Identification of a signal transduction response sequence element necessary for induction of a Dictyostelium discoideum gene by extracellular cyclic AMP.

Pavlovic, Jovan; Haribabu, Bodduluri; Dottin, Robert P.

doi:10.1128/mcb.9.11.4660

Cited by 27 publications

(12 citation statements)

References 41 publications

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“…Although all of these genes have been shown to be transcriptionally induced by cAMP and show similar developmental kinetics of expression, the only apparent similarity between the putative regulatory elements is that they are G/C rich (Williams et al 1980;Mehdy et al 1983;Mehdy and Firtel 1985;Pears et al 1985;Williams et al 1985;Datta et al 1986;Driscoll and Williams 1987;Pavlovic et al 1989). The rest of the 5'-flanking regions are >90% A/T, similar to the majority of the 5'-flanking regions of other Dictyostelium genes (Kimmel and Firtel 1983).…”

Section: Structure Of the G/c-rich Elementsmentioning

confidence: 84%

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A developmentally regulated trans-acting factor recognizes dissimilar G/C-rich elements controlling a class of cAMP-inducible Dictyostelium genes.

Hjorth¹,

Pears²,

Williams³

et al. 1990

Genes Dev.

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Transcriptional response elements involved in the cAMP-inducible and developmentally regulated expression of the Dictyostelium aggregate-stage gene pst-cath/CP2 have been shown to include a G/C-rich sequence element [G-box regulatory element {GBRE1]. We have recently identified a trans-acting factor, GBF (GBRE binding factor), that specifically interacts with this sequence and have shown that the binding activity of GBF to GBRE is developmentally regulated and inducible by cAMP. Here, we examine further the possible role of GBF in the regulation of pst-cath/CP2 and three other coordinately regulated, cAMP-inducible aggregate-stage genes. We show that GBF itself {or other closely related factorsl recognizes dissimilar G/C-rich elements present in the 5'-flanking regions of these genes and that the ability of the individual, distinct G/C-rich elements to confer regulated expression on a promoter deletion mutant of the pst-cath/CP2 gene is correlated with the relative affinity for GBF. G/C-rich elements carrying point mutations that prevent in vitro binding of GBF to two of the G/C-rich elements fail to activate expression in vivo. An analysis of major points of contact between the GBF protein and two distinctly different binding sites suggests that binding of GBF to these sequence elements involves a considerable degree of flexibility in DNA-protein interactions. These results suggest that the regulated expression of a class of aggregate-stage cAMP-inducible genes involves the interaction of GBF or homologous factors with dissimilar G/C-rich sequence elements and that induction of GBF activity or that of homologous factors by cAMP may thus be a limiting step in the induction of this temporally coordinate set of genes during Dictyostelium development. Dictyostelium discoideum propagates as a vegetative amoeba in the presence of nutrients and initiates, upon starvation, a multicellular differentiation process that results in the formation of a mature fruiting body containing two distinctly different cell types--stalk cells and spores. Within several hours of starvation, Dictyostelium cells aggregate in response to pulsatile cAMP signals. Binding of cAMP to cell-surface receptors activates adenylate cyclase, and repeated cycles of cAMP synthesis and secretion serve to transmit, or relay, the chemotactic signal outward through the population of aggregating cells (see Janssens and van Haastert 1987;Firtel et al. 1989). After formation of tightly associated multicellular aggregates, a migrating pseudoplasmodium (slug) is formed in which precursor cells to the 3Corresponding author.

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Section: Structure Of the G/c-rich Elementsmentioning

confidence: 84%

“…Recent data definitely show that this element is required for cAMP-induced expression (Pavlovic et al 1989). …”

Section: Structure Of the G/c-rich Elementsmentioning

confidence: 99%

A developmentally regulated trans-acting factor recognizes dissimilar G/C-rich elements controlling a class of cAMP-inducible Dictyostelium genes.

Hjorth¹,

Pears²,

Williams³

et al. 1990

Genes Dev.

