Biosynthesis of zinc oxide nanoparticles by cell-biomass and supernatant of Lactobacillus plantarum TA4 and its antibacterial and biocompatibility properties

Rahman, Nor'aini Abdul; Mohamad, Rosfarizan; Zaidan, Uswatun Hasanah; Samsudin, Anjas Asmara

doi:10.1038/s41598-020-76402-w

Cited by 114 publications

(60 citation statements)

References 63 publications

(104 reference statements)

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“…Agar well diffusion method was used to investigate the antibacterial activity of biosynthesized ZnO NPs against poultry-associated foodborne pathogens following the procedure outlined in our previous study [26]. Briefly, the bacterial strains were grown until they reached the 0.5 McFarland turbidity standards and a lawn of bacterial strain was made by spreading them uniformly onto a nutrient agar (NA) (Merck, Darmstadt, Germany) plate using a sterile cotton swab.…”

Section: In Vitro Antibacterial Activity Of Biosynthesized Zinc Oxide Nanoparticles 241 Agar Well Diffusion Methodsmentioning

confidence: 99%

“…The established procedure from our previous study [26] was followed for the biosynthesis of ZnO NPs with some modifications. Briefly, 20 mL of supernatant from zinc-tolerant L. plantarum TA4 was mixed with 80 mL of 500 mM aqueous zinc nitrate (Zn(NO 3 ) 2 •6H 2 O) solution.…”

Section: Zinc Oxide Nanoparticles Preparation and Characterizationmentioning

confidence: 99%

“…The present work involves the biological synthesis of ZnO NPs using Lactobacillus plantarum TA4 from our previous study [26]. The biosynthesized ZnO NPs were analyzed by UV-Visible spectroscopy, and the size and shape morphology was determined using a high-resolution transmission electron microscope (HR-TEM).…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial Potential of Biosynthesized Zinc Oxide Nanoparticles against Poultry-Associated Foodborne Pathogens: An In Vitro Study

Rahman

Mohamad

Zaidan

et al. 2021

Animals

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Since the emergence of multidrug-resistant bacteria in the poultry industry is currently a serious threat, there is an urgent need to develop a more efficient and alternative antibacterial substance. Zinc oxide nanoparticles (ZnO NPs) have exhibited antibacterial efficacy against a wide range of microorganisms. Although the in vitro antibacterial activity of ZnO NPs has been studied, little is known about the antibacterial mechanisms of ZnO NPs against poultry-associated foodborne pathogens. In the present study, ZnO NPs were successfully synthesized using Lactobacillus plantarum TA4, characterized, and their antibacterial potential against common avian pathogens (Salmonella spp., Escherichia coli, and Staphylococcus aureus) was investigated. Confirmation of ZnO NPs by UV-Visual spectroscopy showed an absorption band center at 360 nm. Morphologically, the synthesized ZnO NPs were oval with an average particle size of 29.7 nm. Based on the dissolution study of Zn2+, ZnO NPs released more ions than their bulk counterparts. Results from the agar well diffusion assay indicated that ZnO NPs effectively inhibited the growth of the three poultry-associated foodborne pathogens. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were assessed using various concentrations of ZnO NPs, which resulted in excellent antibacterial activity as compared to their bulkier counterparts. S. aureus was more susceptible to ZnO NPs compared to the other tested bacteria. Furthermore, the ZnO NPs demonstrated substantial biofilm inhibition and eradication. The formation of reactive oxygen species (ROS) and cellular material leakage was quantified to determine the underlying antibacterial mechanisms, whereas a scanning electron microscope (SEM) was used to examine the morphological changes of tested bacteria treated with ZnO NPs. The findings suggested that ROS-induced oxidative stress caused membrane damage and bacterial cell death. Overall, the results demonstrated that ZnO NPs could be developed as an alternative antibiotic in poultry production and revealed new possibilities in combating pathogenic microorganisms.

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Section: In Vitro Antibacterial Activity Of Biosynthesized Zinc Oxide Nanoparticles 241 Agar Well Diffusion Methodsmentioning

confidence: 99%

Section: Zinc Oxide Nanoparticles Preparation and Characterizationmentioning

confidence: 99%

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Antibacterial Potential of Biosynthesized Zinc Oxide Nanoparticles against Poultry-Associated Foodborne Pathogens: An In Vitro Study

Rahman

Mohamad

Zaidan

et al. 2021

Animals

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“…The antimicrobial activity of biosynthesized ZnONPs was studied against Gram-positive ( S. epidermidis and S. aureus ) and Gram-negative ( Salmonella sp. and E. coli ), and it was observed that ZnONPs were more effective against Gram-positive bacteria [ 123 ]. Another amnestic work reports the biofabrication of ZnO, MnO 2 and MgONPs by deploying a rhizophytic bacteria Paenibacillus polymyxa strain Sx3 [ 124 ].…”

