Synthesis and characterization of collagen/PLGA biodegradable skin scaffold fibers

Abstract

The aim of this study is to investigate the applicability of poly(lactic-co-glycolic acid) (PLGA)/collagen composite scaffold for skin tissue engineering. PLGA and collagen were dissolved in HFIP as a common solvent and fibrous scaffolds were prepared by electrospinning method. The scaffolds were characterized by scanning electron microscopy (SEM), FTIR spectroscopy, mercury porosimetry, tensile strength, biocompatibility assays and Biodegradation. Cytotoxicity and cell adhesion were tested for two cell line groups, human dermal fibroblast (HDF) and human keratinocyte (HaCat). SEM images showed appropriate cell adhesion to the scaffold for both cell lines. MTT assays indicated that the cell viability of HDF cells increased with time, but the number of HaCat cells decreased after 14 days. The ultimate tensile strength was suitable for skin substitute application, but its elongation at break was rather low. For successful clinical application of the PLGA/collagen scaffold, some properties especially mechanical strain needs to be improved.

Fabrication of a biomimetic spinal cord tissue construct with heterogenous mechanical properties using intrascaffold cell assembly

“…After sterilization, samples were submerged in sterile 2% v/v Type I collagen phosphate buffered saline (Sigma) solution for 30 min and left to air-dry in a sterile cell culture hood. As collagen is very hydrophilic, coating the PLGA scaffold with it improves cell-scaffold interaction to support cell adhesion and proliferation (Bi et al, 2018;Sadeghi-Avalshahr, Nokhasteh, Molavi, Khorsand-Ghayeni, & Mahdavi-Shahri, 2017). This coating technique was also used in our former study (Firouzian et al, 2019).…”

Electrospun PCL/mupirocin and chitosan/lidocaine hydrochloride multifunctional double layer nanofibrous scaffolds for wound dressing applications

“…21,23 Compared with synthetic polymers, natural polymers exhibit low immunogenicity and better biocompatibility and can mimic the native extracellular matrix (ECM) of biological tissue. 14,21,24 Widely used natural polymers for wound healing include chitosan, 1,16 collagen, 25,26 alginate, 27 gelatin, 28 chitin, 29 and silk fibroin. 30 However, wound dressings prepared from natural polymers are unable to retain their structural stability, 31 while those from synthetic polymers usually have good mechanical properties.…”

Digital Design and Automated Fabrication of Bespoke Collagen Microfiber Scaffolds

“…Despite the wide variety of scaffold technologies available to tissue engineers today, a compromise must often be made between the desirable cell adhesion and remodeling responses of natural polymers (like triple helical collagen), and the ease of production, mechanical robustness, and molecular durability of synthetic polymers, which have rapidly advanced novel fabrication technologies like 3D printing and electrospinning. In many cases, the solution takes the form of natural and synthetic copolymers and blends, such as polycaprolactone-collagen, collagen/polylactic acid, and collagen/poly(lactic-co-glyocolic acid), [57][58][59][60] to benefit from the mechanical integrity of synthetics and the cell-binding sites of collagen. The distinct advantages of incorporating wet-spun collagen microfibers into scaffolds presented herein are that it offers precise, customizable structural control and physiologically relevant mechanical robustness without any compromise in terms of cell adhesion site density, remodeling response, and immune response by simply using unadulterated collagen in both a structurally reinforcing fiber compartment and bulk hydrogel.…”