Synthesis and in vitro antifungal efficacy of Cu–chitosan nanoparticles against pathogenic fungi of tomato

Assessment of Cu- Chitosan Nanoparticles for its Antibacterial Activity against Pseudomonas syringae pv. glycinea

“…For synthesis of Cu-chitosan NPs, a wellestablished and reproducible method of cross linking coupled with ultra-sonication was used as described earlier (Qi et al, 2004;Du et al, 2009;Corradini et al, 2010;Fan et al, 2012;Saharan et al, 2013;Saharan et al, 2015;Manikandan and Sathiyabama, 2016;Choudhary et al, 2017). 0.1 gm of chitosan (low molecular weight and 80% Ndeacetylation, Sigma-Aldrich, St.Louis, USA) mixed in 100 ml of 1% acetic acid and stirrer at 500-550 rpm(for 30 minutes).…”

“…Cu-chitosan NPs were characterized for mean size, size distribution, functional group analysis and surface morphology by particle size analyzer (DLS: Dynamic Light Scattering), Fourier Transform Infra Red (FTIR), Transmission electron microscopy (TEM) and Scanning electron microscopy (SEM) through standardized methods (Saharan et al, 2013;Saharan et al, 2015). Elemental analysis of Cu-chitosan NPs was gauged by energy dispersive spectroscopy (SEM-EDS).…”

“…Cu-chitosan NPs was prepared by interaction of TPP anion with cationic chitosan and further chelating of copper ions using ionic gelation method (Jaiswal et al, 2012;Saharan et al, 2015). Chitosan has inter and intramolecular hydrogen bonding.…”

“…Cu-chitosan NPs prepared in the present study exhibit a white crystal powder and appeared a semitransparent colloidal in aqueous. Cu-chitosan NPs were prepared by the interaction of oppositely charged macromolecules (chitosan and TPP) using ionic gelation method (Qi et al, 2004;Du et al, 2009;Corradini et al, 2010;Fan et al, 2012;Saharan et al, 2013;Saharan et al, 2015;Manikandan and Sathiyabama, 2016).…”

“…Hence, Cu-chitosan complexes may serve as a reservoir for the slow release of Cu ions. The copper blending with chitosan have been developed to improve efficacy of their antibacterial (Qi et al, 2004) and antifungal activities (Brunel et al, 2013;Saharan et al, 2013;Saharan et al, 2015).…”