearance of the site was correlated with the activity of the gene.…”

Section: Resultsmentioning

confidence: 72%

Identification of a signal transduction response sequence element necessary for induction of a Dictyostelium discoideum gene by extracellular cyclic AMP.

Pavlovic¹,

Haribabu²,

Dottin³

1989

Mol. Cell. Biol.

View full text Add to dashboard Cite

The signal transduction pathways that lead to gene induction are being intensively investigated in Dictyostelium discoideum. We have identified by deletion and transformation analysis a sequence element necessary for induction of a gene coding for uridine diphosphoglucose pyrophosphorylase (UDPGP1) of D. discoideum in response to extracellular cyclic AMP (cAMP). This regulatory element is located 380 base pairs upstream of the transcription start site and contains a G+C-rich partially palindromic sequence. It is not required for transcription per se but is required for induction of the gene in response to the stimulus of extracellular cAMP. The cAMP response sequence is also required for induction of the gene during normal development. A second A+T-rich cis-acting region located immediately downstream of the cAMP response sequence appears to be essential for the basal level of expression of the UDPGP1 gene. The position of the cAMP response element coincides with a DNase I-hypersensitive site that is observed when the UDPGP1 gene is actively transcribed.

show abstract

Characterization of two divergently transcribed Dictyostelium gene pairs and identification of G‐rich sequence element lying between them with the characteristics of a basal promoter element

Driscoll¹,

Pears²,

Williams³

1988

Dev. Genet.

View full text Add to dashboard Cite

The cysteine proteinase 1 (CP1) and cysteine proteinase 2 (CP2) genes of Dictyostelium discoideum encode coordinately expressed mRNA sequences that are inducible by extracellular cAMP. Both genes form part of divergently transcribed gene pairs. The gene proximal to CP1 is coordinately regulated and encodes a protein containing several potential zinc binding domains of the kind found in DNA binding proteins. The gene proximal to CP2 is a constitutively transcribed gene of unknown function. There are multiple, short, G-rich sequence elements between both gene pairs, and deletion of the pair of elements 200 nucleotides upstream from the CP2 gene abolishes cAMP-inducibility. A synthetic oligonucleotide, containing two copies of the G-rich element from the CP1 gene, will reconstitute cAMP-inducibility in the deletion mutant of the CP2 gene. This shows that the elements in the two genes are functionally homologous. Efficient induction requires at least two copies of the CP1 element, but their relative orientation is unimportant. Two copies in an inverted orientation are, however, inactive when moved upstream of their normal position and are incapable of conferring cAMP-inducibility on a heterologous gene. These observations suggest that these sequences are either essential promoter elements, not themselves interacting with the inducer, or that their interaction with a separate class of control sequences is necessary for inducible expression.

show abstract

Developmental regulation of DNase I-hypersensitive sites in Dictyostelium discoideum.

Cited by 6 publications

References 22 publications

Multiple copies of a G-rich element upstream of a cAMP-inducible Dictyostelium gene are necessary but not sufficient for efficient gene expression

Multiple copies of a G-rich element upstream of a cAMP-inducible Dictyostelium gene are necessary but not sufficient for efficient gene expression

Identification of a signal transduction response sequence element necessary for induction of a Dictyostelium discoideum gene by extracellular cyclic AMP.

Characterization of two divergently transcribed Dictyostelium gene pairs and identification of G‐rich sequence element lying between them with the characteristics of a basal promoter element

Contact Info

Product

Resources

About