Fluorescence and absorption studies of DNA–Pd(II) complex interaction: Synthesis, spectroanalytical investigations and biological activities

Abstract

Novel palladium(II) complexes (7a–7e) of substituted quinoline derivatives were synthesized. The complexes were characterized using various techniques such as thermogravimetric analysis (TGA), elemental analysis, conductance measurement, mass, absorption, infra‐red (IR), 1H NMR, 13C NMR and energy‐dispersive X‐ray spectroscopy (EDX). Complexes for herring sperm DNA (HS DNA) binding were explored and absorption titration and the binding constant (Kb) as well as Gibb's free energy were evaluated. Complex 7d exhibited the highest binding constant, therefore the thermodynamic parameters of 7d at different temperatures were evaluated. To support the results of the absorption titration, fluorescence titration, viscosity measurement and molecular docking studies were performed. The fluorescence quenching data as evaluated from Stern–Volmer equation were used to calculate KSV, Kf and the number of binding sites. The results of all these studies were in good agreement with the absorption study. DNA electrophoretic mobility was performed to explore the possible application of metal complexes as artificial metallonucleases. The antibacterial activity of the complexes was accessed against different pathogenic bacteria and cytotoxicity was measured using brine shrimp and S. pombe.

Heteroleptic C, N- Donor Pd(II) Complexes: Synthesis, Characterization, DNA/BSA Binding Interactions And Biological Studies

“…Complexes I, III and V exhibit signi cant activity than complexes II, IV and VI against ve organisms. The coordination compounds (45-105 µm) show signi cant activity than ligands (255-375 µM), and are comparable with reported palladium (II) complexes [27]. Graphical representation of MIC value of ligand(L 1 -L 6 ) and Pd(II) complexes(I-VI) is shown in Fig.…”

“…Measurement of viscosity is a simple, complementary and excellent method to know the binding mode of metal complexes to DNA. The detailed experimental process was as reported in the literature [27].…”

Spectroscopic and electrochemical study for evaluating DNA interaction activity of 4‐(3‐halophenyl)‐6‐(pyridin‐2‐yl)pyrimidin‐2‐amine based piano stool Cp* Rh (III) and Ir (III) complexes

“…Visualization of the docked pose has been done using the Discovery Studio 3.5 software molecular graphics program. The default parameters were used for the docking calculations with correlation type shape only, FFT mode at 3D level, grid dimension of 6 with receptor range 180 and ligand range 180 with twist range 360 and distance range 40 …”

Biomolecular Interaction, Antibacterial and Anticancer Activities of Organometallic Re(I) Complexes with 5-(2-butyl-5-chloro-1H-imidazol-4-yl)-1,3-diaryl-4,5-dihydro-1H-pyrazole

“…To determine the theoretical binding energy of synthesized compounds to DNA, docking study was performed using HEX 6.0 software [40]. The .pdb les of complex coordinates were obtained by converting their .mol le using CHIMERA 1.…”