Synthesis and Characterization of Bicalutamide-Loaded Magnetic Nanoparticles as Anti-Tumor Drug Carriers

Abstract

This study examined the optical characteristics of bicalutamide-loaded magnetic/ethylene glycol composite nanoparticles (BMP), as well as their anti-cancer activity against cancer cells. The gamma-Fe2O3 magnetic nanoparticles (MNPs), approximately 20 nm in diameter, were prepared via a chemical co-precipitation method and coated with two surfactants to yield a water-based product. The characteristics of the particles were determined via X-ray diffraction (XRD), field emission scanning electron microscopy, and Raman spectrophotometry. The Raman spectra of the BMP showed peaks at 222, 283, 395, 520, 669 and 1316 cm(-1), with broadened band in comparison to the Raman spectra of the magnetic nanoparticles. The BMP absorbance evidenced a rapid increase, with a broad peak at 409 nm, thus reflecting a good loading of the bicalutamide onto the magnetic nanoparticles. The results of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that the MNPs were non-toxic against human brain cancer cells (SH-SY5Y), human cervical cancer cells (Hela), human liver cancer cells (HepG2), breast cancer cells (MCF-7), colon cancer cells (CaCO2) and human prostate cancers (Du 145, PC3) tested herein. In particular, BMPs were cytotoxic at 56% against DU145 cells, at 74.37% in SH-SY5Y cells, and at 58% in Hela cells. Our results demonstrated the biological applicability of BMP nanoparticles as anticancer agents and as agents for enhanced drug delivery against human prostate cancer cells. Our results indicated that the MNPs were biostable and that the BMP functioned effectively as drug delivery vehicles.

Cytotoxicity of nickel zinc ferrite nanoparticles on cancer cells of epithelial origin

“…4 In anticancer studies, magnetic nanoparticles are widely used in medical examinations, targeting, and treatment. 5 For detection purposes, incorporating magnetic nanoparticles into imaging modalities, such as magnetic resonance imaging (MRI), confers enhanced performance to cancer diagnosis. 6 In another advanced study, magnetic nanoparticles were utilized to detect tumors with diameters as small as 10 mm, which enabled medical doctors to discover cancers at early stages of malignancy.…”

Magnetic nanoformulations for prostate cancer

“…Therefore, magnetic nanoparticles represent a promising carrier for many other anticancer drug molecules such as 5-fluorouracil (5-FU) [22], doxorubicin (DOX) [37], flutamide (antagonist of the androgen receptor) [47], bicalutamide (antiandrogen) [42], and zoledronate (antiosteoclastic properties) [43]. In addition, lipid based magnetic nanoparticles are proposed extensively due to their drug depositing efficiency in hydrophobic layers and convenience to synthesize using seed-growth methods [15,48].…”

In Vivo Imaging of Transplanted Islets Labeled with a Novel Cationic Nanoparticle

“…However, this method is limited by the short isotope half-life and low spatial resolution. Magnetic resonance imaging (MRI) is an attractive potential tool for measuring the islet mass in vivo , because it is generally noninvasive, it can achieve relatively high spatial resolution, and it can use multiple mechanisms for contrast enhancement [6]–[8]. However, the electromagnetic properties of the islet and liver tissue do not differ considerably, and therefore, labeling with an MR contrast agent is necessary for their discrimination [9].…”