2019
DOI: 10.1016/j.freeradbiomed.2018.11.013
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Synthesis and evaluation of 13C-labeled 5-5-dimethyl-1-pyrroline-N-oxide aimed at in vivo detection of reactive oxygen species using hyperpolarized 13C-MRI

Abstract: Effective means to identify the role of reactive oxygen species (ROS) mediating several diseases including cancer, ischemic heart disease, stroke, Alzheimer's and other inflammatory conditions in in vivo models would be useful. The cyclic nitrone 5,5-Dimethyl-1-pyrroline-N-oxide (DMPO) is a spin trap frequently used to detect free radicals in vitro using Electron Paramagnetic Resonance (EPR) spectroscopy. In this study, we synthesized 13 C-labeled DMPO for hyperpolarization by dynamic nuclear polarization, in … Show more

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Cited by 10 publications
(7 citation statements)
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“…HP 13 C MRI, wherein more than 10,000 times enhancement of the MRI signal of 13 C-labeled redox-sensitive metabolic tracers can be realized, has enabled real-time, noninvasive imaging of metabolism in vivo and opened new research avenues for the field of redox biology. Although more than 60 13 C metabolic tracers have been reported and attempts have been made at developing specific tracers for detecting ROS ( 60 ), to date only a few metabolic tracers have been established for monitoring tissue redox state. As such, future development of new redox-sensitive metabolic tracers and a comprehensive understanding of the correlation between metabolic alterations and the redox state are essential.…”
Section: Applications Of Hp 13 C Mri In Various Diseasesmentioning
confidence: 99%
“…HP 13 C MRI, wherein more than 10,000 times enhancement of the MRI signal of 13 C-labeled redox-sensitive metabolic tracers can be realized, has enabled real-time, noninvasive imaging of metabolism in vivo and opened new research avenues for the field of redox biology. Although more than 60 13 C metabolic tracers have been reported and attempts have been made at developing specific tracers for detecting ROS ( 60 ), to date only a few metabolic tracers have been established for monitoring tissue redox state. As such, future development of new redox-sensitive metabolic tracers and a comprehensive understanding of the correlation between metabolic alterations and the redox state are essential.…”
Section: Applications Of Hp 13 C Mri In Various Diseasesmentioning
confidence: 99%
“…In the spin probe technique, decay in DNP enhancement can thus be used as a quantitative indicator of ROS . Similarly, change in chemical shift of hyperpolarized spin traps upon intermediate adduct-formation with ROS provides another way to detect and quantify ROS. …”
Section: Applications Of Liquid-state Dnp Nmrmentioning
confidence: 99%
“…89 Similarly, change in chemical shift of hyperpolarized spin traps upon intermediate adduct-formation with ROS provides another way to detect and quantify ROS. 90 3.7. Increasing the Sensitivity of Alternative NMR Methodologies and Low-Field NMR.…”
Section: Applications Of Liquid-state Dnp Nmrmentioning
confidence: 99%
“…Unfortunately, dehydroascorbic acid causes transient respiratory arrest at relatively low concentrations (10 mg kg −1 ) in mice 10 as well as pancreatic toxicity 13 , conditions which may limit its translational potential. Hyperpolarized spin trap probes based on DMPO have been developed but are limited to detecting ROS production 14 , 15 . Toxicity concerns have also been expressed for the lanthanide based redox sensitive PARACEST MRI contrast agents 16 , 17 .…”
Section: Introductionmentioning
confidence: 99%