Synthesis and characteristics of NH2-functionalized polymer films to align and immobilize DNA molecules

Abstract

We developed a method to use NH2-functionalized polymer films to align and immobilize DNA molecules on a Si substrate. The plasma-polymerized cyclohexane film was deposited on the Si substrate according to the radio frequency plasma-enhanced chemical vapor deposition method using a single molecular precursor, and it was then treated by the dielectric barrier discharge method in a nitrogen environment under atmospheric pressure. Changes in the chemistry of the surface functional groups were studied using X-ray photoelectron spectroscopy and Fourier transformed infrared spectroscopy. The wettability of the surfaces was examined using dynamic contact angle measurements, and the surface morphology was evaluated using atomic force microscopy.We utilized a tilting method to align λ-DNA molecules that were immobilized by the electrostatic interaction between the amine groups in NH2-functionalized polymer films and the phosphate groups in the DNA. The DNA was treated with positively charged gold nanoparticles to make a conductive nanowire that uses the DNA as a template. We observed that the NH2-functionalized polymer film was useful for aligning and immobilizing the DNA, and thus the DNA-templated nanowires.

VUV treatment combined with mechanical strain of stretchable polymer foils resulting in cell alignment

“…is found for both irradiated samples. This is possibly an additional N C O function, similar as to the one found in the structure formula of poly(urea) or to the feature described by Kim et al [32], and is in agreement with a broadening of the corresponding N1s high-resolution spectra observed by us on the treated surfaces. In summary, the XPS results indicate a relative increase of N-containing groups after the VUV irradiation in the reactive N 2 and more pronounced in NH 3 atmosphere.…”

Shape memory effect on the formation of oxazoline and triazine rings of BCC/DGEBA copolymer

“…The incorporation of 50 wt% of BCC that is beyond the optimum percentage level a new absorption peak corresponding to the triazine ring was observed at 1357 cm -1 due to the self-polymerization of cyanate ester and further the peak intensity increases with an increase in the weight percentage of BCC cyanate ester 18 . Subsequently, the third peak at 286.1 eV represents N=C-O bonds of the oxazoline ring indicating the successful epoxy and cyanate ester co-reaction and the final peak at 288.1 eV confirm the C-C=N bond formation 26 . 2) was used to confirm the various types of bonds present in the cured copolymer.…”

Molecular Threading: Mechanical Extraction, Stretching and Placement of DNA Molecules from a Liquid-Air Interface

“…We investigated local roughness and waviness of threads by atomic-resolution TEM, and compared our findings with combing based on several results from the literature [6], [19], [20], [32], [33], [34], [35], [36], [37], [38], [39], [40] (see Figure 5 ). Threads are extremely straight both locally (<100 nm length) and over larger subunit lengths (>100 nm length).…”