The bio-nanocomposite role in wastewater treatment is a primary concern of this research. The physical, chemical, mechanical stability and antimicrobial activity of these bio-nanocomposites were investigated. The method is based on the biological reduction of aqueous copper sulphate pentahydrate, lead nitrate, silver nitrate, zinc sulphate heptahydrate salt using seed extract of Eucalyptus globulus as reducing agent at ambient temperature. The synthesised metal nanoparticles (MNPs) were analysed by UV-visible spectroscopy and Fourier transform infrared spectroscopy analyses. An ex-situ method involves constructing bio-nanocomposite by blending MNPs with tea waste activated carbon. Cross-linking in activated carbon takes place which was confirmed by changes in the mixture of components. The present yield of activated carbon was characterised by scanning electron microscopy and energy dispersive X-ray measurements. A few micro or nano range, spherical shape of activated carbon was studied by SEM. The main elements found in the activated carbon by EDX are C, O, S, Ag, Cl and Cu. The efficacy of such active bio-nanocomposite (ABN) tested against human pathogen includes both type of bacteria and fungus. The inhibitory effects of ABN are discernible from the results that reveal biologically inseminated MNPs can be used to clean up the contaminated environment.