Synthesis and identification of artificial antigens for cadmium and copper

Kong, Tao; Liu, G.W.; Li, X.B.; Wang, Z.; Zhang, Z.G.; Xie, Guanghong; Zhang, Yongzheng; Sun, Jinru; Xu, Chuanlai

doi:10.1016/j.foodchem.2010.05.087

Cited by 21 publications

(7 citation statements)

References 14 publications

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“…The concentration of protein conjugate was determined by Pierce BCA protein assay. The amount of Cr of the complete antigens was detected by inductively coupled plasma mass spectroscopy (ICP-AES)[16]. …”

Section: Methodsmentioning

confidence: 99%

Colloidal gold nanoparticle probe-based immunochromatographic assay for the rapid detection of chromium ions in water and serum samples

Liu

Xiang

Tang

et al. 2012

Analytica Chimica Acta

135

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An immunochromatographic assay (ICA) using gold nanoparticles coated with monoclonal antibody (McAb) for the detection of chromium ions (Cr) in water and serum samples was developed, optimized and validated. Gold nanoparticles coated with affinity-purified monoclonal antibodies against isothiocyanobenzyl-EDTA (iEDTA)-chelated Cr3+ were used as the detecting reagent in this completive immunoassay-based one-step test strip. The ICA was investigated to measure chromium speciation (Cr3+ and Cr6+ ions) in water samples. Chromium standard samples of 0-80 ng/mL in water were determined by the test strips. The results showed that the visual lowest detection limit (LDL) of the test strip was 50.0 ng/mL. A portable colorimetric lateral flow reader was used for the quantification of Cr. The results indicated that the linear range of the ICA with colorimetric detection was 5-80 ng/mL. The ICA was also validated for the detection of chromium ions in serum samples. The test trips showed high stability in that they could be stored at 37°C for at least 12 weeks without significant loss of activity. The test strip also showed good selectivity for Cr detection with negligible interference from other heavy metals. Because of its low cost and short testing time (within 5 min), the test strip is especially suitable for on-site large-scale screening of Cr-polluted water samples, biomonitoring of Cr exposure, and many other field applications.

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Section: Methodsmentioning

confidence: 99%

Colloidal gold nanoparticle probe-based immunochromatographic assay for the rapid detection of chromium ions in water and serum samples

Liu

Xiang

Tang

et al. 2012

Analytica Chimica Acta

135

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“…The hapten and carrier protein have different characteristic absorption peaks. Therefore, after successful coupling of hapten and carrier protein, the conjugate should have characteristic absorption peaks of both hapten and carrier protein, or due to structural changes, the characteristic absorption peaks of artificial antigen will be shifted relative to the original carrier protein ( Kong et al, 2010 ). As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

A monoclonal antibody-based time-resolved fluorescence microsphere lateral flow immunoassay for paclobutrazol detection

Cheng

Xie

Liang

et al. 2022

Current Research in Food Science

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“…No potential conflict of interest was reported by the authors. (Kong et al, 2010;Quinn et al, 2018;Xing, Feng et al 2013…”

Section: Disclosure Statementmentioning

confidence: 99%

Rapid immunological detection of copper ions using fluorescence immunochromatographic assay

Yang

Long-qiu

Kang³

et al. 2018

Food and Agricultural Immunology

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One-step fluorescence immunochromatographic assay (FICA) has been developed using fluorescent microspheres-labelled monoclonal antibody (McAb) probe for the rapid detection of copper ions in herbal plants and soil samples were investigated in this study. The results can be read by the fluorescence reader according to the fitted standard curve equation. Under optimal conditions, detecting copper ions with FICA can be done within 15 min with a LOD (limit of detection) of 0.2 μg/L, and was very useful for the rapid onsite testing. A total of 31 solid samples were pre-treated by microwave digestion combined with cation exchange solid phase extraction and prepared as test samples for FICA and ICP-AES. The two methods showed good relativity with correlation coefficients of 0.924 and 0.969 in herbal plants and soil samples for copper detection, respectively. We hope that our research can help promote the daily monitoring of heavy metal pollution effectively.

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Synthesis and identification of artificial antigens for cadmium and copper

Cited by 21 publications

References 14 publications

Colloidal gold nanoparticle probe-based immunochromatographic assay for the rapid detection of chromium ions in water and serum samples

Colloidal gold nanoparticle probe-based immunochromatographic assay for the rapid detection of chromium ions in water and serum samples

A monoclonal antibody-based time-resolved fluorescence microsphere lateral flow immunoassay for paclobutrazol detection

Rapid immunological detection of copper ions using fluorescence immunochromatographic assay

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