Synthesis and characterization of alginate nanocarrier encapsulating Artemisia ciniformis extract and evaluation of the cytotoxicity and apoptosis induction in AGS cell line

Rahimivand, Mahdieh; Tafvizi, Farzaneh; Noorbazargan, Hassan

doi:10.1016/j.ijbiomac.2020.05.006

Cited by 29 publications

(17 citation statements)

References 58 publications

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“…Based on this, it has been declared that the drug-loaded niosomes could advance the process of apoptosis in cancer cell lines via delivery of a high-level dose of drug molecules into cancer cells. This has different direct impacts on the expression levels of genes in the cells, and in turn, various ways for apoptosis could be activated (i.e., depending on drug and cell type), which could result in a high rate of apoptosis [ 16 , 67 ].…”

Section: Resultsmentioning

confidence: 99%

Super Magnetic Niosomal Nanocarrier as a New Approach for Treatment of Breast Cancer: A Case Study on SK-BR-3 and MDA-MB-231 Cell Lines

Jamshidifar

Yeganeh

Shayan

et al. 2021

IJMS

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In the present study, a magnetic niosomal nanocarrier for co-delivery of curcumin and letrozole into breast cancer cells has been designed. The magnetic NiCoFe2O4 core was coated by a thin layer of silica, followed by a niosomal structure, allowing us to load letrozole and curcumin into the silica layer and niosomal layer, respectively, and investigate their synergic effects on breast cancer cells. Furthermore, the nanocarriers demonstrated a pH-dependent release due to the niosomal structure at their outer layer, which is a promising behavior for cancer treatment. Additionally, cellular assays revealed that the nanocarriers had low cellular uptake in the case of non-tumorigenic cells (i.e., MCF-10A) and related high viability but high cellular uptake in cancer cell lines (i.e., MDA-MB-231 and SK-BR-3) and related low viability, which is evidenced in their high cytotoxicity against different breast cancer cell lines. The cytotoxicity of the letrozole/curcumin co-loaded nanocarrier is higher than that of the aqueous solutions of both drugs, indicating their enhanced cellular uptake in their encapsulated states. In particular, NiCoFe2O4@L-Silica-L@C-Niosome showed the highest cytotoxicity effects on MDA-MB-231 and SK-BR-3 breast cancer cells. The observed cytotoxicity was due to regulation of the expression levels of the studied genes in breast cancer cells, where downregulation was observed for the Bcl-2, MMP 2, MMP 9, cyclin D, and cyclin E genes while upregulation of the expression of the Bax, caspase-3, and caspase-9 genes was observed. The flow cytometry results also revealed that NiCoFe2O4@L-Silica-L@C-Niosome enhanced the apoptosis rate in both MDA-MB-231 and SK-BR-3 cells compared to the control samples. The findings of our research show the potential of designing magnetic niosomal formulations for simultaneous targeted delivery of both hydrophobic and hydrophilic drugs into cancer cells in order to enhance their synergic chemotherapeutic effects. These results could open new avenues into the future of nanomedicine and the development of theranostic agents.

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Section: Resultsmentioning

confidence: 99%

Super Magnetic Niosomal Nanocarrier as a New Approach for Treatment of Breast Cancer: A Case Study on SK-BR-3 and MDA-MB-231 Cell Lines

Jamshidifar

Yeganeh

Shayan

et al. 2021

IJMS

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“…Anisomeles is a genus of herb of the family Lamiaceae. The previous reports have indicated that the same cytotoxic properties in the induction of apoptosis in AGS cells of nanoparticles prepared from the Artemisia turcomanica and Artemisia ciniformis extracts [ 18 , 19 ]. However, there have been no studies to date developing a simple and efficient purification method for the active compound of Ova of the plants and on the cytotoxic activities on gastric cancer cells at the same time.…”