Section: Strategies For Synthesis Of Nanoparticles Using Microbesmentioning

confidence: 99%

Microbe-Mediated Biosynthesis of Nanoparticles: Applications and Future Prospects

et al. 2021

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Nanotechnology is the science of nano-sized particles/structures (~100 nm) having a high surface-to-volume ratio that can modulate the physical, chemical and biological properties of the chemical compositions. In last few decades, nanoscience has attracted the attention of the scientific community worldwide due to its potential uses in the pharmacy, medical diagnostics and disease treatment, energy, electronics, agriculture, chemical and space industries. The properties of nanoparticles (NPs) are size and shape dependent. These characteristic features of nanoparticles can be explored for various other applications such as computer transistors, chemical sensors, electrometers, memory schemes, reusable catalysts, biosensing, antimicrobial activity, nanocomposites, medical imaging, tumor detection and drug delivery. Therefore, synthesizing nanoparticles of desired size, structure, monodispersity and morphology is crucial for the aforementioned applications. Recent advancements in nanotechnology aim at the synthesis of nanoparticles/materials using reliable, innoxious and novel ecofriendly techniques. In contrast to the traditional methods, the biosynthesis of nanoparticles of a desired nature and structure using the microbial machinery is not only quicker and safer but more environmentally friendly. Various microbes, including bacteria, actinobacteria, fungi, yeast, microalgae and viruses, have recently been explored for the synthesis of metal, metal oxide and other important NPs through intracellular and extracellular processes. Some bacteria and microalgae possess specific potential to fabricate distinctive nanomaterials such as exopolysaccharides, nanocellulose, nanoplates and nanowires. Moreover, their ability to synthesize nanoparticles can be enhanced using genetic engineering approaches. Thus, the use of microorganisms for synthesis of nanoparticles is unique and has a promising future. The present review provides explicit information on different strategies for the synthesis of nanoparticles using microbial cells; their applications in bioremediation, agriculture, medicine and diagnostics; and their future prospects.

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“…Namely, evidence-supported, accessible resources which they can use to support workers with concussion in their recovery and return-to-work. Inappropriate communication which is limited to a brief exchange within a doctor's office, an emergency department, or a phone call with a workers' compensation board case manager, may result in poor understanding and difficulty to recall the discharge care or return-to-work plan (39).…”

Section: Comparison With Previous Workmentioning

confidence: 99%

Stakeholder Recommendations to Increase the Accessibility of Online Health Information for Adults Experiencing Concussion Symptoms

Beaton

Hadly

Babul

2021

Front. Public Health

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Background: Concussion is a global public health problem. In Canada, concussion is among the top five reasons for workplace time-loss. Concussion results in physical, cognitive, and/or emotional symptoms that temporarily worsen with physical and mental exertion, such as viewing electronic screens. The Internet is the primary source of consumer health information. Studies on the end-user needs of adults with brain injuries in regards to digital health technologies largely focus on informational content. There is little to no research on the accessibility of screen-based informational websites and smartphone applications among this population.Objective: The aim of this research was to involve stakeholders in the design of a comprehensive educational resource to guide concussion recognition, recovery, and return-to-work, called the Concussion Awareness Training Tool for Workers and Workplaces (CATT WW). In order to ensure both relevant content and appropriate delivery of the information to the target groups, participants were asked whether adaptations could increase the accessibility of online health information for the general adult population experiencing concussion symptoms.Methods: Data have been generated through semi-structured in-depth interviews and focus groups with participants from across British Columbia (BC): workers from various industries who were in the concussion recovery process or had returned to work (n = 31); and healthcare or workplace professionals who support concussion diagnosis, recovery, and return-to-work (n = 16). Data were analyzed using NVivo 12. Before commencing data collection, ethical permission was granted by the University of British Columbia Research Ethics Board (H18-00604), and approval was received from WorkSafeBC Research Services.Results: Participants (n = 47) recommended twenty adaptations or supplements to electronic screen-based digital health technologies.Conclusion: Given the high prevalence of concussion among the working adult population, the symptom exacerbation commonly caused by prolonged use of electronic screens, and the demand for online educational resources, these findings can guide clinicians, researchers, technology developers, employers, and occupational health and safety committees to further support adults in concussion recovery and return-to-work.

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Biosynthesis of zinc oxide nanoparticles by cell-biomass and supernatant of Lactobacillus plantarum TA4 and its antibacterial and biocompatibility properties

Cited by 114 publications

References 63 publications

Antibacterial Potential of Biosynthesized Zinc Oxide Nanoparticles against Poultry-Associated Foodborne Pathogens: An In Vitro Study

Antibacterial Potential of Biosynthesized Zinc Oxide Nanoparticles against Poultry-Associated Foodborne Pathogens: An In Vitro Study

Microbe-Mediated Biosynthesis of Nanoparticles: Applications and Future Prospects

Stakeholder Recommendations to Increase the Accessibility of Online Health Information for Adults Experiencing Concussion Symptoms

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