Synthesis and in Vitro Cytocompatibility of Segmented Poly(Ester-Urethane)s and Poly(Ester-Urea-Urethane)s for Bone Tissue Engineering

Abstract

Two series of segmented polyurethanes were obtained and their mechanical and thermal properties as well as their biodegradability and cytotoxicity were evaluated. The chemical nature of the polyurethanes was varied by using either 1,4 butanediol (poly-ester-urethanes, PEUs) or l-lysine ethyl ester dihydrochloride (poly-ester-urea-urethanes, PEUUs) as chain extenders. Results showed that varying the hard segment influenced the thermal and mechanical properties of the obtained polymers. PEUs showed strain and hardness values of about 10–20 MPa and 10–65 MPa, respectively. These values were higher than the obtained values for the PEUUs due to the phase segregation and the higher crystallinity observed for the polyester-urethanes (PEUs); phase segregation was also observed and analyzed by XRD and DSC. Moreover, both series of polymers showed hydrolytic degradation when they were submerged in PBS until 90 days with 20% of weight loss. In vitro tests using a Human Osteoblastic cell line (Hob) showed an average of 80% of cell viability and good adhesion for both series of polymers.

Biological evaluation of the modified nano-amorphous phosphate calcium doped with citrate/poly-amino acid composite as a potential candidate for bone repair and reconstruction

“…Biocompatibility is a critical element in the evaluation of a biomaterial [ 32 , 33 ]. The cell viabilities result (Fig.…”

Preparation, Characterization and In Vitro Biological Evaluation of a Novel Pearl Powder/Poly-Amino Acid Composite as a Potential Substitute for Bone Repair and Reconstruction

“…Biocompatibility is a critical element in the evaluation of a biomaterial which determines the final destination of the biomaterials [5,41]. There are various factors influencing the biocompatibility, such as pH and the product of degradation.…”

Physical properties and dielectric behavior of the poly(urethane‐urea) based on o‐dianisidine and renewable cross‐linkers

“…16 PCL is one of the most extensively used biodegradable polyesters, due to the fact that the degradation product of PCL is 6-hydroxyhexanoic acid, which is naturally metabolized by the human body. 17 o-Dianisidine (3,3 0 -dimethoxybenzidine) we used it in these syntheses as chain extender. Until now, odianisidine was used to obtain soluble polyphenol derivatives containing azomethine groups, 18 conjugated aromatic polyazomethine, 19 poly(azomethine-urethane), 20 in synthesis of phenol Schiff bases, 21 as accelerator in the curing process of benzoxazine based bisphenol Aaniline, 22 in synthesis of poly (keto-amine).…”