Section: Discussionmentioning

confidence: 97%

Innovative Purification Method of Ovatodiolide from Anisomeles indica to Induce Apoptosis in Human Gastric Cancer Cells

et al. 2022

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Ovatodiolide (Ova), found in the plant Anisomeles indica (AI), has been reported to have an anti-proliferation effect in various cancer cells. However, little information is available regarding the anti-cancer effect of Ova in human gastric cancer cells. In this study, we investigated the inhibitory effects and the mechanisms of action responsible for these effects on human AGS cell lines from a newly developed purification technique for Ova from AI extract. Extract obtained at the optimum condition of 95% ethanol extraction of AI was sequentially partitioned by using different polarity solvents. Enriched content of Ova (35.9% purity) from the n-hexane fraction was then applied to the purification by using centrifugal partition chromatography (CPC) in a two-phase solvent system consisting of n-hexane:ethyl acetate:methanol:water (1.0:1.0:1.0:1.0, v/v/v/v) to reach purity over >95.0%. In evaluation of the anti-proliferation effect on AGS cells, Ova induced cell apoptosis with IC50 values of 13.02 and 6.18 μM at 24 and 48 h, respectively, and arrested the cells at the G2/M phase. Quantification of Bax/Bcl2 mRNA expressions using qPCR showed a 2.5-fold increase in the Ova (5 μM)-treated cells at 48 h than in the control group. Specific protein expression data warrant further research to further confirm the proposed Ova-induced apoptotic pathway in AGS cells.

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“…Swathi and George, using cerium oxide (CeO 2 ) nanoparticles loaded with Nelumbo nucifera flower extract, reported an in-vitro cytotoxic effect against human colon cancer cell line HCT 116, with an IC 50 of 41.6 mg/mL 77 . Rahimivand and researchers., determined Artemisia ciniformis extract cytotoxic activity encapsulated in sodium alginate nanogel on the gastric cancer cell line AGS; results showed apoptosis induction in a dose-time-dependent manner with an IC 50 of 21.5, 18.5, and 15.2 μg/mL, after 24, 48, and 72 h. respectively compared to extract alone 61.9, 40.07, and 28.7 μg/mL after 24, 48, and 72 h respectively 78 . On the other hand, Olea and researchers, analyzed ethyl acetate and ethanolic Leptocarphari vularis extracts incorporated in polymeric micelles formed from Pluronic F127 against the cancer cell lines HT-29 (colon cancer) and PC-3 (prostate cancer).…”

Section: Gastrointestinal Infectionmentioning

confidence: 97%

Untitled

2022

IJPSR

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An extensive range of studies have shown plant extracts and their phyto-constituents efficacy, proposing them as a promising alternative for treatment or as adjuvants in human diseases such in skin, gastrointestinal, systemic and dental conditions, due to various active components presence, ease of access and reduced side effects. Indeed, depending on solvent used to obtain the extract, its active molecules may vary, including flavonoids, tannins, alkaloids, terpenes, sesquiterpenes, sterols, among others. Extract content (crude/partition/fraction) has pharmacological advantages over isolated compounds, due to pleiotropic activity and synergistic effect between components. Due to factors such as low bioavailability, solubility, and stability, it is difficult to translate its in-vitro and in-vivo effects into clinical picture, besides that pharmacological effects full potential cannot be exploited. However, in recent decades, extraordinary advances have been made in new drug delivery systems for the encapsulation of active plant metabolites, thus helping to improve different medicinal plant-based therapies and product efficacy. The current review highlights the importance of nanocarriers loaded with plant extracts as an alternative for dental, dermatological, gastrointestinal, and systemic diseases.

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Synthesis and characterization of alginate nanocarrier encapsulating Artemisia ciniformis extract and evaluation of the cytotoxicity and apoptosis induction in AGS cell line

Cited by 29 publications

References 58 publications

Super Magnetic Niosomal Nanocarrier as a New Approach for Treatment of Breast Cancer: A Case Study on SK-BR-3 and MDA-MB-231 Cell Lines

Super Magnetic Niosomal Nanocarrier as a New Approach for Treatment of Breast Cancer: A Case Study on SK-BR-3 and MDA-MB-231 Cell Lines

Innovative Purification Method of Ovatodiolide from Anisomeles indica to Induce Apoptosis in Human Gastric Cancer Cells

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