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“…The promoters of many late genes contain CA/GTrich sequences that have been shown to be essential for their activation during development or in response to cAMP [Haberstroh and Firtel 1990;Haberstroh et al 1991 (SP60]; Fosnaugh and Loomis 1993 (SP70) ;PowellCoffman and Firtel 1994 [PspB], Ceccarelli et al 1991Ceccarelli et al , 1992; Early et al 1993 [ecmA); Firtel 1987, 1988;Pears and Williams 1987 {pst-cathepsin/ CP2); Pavlovic et al 1989 {UDPGP]} Esch et al 1992 [DdiasD]]. The G-box binding factor, or GBF, is a developmentally regulated activity that binds specifically to a number of CA/GT-rich late gene regulatory elements [Hjorth et al 1989 {CP2 and DdiasD]-, Hjorth et al 1990 [UDPGP); CeccareUi et al 1992 [ecmB]-, J.…”

Section: Conesponding Authormentioning

confidence: 99%

Cloning and characterization of the G-box binding factor, an essential component of the developmental switch between early and late development in Dictyostelium.

Schnitzler

Fischer²,

Firtel³

1994

Genes Dev.

113

120

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During Dictyostelium development, the cAMP-regulated induction of cell-type-speciKc late genes marks a developmental switch from the initial formation of the multicellular organism to the differentiation of the various cell types that mediate morphogenesis and eventually give rise to the mature fruting body. The G-box binding factor (GBF) is a developmentally regulated Dictyostelium transcription factor whose affinity for a DNA sequence correlates with the ability of that sequence to confer inducibility to late gene promoters in response to high, continuous levels of extracellular cAMP. We report the purification of GBF and cloning of the gene that encodes it, as confirmed by in vitro production of GBF activity. The predicted protein is highly basic and contains two putative zinc fingers. Disruption of the GBF gene by homologous recombination results in the loss of all GBF DNA-binding activity, developmental arrest at the loose aggregate stage, and the loss of late gene induction during development or in response to extracellular cAMP. Constitutive expression of GBF complements the null phenotype and allows for the rapid activation of a class of late genes in response to cAMP. Our results indicate that GBF acts as an extracellular cAMP-responsive transcriptional activator regulating late gene expression and is an essential component of a developmental switch between aggregation and cellular morphogenesis.

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“…Vegetative Cells-Deletion analysis of the rnrB promoter suggests that the region located between Ϫ212 and Ϫ130, with an A/T content of 96%, is required for expression in vegetative (40,(42)(43)(44)(45)(46). Moreover, the A/T-rich upstream activating sequence of actin 15 when placed in a developmentally regulated promoter can direct expression in vegetative cells (42).…”

Section: Cis-regulatory Element Responsible For Rnrb Expression Inmentioning

confidence: 99%

Identification of cis-Regulating Elements andtrans-Acting Factors Regulating the Expression of the Gene Encoding the Small Subunit of Ribonucleotide Reductase inDictyostelium discoideum

Bonfils¹,

Gaudet

Tsang

1999

Journal of Biological Chemistry

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We have examined the promoter of rnrB, the gene encoding the small subunit of ribonucleotide reductase of Dictyostelium discoideum, using lacZ as a reporter gene. Deletion analysis showed that expression of this gene in vegetative cells involves an A/T-rich element, whereas its expression in prespore cells during development requires a region encompassing two G/C-rich elements, designated box A and box B. Removal of boxes A and B results in very low level of activity. When either box A or box B is deleted, prestalk cells adjacent to the prespore zone also express ␤-galactosidase. The behavior of these cis-regulatory elements implies that the mechanism regulating the prespore-specific expression of rnrB is different from that regulating other known prespore genes.We have used electrophoretic mobility shift assays to identify factors that interact with box A and box B. Box A interacts with a factor that is found in the nuclear fraction. While box B interacts with a factor that is present in the cytosolic fraction throughout growth and development, its presence in the nuclear fraction is developmentally regulated. Results from competition assays suggest that both box A and box B interact with transcriptional activators that have not been characterized previously.

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Identification of a signal transduction response sequence element necessary for induction of a Dictyostelium discoideum gene by extracellular cyclic AMP.

Cited by 27 publications

References 41 publications

A developmentally regulated trans-acting factor recognizes dissimilar G/C-rich elements controlling a class of cAMP-inducible Dictyostelium genes.

A developmentally regulated trans-acting factor recognizes dissimilar G/C-rich elements controlling a class of cAMP-inducible Dictyostelium genes.

Cloning and characterization of the G-box binding factor, an essential component of the developmental switch between early and late development in Dictyostelium.

Identification of cis-Regulating Elements andtrans-Acting Factors Regulating the Expression of the Gene Encoding the Small Subunit of Ribonucleotide Reductase inDictyostelium discoideum

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