Synthesis and Characterization of Copolymeric and Terpolymeric Hydrogel-Silver Nanocomposites Based on Acrylic Acid, Acrylamide and Itaconic Acid: Investigation of Their Antibacterial Activity Against Gram-Negative Bacteria
Abstract:-In this study, copolymeric and terpolymeric hydrogel-silver nanocomposites based on poly(acrylamideco-itaconic acid), poly(acrylic acid-co-itaconic acid) and poly(acrylic acid-co-acrylamide-co-itaconic acid) were synthesized by free-radical polymerization. These nanocomposites were characterized by Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), UV-Visible Spectrophotometry (UVVis) and X-Ray Diffraction (XRD) analysis, as well as their swelling behaviors. In addition, antib… Show more
“…Among those are biocompatible and often biodegradable polymers such as alginate, chitosan (CHI), poly(vinyl alcohol) (PVA), poly(vinyl pyrrolidone) (PVP), cellulose, etc. [3][4][5][6][7][8][9][10][11]. Of course, apart from biocompatibility, hydrogel-based wound dressings must satisfy several other stringent requirements, e.g.…”
<p class="PaperAbstract">Polymer-based hydrogel materials are excellent candidates for new-generation wound dressings with improved properties, such as high sorption ability, good mechanical properties and low adhesiveness. Cross-linked hydrogel matrices also serve as excellent carriers for controlled release of antibacterial agents, such as silver nanoparticles (AgNPs), which are preferred over conventional antibiotics due to low propensity to induce bacterial resistance. In this work, we aim to produce novel silver/poly(vinyl alcohol)/chitosan (Ag/PVA/CHI) hydrogels for wound dressing applications. The electrochemical AgNPs synthesis provided facile and green method for the reduction of Ag<sup>+</sup> ions inside the hydrogel matrices, without the need to use toxic chemical reducing agents. The formation of AgNPs was confirmed using UV-visible spectroscopy, scanning and transmission electron microscopy. Release kinetics was investigated in modified phosphate buffer solution at 37 °C to mimic physiological conditions. Release profiles indicated “burst release” behavior, which is beneficial for wound dressing applications. The antibacterial activity was evaluated against Staphylococcus aureus and Escherichia coli strains using disc-diffusion test, and non-toxicity of hydrogels was proved by dye-exclusion test. The obtained results confirmed strong potential of Ag/PVA/CHI hydrogels for biomedical applications.</p>
“…Among those are biocompatible and often biodegradable polymers such as alginate, chitosan (CHI), poly(vinyl alcohol) (PVA), poly(vinyl pyrrolidone) (PVP), cellulose, etc. [3][4][5][6][7][8][9][10][11]. Of course, apart from biocompatibility, hydrogel-based wound dressings must satisfy several other stringent requirements, e.g.…”
<p class="PaperAbstract">Polymer-based hydrogel materials are excellent candidates for new-generation wound dressings with improved properties, such as high sorption ability, good mechanical properties and low adhesiveness. Cross-linked hydrogel matrices also serve as excellent carriers for controlled release of antibacterial agents, such as silver nanoparticles (AgNPs), which are preferred over conventional antibiotics due to low propensity to induce bacterial resistance. In this work, we aim to produce novel silver/poly(vinyl alcohol)/chitosan (Ag/PVA/CHI) hydrogels for wound dressing applications. The electrochemical AgNPs synthesis provided facile and green method for the reduction of Ag<sup>+</sup> ions inside the hydrogel matrices, without the need to use toxic chemical reducing agents. The formation of AgNPs was confirmed using UV-visible spectroscopy, scanning and transmission electron microscopy. Release kinetics was investigated in modified phosphate buffer solution at 37 °C to mimic physiological conditions. Release profiles indicated “burst release” behavior, which is beneficial for wound dressing applications. The antibacterial activity was evaluated against Staphylococcus aureus and Escherichia coli strains using disc-diffusion test, and non-toxicity of hydrogels was proved by dye-exclusion test. The obtained results confirmed strong potential of Ag/PVA/CHI hydrogels for biomedical applications.</p>
“…Thomas et al [11] have been reported nano particles such as silver, gold, and copper is highly toxic to microorganisms due to their strong biocidal effects. Bal1 et al [12] have prepared itaconic acid based silver nanocomposite hydrogels with a lesser swelling ratio due to Ag + complexation with carboxyl moieties. Tibbitt et al [13], reported that for stem cell engineering, immunomodulation and cancer research applications demand multiple functionalities of the hydrogel network and dynamic interactions between the surrounding cells.…”
Traditional hydrogels have shown substandard multifunctional, mechanical properties in biomedical field. Gold nanoparticles can provided an expanded array of nanostructured materials with exclusive biomedical properties. The nanocomposite hydrogels have expected to exhibit both nanomaterial and hydrogel properties, which may resulted in potential applications in biomedical field. Hence, the present investigation reported simple and greener approach to the preparation and properties of pH-responsive nanocomposite hydrogels (GIAE) based on itaconic acid (IA), acrylic acid, ethylene glycol and colloidal gold nanoparticles. The obtained nanocomposite hydrogels found to have desired exfoliated surface morphology and enhanced pH-sensitive swelling and thermal stability. It also showed appreciable anti-microbial properties due to presence of gold nanoparticles and IA. The cytotoxicity nature of itaconic acid based gold nanocomposite hydrogels were also developed ~ 90% due gold nanoparticles. Moreover, the degradation property of prepared nanocomposite hydrogels have been reduced due to bacterial inhibition tendency of gold nanoparticles. This work provides viewpoints to develop pH-tunable GIAE nanocomposite hydrogels with significant biological properties. The resultant nanocomposite hydrogels may be recommended for biomedical applications mere in future such as pH-sensitive drug delivery, scaffold for tissue engineering, wound healing application, antimicrobial material and nanomedicine etc.
“…The introduction of nanoparticles has allowed the scientific community to enhance the antibacterial properties of silver. The increased surface area of the nanoparticles in turn induces an increased rate of interaction between the test subjects and the ionic silver (Alexander, 2009;Nam et al, 2015;Bal et al, 2015;Dong et al, 2016;Mckevica et al, 2016;Amadio et al, 2017).…”
This work aimed to produce freeze-cast pellets of porous ceramic filters of alumina and kaolinite impregnated with silver nanoparticles and to test the efficiency of bactericidal effects. Silver nanoparticles were characterized by average particle size, zeta potential and TEM. After impregnation, leached water was analyzed by graphite furnace atomic absorption spectrometry (GFAAS) that showed an efficiency of more than 99.9% in silver impregnation. From SEM/EDS, it was possible to observe that the pellets have a homogeneous distribution of silver nanoparticles. A halo of inhibition detected by the disc diffusion test shows a strong bactericidal effect of the filters.
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