Synthesis and characterization of nontoxic silver nano-particles with preferential bactericidal activity

Abstract

The massive prophylactically or remedially application of antibiotics without proper medical indications lead to severe problems of bacterial resistance. Nanoparticles (NPs) are increasingly applied to target bacterial infections as an antibiotic alternative. Green synthesis of silver nanoparticles (AgNPs), with controlled morphology, within cinnamon extract using microwave irradiation was carried out. The influence of the pH on the synthesized AgNPs was assessed. Physicochemical characterizations were followed through UV, FTIR, FESEM, HRTEM, XPS and XRD analyses. Bactericidal activity and in-vitro cytotoxicity of the achieved AgNPs were investigated. Results proved the successful synthesis of spherical AgNPs with an average size of 15 nm. The AgNPs safety was verified through cytotoxicity test against skin fibroblast normal cells. The obtained AgNPs exhibited promising bactericidal activities against the screening of several Gram+ve and Gram-ve bacteria. Therefore, it can be successfully applied in the biomedical fields for hard and soft tissue remedies.

Collagen-Carboxymethylcellulose Biocomposite Wound-Dressings with Antimicrobial Activity

“…Similar to the qualitative antimicrobial test, no statistically significant differences between the samples containing similar amounts of silver nanoparticles were found. The obtained results are in accordance with other studies reporting the great antimicrobial efficiency of AgNPs, when they are integrated in various polymeric matrices [53,54] and coatings, including wound-dressings [24,55]. The antibactericidal properties of silver are well known; briefly, their mechanism relays on the generation of reactive ions which are able to interfere with various cellular biomolecules, such as lipids, proteins, and even nucleic acids.…”

“…Nanostructured silver particles exhibit unique physical and chemical properties that are even more suitable for biomedical applications than those of silver in any other formula. These properties make silver nanoparticles (AgNP) efficient materials in controlling the progression of various severe infections (e.g., hospital acquired infections, wound infections) [23,24].…”

Recent Advances in Surface Nanoengineering for Biofilm Prevention and Control. Part II: Active, Combined Active and Passive, and Smart Bacteria-Responsive Antibiofilm Nanocoatings

“…Antimicrobial properties of silver have been well-known for a long time, but the mechanism underlying silver nanoparticles-mediated toxicity is still incompletely understood, and the full details of how silver nanoparticles induce toxicity in bacterial cells remain to be elucidated. However, a series of studies have provided important mechanistic insights into the antibacterial properties of both zero-valent silver nanoparticles and silver ions [82][83][84][85].…”

An Updated Review on Silver Nanoparticles in Biomedicine

“…AgNPs with tunable physicochemical characteristics and versatile functionality can be obtained by various top-down (mainly, evaporation-condensation processes of bulk silver) [28,29] and bottom-up (mainly, electrochemical processes of metallic salts) processing methods [30,31]. Special attention was oriented on the inexpensive and environmentally-friendly synthesis of AgNPs, which either considers the revaluation of plant-derived reducing and antioxidant phytochemicals [32,33] or the microorganism-mediated bioreduction mechanisms [34,35].…”