Synthesis and evaluation of gold(III) complexes as efficient DNA binders and cytotoxic agents

Patel, Mohan N.; Bhatt, Bhupesh S.; Dosi, Promise A.

doi:10.1016/j.saa.2013.03.037

Cited by 30 publications

(13 citation statements)

References 25 publications

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“…Data are presented as ( η / η 0 ) 1/3 versus [complex]/[DNA], where η is the viscosity of DNA in the presence of complex and η 0 is the viscosity of DNA alone. Viscosity values were calculated from the observed flow time of DNA‐containing solutions (t) corrected for that of the buffer alone (t 0 ), η = (t − t 0 ) …”

Section: Methodsmentioning

confidence: 99%

Fluorescence and absorption studies of DNA–Pd(II) complex interaction: Synthesis, spectroanalytical investigations and biological activities

et al. 2019

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Novel palladium(II) complexes (7a–7e) of substituted quinoline derivatives were synthesized. The complexes were characterized using various techniques such as thermogravimetric analysis (TGA), elemental analysis, conductance measurement, mass, absorption, infra‐red (IR), 1H NMR, 13C NMR and energy‐dispersive X‐ray spectroscopy (EDX). Complexes for herring sperm DNA (HS DNA) binding were explored and absorption titration and the binding constant (Kb) as well as Gibb's free energy were evaluated. Complex 7d exhibited the highest binding constant, therefore the thermodynamic parameters of 7d at different temperatures were evaluated. To support the results of the absorption titration, fluorescence titration, viscosity measurement and molecular docking studies were performed. The fluorescence quenching data as evaluated from Stern–Volmer equation were used to calculate KSV, Kf and the number of binding sites. The results of all these studies were in good agreement with the absorption study. DNA electrophoretic mobility was performed to explore the possible application of metal complexes as artificial metallonucleases. The antibacterial activity of the complexes was accessed against different pathogenic bacteria and cytotoxicity was measured using brine shrimp and S. pombe.

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Section: Methodsmentioning

confidence: 99%

Fluorescence and absorption studies of DNA–Pd(II) complex interaction: Synthesis, spectroanalytical investigations and biological activities

et al. 2019

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“…Although cancer cell death can be achieved effectively, chemotherapy is often non-selective and targets the DNA of normal cells as well to causing undesirable side effects [ 125 , 126 ]. Many efforts are, therefore, directed towards identifying metal complexes with greater selectivity, such as gold(I) and, especially, gold(III) complexes with a d 8 electronic confirmation as found in platinum(II) anti-cancer drugs, although generally, they are reported to have a poorer DNA binding ability [ 11 , 127 , 128 , 129 ]. Further, gold(III) complexes, bearing higher amounts of positive charge than their gold(I) congeners, should possess a greater affinity towards the relatively negatively charged DNA [ 130 , 131 ].…”

Section: Gold Compounds and Their Biological Mechanisms Of Actionmentioning

confidence: 99%

Gold-Based Medicine: A Paradigm Shift in Anti-Cancer Therapy?

2018

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A new era of metal-based drugs started in the 1960s, heralded by the discovery of potent platinum-based complexes, commencing with cisplatin [(H3N)2PtCl2], which are effective anti-cancer chemotherapeutic drugs. While clinical applications of gold-based drugs largely relate to the treatment of rheumatoid arthritis, attention has turned to the investigation of the efficacy of gold(I) and gold(III) compounds for anti-cancer applications. This review article provides an account of the latest research conducted during the last decade or so on the development of gold compounds and their potential activities against several cancers as well as a summary of possible mechanisms of action/biological targets. The promising activities and increasing knowledge of gold-based drug metabolism ensures that continued efforts will be made to develop gold-based anti-cancer agents.

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“…The hydrodynamic volume measurement study was carried out using an Ubbelohed viscometer kept under the thermostatic bath at constant temperature 27 ± 0.1 °C. The experiment was carried out according to literature …”

Section: Methodsmentioning

confidence: 99%

Synthesis, characterization and biological applications of some substituted pyrazoline based palladium (II) compounds

Thakor

Lunagariya

Bhatt

et al. 2018

Applied Organom Chemis

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Palladium (II) complexes of the type [PdLCl 2 ] (where L = substituted pyrazoline ligands) have been synthesized. The metal complexes (5a-5f) have been characterized by various spectroscopic and analytical techniques like 1 H-NMR, 13 C-NMR, LC-MS, IR, Energy-dispersive X-ray (EDX), electronic spectroscopy, thermogravimetric analysis (TGA), elemental analysis and conductance measurement. Complexes to herring sperm DNA (HS-DNA) binding has been explored by absorption titration and binding constant (K b ) as well as Gibb's free energy have been evaluated. Complex 5b shows the highest binding constant value. So, thermodynamic parameters of 5b-HS DNA complex at different temperature have been evaluated. Also, Viscosity measurement and molecular modeling studies have been performed to know the binding mode of complexes. Based on the observations, an intercalative binding mode of DNA has been proposed. Further confirmation of intercalative mode of DNA binding has been taken by fluorescence spectroscopy and results are in a good agreement with absorption titration, viscosity measurement and molecular modeling data. Antibacterial activity of the complexes has been screened against pathogenic bacteria such as S. aureus, E. coli, B. subtilis, S. marcescens and P. aeruginosa. Cytotoxicity is performed on brine shrimp and S. pombe. Gel electrophoresis assay demonstrates that all the complexes can cleave the pUC19 plasmid DNA. Anti-tuberculosis activity has been carried out using mycobacterium tuberculosis H 37 Rv bacteria by L.J. Medium conventional method.

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Synthesis and evaluation of gold(III) complexes as efficient DNA binders and cytotoxic agents

Cited by 30 publications

References 25 publications

Fluorescence and absorption studies of DNA–Pd(II) complex interaction: Synthesis, spectroanalytical investigations and biological activities

Fluorescence and absorption studies of DNA–Pd(II) complex interaction: Synthesis, spectroanalytical investigations and biological activities

Gold-Based Medicine: A Paradigm Shift in Anti-Cancer Therapy?

Synthesis, characterization and biological applications of some substituted pyrazoline based palladium (II) compounds

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