Collagen-Carboxymethylcellulose Biocomposite Wound-Dressings with Antimicrobial Activity

Abstract

Microbial infections associated with skin diseases are frequently investigated since they impact on the progress of pathology and healing. The present work proposes the development of freeze-dried, glutaraldehyde cross-linked, and non-cross-linked biocomposite dressings with a porous structure, which may assist the reepithelization process through the presence of collagen and carboxymethylcellulose, along with a therapeutic antimicrobial effect, due to silver nanoparticles (AgNPs) addition. Phisyco-chemical characterization revealed the porous morphology of the obtained freeze-dried composites, the presence of high crystalline silver nanoparticles with truncated triangular and polyhedral morphologies, as well as the characteristic absorption bands of collagen, silver, and carboxymethylcellulose. In vitro tests also assessed the stability, functionality, and the degradability rate of the obtained wound-dressings. Antimicrobial assay performed on Gram-negative (Escherichia coli), Gram-positive (Staphyloccocus aureus) bacteria, and yeast (Candida albicans) models demonstrated that composite wound dressings based on collagen, carboxymethylcellulose, and AgNPs are suitable for skin lesions because they prevent the risk of infection and have prospective wound healing capacity. Moreover, the cell toxicity studies proved that the obtained materials can be used in long time treatments, with no cytotoxic effects.

Biodistribution of essential oil-conjugated silver nanoparticles

“…Diffraction planes of fcc Ag were evidenced from SAED patterns, which was in compliance with XRD results and previous studies [77,78]. Moreover, as the crystallite size estimated from XRD data was comparable with the particle size estimated from TEM results for all samples, we concluded that spherical monocrystalline Ag@EO NPs were successfully obtained in our study.…”

Recent Developments of Carboxymethyl Cellulose

“…In this regard, numerous structures of such materials have been used. Amongst them, CMC-based hydrogels [250][251][252], films [253,254], fibers [255][256][257], wafers [258][259][260], gauzes [261,262], and nanoparticles [263][264][265] have gained much interest during the past decades.…”

Innovative Eco-Friendly Hydrogel Film for Berberine Delivery in Skin Applications

“…In this work, an innovative, eco-friendly hydrogel, based on a mixture of polysaccharides and clays, has been developed to locally release berberine. Thanks to its biocompatibility, hydrophilicity, biodegradability, tissue resembling, pro-haemostatic properties and low cost [12][13][14][15], the sodium salt carboxymethyl cellulose (CMCNa) was used for the development of hydrogel films using gallium (Ga 3+ ) ions as crosslinkers. This approach represents a novelty in respect to the usual-often toxic-crosslinkers, such as covalent crosslinkers (i.e., carbodiimide crosslinkers, various glycidyl ethers, etc.…”