Synthesis and characterization of Zinc Oxide nanoparticles using marine Streptomyces sp. with its investigations on anticancer and antibacterial activity

Balraj, B.; Chidambaram, Siva; Krithikadevi, R.; Julie, A Spicer; Potheher, I. Vetha; Senthilkumar, N.

doi:10.1007/s11164-016-2766-6

Cited by 84 publications

(27 citation statements)

References 25 publications

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“…These absorption peaks provide evidence which indicates that the biosynthesized ZnO NPs have stabilized with proteins secreted in the CB and CFS solutions. Several studies have reported that proteins are responsible for stabilizing ZnO NPs by preventing their agglomeration 35 – 37 . For example, Raliya and Tarafdar 35 have demonstrated the synthesis of ZnO NPs using fungi, which secretes a protein that acts as a capping agent to prevent aggregation, thus, stabilizing NPs.…”

Section: Resultsmentioning

confidence: 99%

“…However, its biocompatibility with biological organisms is a major concern. ZnO NPs have been reported to exert cytotoxic effects on various cell lines 17,31,37 . Nevertheless, the cytotoxic effects are highly dependent on many parameters, including the characteristics of NPs (shape and size), synthesis methods, and the type of cell line used 2,54 .…”

Section: Ftir Analysis the Bioactive Molecules Composition Of Cfs Anmentioning

confidence: 99%

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Biosynthesis of zinc oxide nanoparticles by cell-biomass and supernatant of Lactobacillus plantarum TA4 and its antibacterial and biocompatibility properties

Rahman

Mohamad

Zaidan

et al. 2020

Sci Rep

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This study aims to utilize the cell-biomass (CB) and supernatant (CFS) of zinc-tolerant Lactobacillus plantarum TA4 as a prospective nanofactory to synthesize ZnO NPs. The surface plasmon resonance for the biosynthesized ZnO NPs-CFS and ZnO NPs-CB was 349 nm and 351 nm, respectively, thereby confirming the formation of ZnO NPs. The FTIR analysis revealed the presence of proteins, carboxyl, and hydroxyl groups on the surfaces of both the biosynthesized ZnO NPs that act as reducing and stabilizing agents. The DLS analysis revealed that the poly-dispersity indexes was less than 0.4 for both ZnO NPs. In addition, the HR-TEM micrographs of the biosynthesized ZnO NPs revealed a flower-like pattern for ZnO NPs-CFS and an irregular shape for ZnO NPs-CB with particles size of 291.1 and 191.8 nm, respectively. In this study, the biosynthesized ZnO NPs exhibited antibacterial activity against pathogenic bacteria in a concentration-dependent manner and showed biocompatibility with the Vero cell line at specific concentrations. Overall, CFS and CB of L. plantarum TA4 can potentially be used as a nanofactory for the biological synthesis of ZnO NPs.

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Section: Resultsmentioning

confidence: 99%

Section: Ftir Analysis the Bioactive Molecules Composition Of Cfs Anmentioning

confidence: 99%

Biosynthesis of zinc oxide nanoparticles by cell-biomass and supernatant of Lactobacillus plantarum TA4 and its antibacterial and biocompatibility properties

Rahman

Mohamad

Zaidan

et al. 2020

Sci Rep

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“…Moreover, the synthesized ZnONPs exhibited potent antimicrobial activity compared to the standard antimicrobials (amoxicillin/clavulanic acid and nystatin). Previous reports have demonstrated the antimicrobial activity of ZnONPs against several bacterial and fungal species; P. aeruginosa (Jayaseelan et al ; Rajabairavi et al ), Enterococcus sp., S. aureus and P. mirabilis (Chauhan et al ), E.coli and B. subtilis (Balraj et al ), S. aureus and E. coli (Khatami et al ), B. subtilis , E. coli and P. fluorescens (Jiang et al ), H. pylori (Saravanan et al ), A. flavus (Jayaseelan et al ), A. alternata , A. niger , B. cinerea , F. oxysporum and P. expansum (Jamdagni et al ), B. subtilis , S. aureus , E. faecalis , E. coli , P. aeruginosa , K. pneumoniae , C. albicans and C. tropicalis (Abd Elkodous et al ; Gao et al ). Our results further showed that the estimated MIC value of the synthesized ZnONPs against the tested microbes was in the range of 50–100 µg ml −1 .…”

Section: Discussionmentioning

confidence: 99%

Biosynthesis of zinc oxide nanoparticles with antimicrobial, anticancer, antioxidant and photocatalytic activities by the endophytic Alternaria tenuissima

