Synthesis and characterization of chitosan silver nanoparticle decorated with benzodioxane coupled piperazine as an effective anti-biofilm agent against MRSA: A validation of molecular docking and dynamics

Karthik, C.S.; Chethana, M.; Manukumar, H.M.; Ananda, A.P.; Sandeep, S.; Nagashree, S.; Mallesha, L.; Mallu, P.; Jayanth, H.S.; Dayananda, B. P.

doi:10.1016/j.ijbiomac.2021.03.119

Cited by 17 publications

(6 citation statements)

References 54 publications

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“…When the pH is lower than 10, the amino groups will be protonated and positively charged. The lipid layer on the surface of the biofilm is negatively charged and easy to electrostatically adsorb with positively charged chitosan particles [ 23 ]. As a pharmaceutical carrier, chitosan nanoparticles (CSNPs), with the advantages of slow or controlled drug release, can improve drug solubility and stability, thereby enhancing drug effects and reducing the side effects of drugs [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Antibiofilm Effect of Cinnamaldehyde-Chitosan Nanoparticles against the Biofilm of Staphylococcus aureus

Sheng

et al. 2022

Antibiotics

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Food contamination caused by food-spoilage bacteria and pathogenic bacteria seriously affects public health. Staphylococcus aureus is a typical foodborne pathogen which easily forms biofilm. Once biofilm is formed, it is difficult to remove. The use of nanotechnology for antibiofilm purposes is becoming more widespread because of its ability to increase the bioavailability and biosorption of many drugs. In this work, chitosan nanoparticles (CSNPs) were prepared by the ion–gel method with polyanionic sodium triphosphate (TPP). Cinnamaldehyde (CA) was loaded onto the CSNPs. The particle size, potential, morphology, encapsulation efficiency and in vitro release behavior of cinnamaldehyde–chitosan nanoparticles (CSNP-CAs) were studied, and the activity of CA against S. aureus biofilms was evaluated. The biofilm structure on the silicone surface was investigated by scanning electron microscopy (SEM). Confocal laser scanning microscopy (CLSM) was used to detect live/dead organisms within biofilms. The results showed that CSNP-CAs were dispersed in a circle with an average diameter of 298.1 nm and a zeta potential of +38.73 mV. The encapsulation efficiency of cinnamaldehyde (CA) reached 39.7%. In vitro release studies have shown that CA can be continuously released from the CSNPs. Compared with free drugs, CSNP-CAs have a higher efficacy in removing S. aureus biofilm, and the eradication rate of biofilm can reach 61%. The antibiofilm effects of CSNP-CAs are determined by their antibacterial properties. The minimum inhibitory concentration (MIC) of CA is 1.25 mg/mL; at this concentration the bacterial cell wall ruptures and the permeability of the cell membrane increases, which leads to leakage of the contents. At the same time, we verified that the MIC of CSNP-CAs is 2.5 mg/mL (drug concentration). The synergy between CA and CSNPs demonstrates the combinatorial application of a composite as an efficient novel therapeutic agent against antibiofilm. We can apply it in food preservation and other contexts, providing new ideas for food preservation.

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Section: Introductionmentioning

confidence: 99%

Antibiofilm Effect of Cinnamaldehyde-Chitosan Nanoparticles against the Biofilm of Staphylococcus aureus

Sheng

et al. 2022

Antibiotics

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“…95 The implants developed in this study exhibited strong antimicrobial activity against MRSA, prevention of biofilm formation, four times higher release of Ag + ions and two times larger ZOI. A distinctive anti-biofilm property of AgNPs against MRSA was confirmed by Karthik et al , 96 who studied benzodioxane coupled piperazine decorated chitosan silver nanoparticles (Bcp*C@AgNPs). These NPs were shown to prevent planktonic bacterial adherence and biofilm formation as was examined by the Crystal Violet staining method, SEM and CLSM, both quantitatively and qualitatively.…”

Section: Discussionmentioning

confidence: 82%

“…In this regard, silver NPs (AgNPs) have attracted significant interest from researchers due to their known properties such as high electrical and thermal conductivity, chemical stability, catalytic activity, as well as bactericidal, fungicidal, and antiviral potential. [93][94][95][96][97][98][99][100][101][102] In a recent study, AgNPs functionalized with jacalin (JAgNPs) were demonstrated to have an excellent S. aureus biofilm inhibitory effect at lower concentration than AgNPs.…”

Section: Nanotechnology Strategies For Biofilm Targeting and Eradicationmentioning

confidence: 99%

Nanotechnologies for control of pathogenic microbial biofilms

et al. 2022

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“…In one hand, a previous study involving the AMP C18N3/CARG by Jiang et al (2022) formed complexes of surfactants with hydrocarbons and the peptide, which led to β -lactamase inhibition and consequently, partial MRSA growth inhibition (∼50%) in the presence of antibiotics such as ampicillin. On the other hand, Karthik et al (2021) evaluated silver nanoparticles Bcp*C@AgNPs as an antibacterial agent against MRSA , obtaining only a 35% growth inhibition at a concentration of 320 μg/ml. Compared with these two separate studies discussing the potency of silver and an AMP as antibacterial agents, our formulation showed a significantly superior performance.…”

Section: Resultsmentioning

confidence: 99%

Formulation of a novel antibacterial topical treatment based on Magnetite-Buforin-II-silver nanobioconjugates

Muñoz

Jaramillo

Gantiva-Diaz

et al. 2022

Front. Bioeng. Biotechnol.

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Community acquired infections caused by Meticillin-resistant Staphylococcus aureus (MRSA) have become a growing concern due to its impact on the world public health. This microorganism is a commonly spreading pathogen associated predominantly with skin infections and connected to other more severe conditions (septic shock, and generalized infection). The lack of highly effective antibiotics and treatments to control skin infections with S. aureus has led to the search of novel therapies using alternative agents such as antimicrobial peptides (AMPs). In order to obtain a viable administration route to counteract superficial skin infections (impetigo, abscesses, furuncles, and cellulitis), a topical formulation based on Magnetite-Buforin-II-silver nanobioconjugates as active antibacterial agents was designed by their dispersion in O/W concentrated emulsions. The prepared topical characterization indicated that O/W emulsions were stable in time, the droplets size remained within the appropriate values (∼1 µm) and their rheological properties, such as pseudoplastic and shear-thinning behavior, remained unchanged for up to 3 months. Additionally, hemolysis and platelet aggregation tests were acceptable (i.e., 14.72 ± 2.62% and 8.06 ± 2.90%, respectively) in compliance with the ISO-10993 standard. Furthermore, the treatment reduced significantly (p < 0.0001) the growth of both clinical isolated MRSA and wild Type S. aureus strains as evidenced by the contact diffusion method. These results are important in the context of proposing new alternatives that allow manage effectively the threat posed by the antibiotic resistant bacterial strains, which jeopardize the lives of thousands of people every year.

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Synthesis and characterization of chitosan silver nanoparticle decorated with benzodioxane coupled piperazine as an effective anti-biofilm agent against MRSA: A validation of molecular docking and dynamics

Cited by 17 publications

References 54 publications

Antibiofilm Effect of Cinnamaldehyde-Chitosan Nanoparticles against the Biofilm of Staphylococcus aureus

Antibiofilm Effect of Cinnamaldehyde-Chitosan Nanoparticles against the Biofilm of Staphylococcus aureus

Nanotechnologies for control of pathogenic microbial biofilms

Formulation of a novel antibacterial topical treatment based on Magnetite-Buforin-II-silver nanobioconjugates

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