A monoclonal antibody-based time-resolved fluorescence microsphere lateral flow immunoassay for paclobutrazol detection

Cheng, Yongjian; Xie, Bin; Liang, Yifan; Liu, Xinmei; Chen, Haojie; Li, Jiadong; Lei, Hongtao; Xiao, Zhengguo

doi:10.1016/j.crfs.2022.08.017

Cited by 7 publications

(2 citation statements)

References 38 publications

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“…One week after the third immunization, antisera were collected from the mouse tail for sensitivity testing by icELISA. Four consecutive immunizations were conducted, and mice with the highest potency and optimal inhibition rate were selected for cell fusion [29]. A boost immunization was performed three days before cell fusion by injecting 200 µL of the immunogen directly into the mouse abdominal cavity.…”

Section: Preparation and Characterization Of Pic Monoclonal Antibodymentioning

confidence: 99%

Development of a Time-Resolved Fluorescent Microsphere Test Strip for Rapid, On-Site, and Sensitive Detection of Picoxystrobin in Vegetables

Chen,

Zhang

et al. 2024

Foods

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Picoxystrobin (PIC) is a fungicide extensively used for disease control in both crops and vegetables. Residues of PIC in vegetables pose a potential threat to human health due to their accumulation in the food chain. In this study, a specific PIC monoclonal antibody (mAb) was developed by introducing a carboxylic acid arm into PIC and subsequently preparing a hapten and an artificial antigen. A sensitive and rapid time-resolved fluorescence immunochromatographic assay (TRFICA) was established based on the mAb. Subsequently, using a time-resolved fluorescent microsphere (TRFM) as signal probe, mAbs and microspheres were covalently coupled. The activated pH, the mAb diluents, the mAb amount, and the probe amount were optimized. Under optimized conditions, the quantitative limits of detection (qLOD) of PIC in cucumber, green pepper, and tomato using TRFICA were established at 0.61, 0.26, and 3.44 ng/mL, respectively; the 50% inhibiting concentrations (IC50) were 11.76, 5.29, and 37.68 ng/mL, respectively. The linear ranges were 1.81–76.71, 0.80–35.04, and 8.32–170.55 ng/mL, respectively. The average recovery in cucumber, green pepper, and tomato samples ranged from 79.8% to 105.0%, and the corresponding coefficients of variation (CV) were below 14.2%. In addition, 15 vegetable samples were selected and compared with the results obtained using ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS). The results revealed a high degree of concordance between the proposed method and UPLC-MS/MS. In conclusion, the devised TRFICA method is a valuable tool for rapid, on-site, and highly sensitive detection of PIC residues in vegetables.

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Section: Preparation and Characterization Of Pic Monoclonal Antibodymentioning

confidence: 99%

Development of a Time-Resolved Fluorescent Microsphere Test Strip for Rapid, On-Site, and Sensitive Detection of Picoxystrobin in Vegetables

Chen,

Zhang

et al. 2024

Foods

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“…As a gold standard for the quantitative detection of antigens, enzyme-linked immunosorbent assay (ELISA) is a relatively simple and practical method with good sensitivity. However, the traditional ELISA still needs a long incubation time and complicated washing steps, and it adopts the colorimetric signal readout, which makes it susceptible to interference, impeding its application for rapid and sensitive detection. Therefore, developing a rapid, simple, and highly sensitive analytical approach for Cd 2+ detection is urgent and necessary.…”

Section: Introductionmentioning

confidence: 99%

Time-Resolved FRET-Based Immunosensor for the Ultrasensitive and Rapid Detection of Cd²⁺

Han

Pan

Chen

et al. 2023

J. Agric. Food Chem.

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The detection of heavy metals in food is beneficial to public health. Herein, a new time-resolved fluorescent immunosensor based on the Forster resonance energy transfer (FRET) was proposed to rapidly detect a cadmium ion (Cd 2+ ). After coupling with an antigen and a monoclonal antibody (mAb), respectively, the synthesized europium (III)-chelate-doped polystyrene time-resolved fluorescence microsphere-antigen (Eu-antigen) and gold nanoflowers-mAb (GNFs-mAb) interacted by antigen− antibody combination to quench fluorescence through FRET. Under optimal conditions, the Eu-GNF immunosensing platform provided a quick response to Cd 2+ within 30 min. The limit of detection was 0.29 ng/mL with a linear range of 1−500 ng/mL. This immunosensor was further validated via graphite furnace atomic absorption spectrometry and exhibited satisfactory recovery for the detection of Cd 2+ in four kinds of tea samples (98.82−108.01%). Thus, this strategy provides a good paradigm for the rapid and high-throughput detection of heavy metals in field testing.

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Time-resolved fluorescent microsphere-based lateral flow assay combined with polymerase chain reaction for quantitatively detecting beef to assess the authenticity of beef products

Jing,

Liu,

Tao

2024

Food Control

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A monoclonal antibody-based time-resolved fluorescence microsphere lateral flow immunoassay for paclobutrazol detection

Cited by 7 publications

References 38 publications

Development of a Time-Resolved Fluorescent Microsphere Test Strip for Rapid, On-Site, and Sensitive Detection of Picoxystrobin in Vegetables

Development of a Time-Resolved Fluorescent Microsphere Test Strip for Rapid, On-Site, and Sensitive Detection of Picoxystrobin in Vegetables

Time-Resolved FRET-Based Immunosensor for the Ultrasensitive and Rapid Detection of Cd²⁺

Time-resolved fluorescent microsphere-based lateral flow assay combined with polymerase chain reaction for quantitatively detecting beef to assess the authenticity of beef products

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A monoclonal antibody-based time-resolved fluorescence microsphere lateral flow immunoassay for paclobutrazol detection

Cited by 7 publications

References 38 publications

Development of a Time-Resolved Fluorescent Microsphere Test Strip for Rapid, On-Site, and Sensitive Detection of Picoxystrobin in Vegetables

Development of a Time-Resolved Fluorescent Microsphere Test Strip for Rapid, On-Site, and Sensitive Detection of Picoxystrobin in Vegetables

Time-Resolved FRET-Based Immunosensor for the Ultrasensitive and Rapid Detection of Cd2+

Time-resolved fluorescent microsphere-based lateral flow assay combined with polymerase chain reaction for quantitatively detecting beef to assess the authenticity of beef products

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Product

Resources

About

Time-Resolved FRET-Based Immunosensor for the Ultrasensitive and Rapid Detection of Cd²⁺