2020

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Aims Zinc oxide nanoparticles (ZnONPs) were successfully synthesized using the culture filtrate of the endophytic fungus Alternaria tenuissima as a rapid, eco‐friendly and cost‐effective method. Methods and Results The rapid synthesis of ZnONPs was completed after 20 min as confirmed by UV–Vis spectroscopy. The synthesized ZnONPs showed a single‐phase crystalline structure. Dynamic light scattering analysis showed that the synthesized ZnONPs were monodispersed and the recorded polydispersity index value was 0·311. Zeta potential value of –23·92 mV indicated the high stability of ZnONPs. Transmission electron microscope revealed the spherical shape and the mean particle size was 15.45 nm. Functional groups present in the prepared samples of ZnONPs were confirmed by Fourier transform infrared spectroscopy. Additionally, the biological activities of in vitro antimicrobial, anticancer, antioxidant as well as the photocatalytic activities were evaluated. ZnONPs showed broad spectrum of antimicrobial potential against all the tested plant and human pathogens. Based on the MTT assay, ZnONPs inhibited the proliferation of normal human melanocytes, human breast and liver cancer cell lines with IC50 concentrations of 55·76, 18·02 and 16·87 µg ml−1. ZnONPs exhibited promising antioxidant potential with 50% inhibitory concentration of 102·13 µg ml−1. Moreover, ZnONPs showed efficient degradation of methylene blue dye. Conclusions The synthesized ZnONPs showed promising activities that can be better explored in the near future for many medical, agricultural and industrial applications. Significance and Impact of the Study This study suggests a new and alternate approach with the excellent biotechnological potentiality for the production of ZnONPs which could open up the way for the industrial manufacture of nanoparticles using microbial platforms.

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“…Studies have shown that protein produced and secreted by microbes play an important role in the NPs synthesis [57, 89–91]. Nevertheless, some studies suggested that the native form of the protein is not compulsory for the NPs synthesis process.…”

Section: Introductionmentioning

confidence: 99%

Microbial synthesis of zinc oxide nanoparticles and their potential application as an antimicrobial agent and a feed supplement in animal industry: a review

Rahman

Mohamad

Zaidan

2019

J Animal Sci Biotechnol

349

134

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In recent years, zinc oxide nanoparticles (ZnO NPs) have gained tremendous attention attributed to their unique properties. Notably, evidence has shown that zinc is an important nutrient in living organisms. As such, both prokaryotes and eukaryotes including bacteria, fungi and yeast are exploited for the synthesis of ZnO NPs by using microbial cells or enzyme, protein and other biomolecules compounds in either an intracellular or extracellular route. ZnO NPs exhibit antimicrobial properties, however, the properties of nanoparticles (NPs) are depended upon on their size and shape, which make them specific for various applications. Nevertheless, the desired size and shape of NPs can be obtained through the optimization process of microbes mediated synthesis by manipulating their reaction conditions. It should be noted that ZnO NPs are synthesized by various chemical and physical methods. Nonetheless, these methods are expensive and not environmentally friendly. On that account, the microbes mediated synthesis of ZnO NPs have rapidly evolved recently where the microbes are cleaner, eco-friendly, non-toxic and biocompatible as the alternatives to chemical and physical practices. Moreover, zinc in the form of NPs is more effective than their bulk counterparts and thus, they have been explored for many potential applications including in animals industry. Notably, with the advent of multi-drug resistant strains, ZnO NPs have emerged as the potential antimicrobial agents. This is mainly due to their superior properties in combating a broad spectrum of pathogens. Moreover, zinc is known as an essential trace element for most of the biological function in the animal’s body. As such, the applications of ZnO NPs have been reported to significantly enhance the health and production of the farm animals. Thus, this paper reviews the biological synthesis of ZnO NPs by the microbes, the mechanisms of the biological synthesis, parameters for the optimization process and their potential application as an antimicrobial agent and feed supplement in the animal industry as well as their toxicological hazards on animals.

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Synthesis and characterization of Zinc Oxide nanoparticles using marine Streptomyces sp. with its investigations on anticancer and antibacterial activity

Cited by 84 publications

References 25 publications

Biosynthesis of zinc oxide nanoparticles by cell-biomass and supernatant of Lactobacillus plantarum TA4 and its antibacterial and biocompatibility properties

Biosynthesis of zinc oxide nanoparticles by cell-biomass and supernatant of Lactobacillus plantarum TA4 and its antibacterial and biocompatibility properties

Biosynthesis of zinc oxide nanoparticles with antimicrobial, anticancer, antioxidant and photocatalytic activities by the endophytic Alternaria tenuissima

Microbial synthesis of zinc oxide nanoparticles and their potential application as an antimicrobial agent and a feed supplement in animal industry: a review

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