Synthesis and characterization of thermo- and pH- sensitive hydrogels based on Chitosan-grafted N-isopropylacrylamide via γ-radiation

Cai, Hong; Zhang, Zheng Pu; Sun, Ping; He, Bing Lin; Zhu, X. X.

doi:10.1016/j.radphyschem.2004.10.007

Cited by 76 publications

(22 citation statements)

References 28 publications

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“…Numerous investigations have examined the question of pH sensitivity of PNIPAM hydrogels [47][48][49][50][51][52][53], but these works refer mainly to copolymer systems. Of particular interest is the study of Hirotsu et al [14], which showed that at neutral pH, NIPAM-acrylic acid copolymer gels undergo a discontinuous transition as a function of temperature above a certain acrylic acid content.…”

Section: Acid and Base Solutionsmentioning

confidence: 99%

Search for the Origin of Discrepancies in Osmotic Measurements of the PNIPAM - Water System

Manek

Tombácz

Geissler

et al. 2017

Period. Polytech. Chem. Eng.

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Section: Acid and Base Solutionsmentioning

confidence: 99%

Search for the Origin of Discrepancies in Osmotic Measurements of the PNIPAM - Water System

Manek

Tombácz

Geissler

et al. 2017

Period. Polytech. Chem. Eng.

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“…Successful preparation of these supports ascribes to the signaling domains in the molecules, which can specifically bind to integrin on cell membranes [33]. Various methods consisting of covalent bonding, layer-by-layer self-assembly, plasma treatment, photo-initiated grafting, and γ-irradiationinduced grafting have been utilized to immobilize the biomolecules onto the solid matrix [34,35]. These strategies have several limitations, including low grafting density because of steric hindrance, uncontrollable grafting yield of polymer brushes, and undesired reactions between reactive groups of the brushes and the surface [36].…”

Section: Immobilization Of Huvecsmentioning

confidence: 99%

Screening bioactive compounds from Ligusticum chuanxiong by high density immobilized human umbilical vein endothelial cells

Wang

Liu

et al. 2015

Anal Bioanal Chem

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High throughput screening methodologies play a very important role in screening bioactive compounds from complex media. In this work, a new strategy for attaching cells onto amino microspheres using human umbilical vein endothelial cells (HUVECs) as a probe was developed. The immobilization depended on the specific affinity between integrin on the cells and the RGD peptide, which was coated on poly[oligo (ethylene glycol) methacrylate] by atom transfer radical polymerization. Validated application of the stationary phase was performed in the analysis of Ligusticum chuanxiong extraction by high performance affinity chromatography-mass spectrometry. Three compounds were screened as the bioactive compounds of Ligusticum chuanxiong. Two of them were identified as 3-butyl-hexahydroisobenzofuran-1(3H)-one and tetramethylpyrazine (TMP), whereas the other one remains indistinct. The association constant of vascular endothelial growth factor (VEGF) and TMP binding to VEGF receptor (VEGFR) on HUVECs were calculated to be (1.04 ± 0.08) × 10(11) M(-1) and (9.84 ± 1.11) × 10(8) M(-1) by zonal elution. Molecular docking showed that one hydrogen bond was formed between N atom of TMP and 3-N atom of imidazole group in histidine(223) of VEGFR. Both zonal elution and molecular docking indicated that TMP and VEGF bind to the same site of VEGFR on HUVECs. It is possible to become a promising tool for high throughput screening of the bioactive compounds binding to HUVECs through broad application of the stationary phase.

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“…Cai et al 30 have prepared a graft copolymer of N-isopropylacrylamide onto chitosan using 60 Co g-radiation. Grafting percentage and grafting efficiency were improved by increasing the monomer concentration and radiation dose.…”

Section: Modification Through Graftingmentioning

confidence: 99%

Modified chitosan hydrogels as drug delivery and tissue engineering systems: present status and applications

Giri¹,

Thakur²,

Alexander³

et al. 2012

Acta Pharmaceutica Sinica B

297

137

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Chitosan, a natural cationic polysaccharide, is prepared industrially by the hydrolysis of the aminoacetyl groups of chitin, a naturally available marine polymer. Chitosan is a non-toxic, biocompatible and biodegradable polymer and has attracted considerable interest in a wide range of biomedical and pharmaceutical applications including drug delivery, cosmetics, and tissue engineering. The primary hydroxyl and amine groups located on the backbone of chitosan are responsible for the reactivity of the polymer and also act as sites for chemical modification. However, chitosan has certain limitations for use in controlled drug delivery and tissue engineering. These limitations can be overcome by chemical modification. Thus, modified chitosan hydrogels have gained importance in current research on drug delivery and tissue engineering systems. This paper reviews the general properties of chitosan, various methods of modification, and applications of modified chitosan hydrogels.

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Synthesis and characterization of thermo- and pH- sensitive hydrogels based on Chitosan-grafted N-isopropylacrylamide via γ-radiation

Cited by 76 publications

References 28 publications

Search for the Origin of Discrepancies in Osmotic Measurements of the PNIPAM - Water System

Search for the Origin of Discrepancies in Osmotic Measurements of the PNIPAM - Water System

Screening bioactive compounds from Ligusticum chuanxiong by high density immobilized human umbilical vein endothelial cells

Modified chitosan hydrogels as drug delivery and tissue engineering systems: present status and applications

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