Synthesis and characterisation of arsenic nanoparticles and its interaction with DNA and cytotoxic potential on breast cancer cells

Subastri, Ariraman; Viswanathan, Arun; Sharma, Preeti; Babu, E. Preedia; Arumugam, Suyavaran; Nithyananthan, Subramaniyam; Alshammari, Ghedeir M.; Balakrishnan, Arun; Dharuman, Venkataraman; Thirunavukkarasu, Chinnasamy

doi:10.1016/j.cbi.2017.12.025

Cited by 33 publications

(22 citation statements)

References 58 publications

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“…Cancer is one of the major health problems worldwide [1] with an estimation of seven million new cases and 13 million deaths by 2030 according to National Cancer Institute of USA.…”

Section: Introductionmentioning

confidence: 99%

β-Cyclodextrin complex improves the bioavailability and antitumor potential of cirsiliol, a flavone isolated from Leonotis nepetifolia (Lamiaceae)

Oliveira

Silva

Viana

et al. 2019

Heliyon

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Cirsiliol is a flavone found in many Lamiaceae species with high cytotoxic activity against tumor cell lines. Although cirsiliol is being used in cancer therapy, its pharmacological potential is limited by its low solubility and bioavailability. In this paper, a cirsiliol-β-cyclodextrin inclusion complex was developed in order to increase its solubility and bioavailability. The formation of inclusion complex was proved by scanning electron microscopy, Fourier-transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR) and solubility increment was verified through the ultraviolet-visible (UV-Vis) method. The cytotoxic effect against tumor cells (PC3, HCT-116 and HL-60 human cell lines, and S-180 murine cell line) and the antitumor activity in mice bearing sarcoma S-180 were also investigated. The inclusion complex was obtained with 71.45% of total recovery and solubility 2.1 times higher compared to the compound in its free form. This increment in solubility was responsible by a tumor growth inhibition potentiation (1.5 times greater compared to compound in its free form). In addition, this study showed that cirsiliol and its inclusion complex in β-cyclodextrin have strong antitumor potential at low doses without promoting side effects commonly observed for conventional drugs as doxorubicin.

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“…Cancer is one of the major health problems worldwide [1] with an estimation of seven million new cases and 13 million deaths by 2030 according to National Cancer Institute of USA.…”

Section: Introductionmentioning

confidence: 99%

β-Cyclodextrin complex improves the bioavailability and antitumor potential of cirsiliol, a flavone isolated from Leonotis nepetifolia (Lamiaceae)

Oliveira

Silva

Viana

et al. 2019

Heliyon

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“…30 Arsenic nanoparticles were safer than arsenic trioxide with equivalent efficacy against breast cancer cell lines. 31 It seems as if arsenic acts as a double-edged sword and, depending on the biological states of cell and arsenic concentration, diverse effects would be displayed. Therefore, we purposely selected arsenic and also considered a way to reduce its size (nanoparticles) to see if there would be any modification in its toxico-dynamic or mechanism of action.…”

Section: Discussionmentioning

confidence: 99%

Subcellular Organelle Toxicity Caused by Arsenic Nanoparticles in Isolated Rat Hepatocytes

Jahangirnejad

Goudarzi

Kalantari‬

et al. 2020

Int J Occup Environ Med

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Background: Arsenic, an environmental pollutant, is a carcinogenic metalloid and also an anticancer agent. Objective: To evaluate the toxicity of arsenic nanoparticles in rat hepatocytes. Methods: Freshly isolated rat hepatocytes were exposed to 0, 20, 40, and 100 µM of arsenic nanoparticles and its bulk counterpart. Their viability, reactive oxygen species level, glutathione depletion, mitochondrial and lysosomal damage, and apoptosis were evaluated. Results: By all concentrations, lysosomal damage and apoptosis were clearly evident in hepatocytes exposed to arsenic nanoparticles. Evaluation of mitochondria and lysosomes revealed that lysosomes were highly damaged. Conclusion: Exposure to arsenic nanoparticles causes apoptosis and organelle impairment. The nanoparticles have potentially higher toxicity than the bulk arsenic. Lysosomes are highly affected. It seems that, instead of mitochondria, lysosomes are the first target organelles involved in the toxicity induced by arsenic nanoparticles.

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“…To distinguish apoptotic and necrotic cells, AO/EB staining procedure was adopted as explained by Subastri et al [ 23 ]. In brief, MCF-7 cells were grown in 24-well cell culture plates (10 5 cells/well).…”

Section: Methodsmentioning

confidence: 99%

“…To distinguish apoptotic and necrotic cells, AO/EB staining procedure was adopted as explained by Subastri et al [23].…”

Section: Acridine Orange/ethidium Bromide (Ao/eb) Stainingmentioning

confidence: 99%

Cucurbita ficifolia Fruit Extract Induces Tp53/Caspase-Mediated Apoptosis in MCF-7 Breast Cancer Cells

Alshammari

Balakrishnan

Alshatwi

et al. 2020

BioMed Research International

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The second most biggest cancer worldwide is breast cancer. There is an increasing need for safer, effective, and affordable drug candidates from natural sources to treat breast cancer. In the present investigation, the anticancer effect of Cucurbita ficifolia Bouché (C. ficifolia) fruit extract was tested on the human breast cancer cells such as MCF-7. The cells were exposed with different doses of C. ficifolia, for the assessment of IC50 concentrations on the MCF-7 cell lines for 24 hs. The effect of C. ficifolia fruit extract on morphological and apoptotic changes were evaluated by specific fluorescence staining techniques and real-time PCR in a time-dependent manner for 24 hs and 48 hs. The IC50 value for C. ficifolia fruit extract was found to be 90 μg/mL. Morphological alteration and apoptotic distinctiveness aspect like chromatin condensation and nuclear fragmentation were noticed in C. ficifolia extract exposed breast cancer cells. Further, we observed that C. ficifolia extract-induced programmed cell death in the MCF-7 cells were mediated with the elevated expression of the tumor suppressor gene such as p53 and apoptotic markers such as caspase-8, caspase-9, caspase-3, fatty acid synthase (FAS), Fas-associated protein with death domain (FADD), Bcl-2 homologous antagonist/killer (BAK), and Bcl-2-associated X protein (BAX). These observations established that C. ficifolia significantly concealed the cell division and provoked p53/caspase-mediated programmed cell death. Further, we noticed that this cell death in MCF-7 cells is concentration and time dependent. As evaluated through the comet assay, C. ficifolia induced DNA damage; further upon increasing the duration of the treatment, the DNA damage was higher than before. Thus, our study concludes that C. ficifolia could serve as an effective anticancer agent through vital gene modulation.

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Synthesis and characterisation of arsenic nanoparticles and its interaction with DNA and cytotoxic potential on breast cancer cells

Cited by 33 publications

References 58 publications

β-Cyclodextrin complex improves the bioavailability and antitumor potential of cirsiliol, a flavone isolated from Leonotis nepetifolia (Lamiaceae)

β-Cyclodextrin complex improves the bioavailability and antitumor potential of cirsiliol, a flavone isolated from Leonotis nepetifolia (Lamiaceae)

Subcellular Organelle Toxicity Caused by Arsenic Nanoparticles in Isolated Rat Hepatocytes

Cucurbita ficifolia Fruit Extract Induces Tp53/Caspase-Mediated Apoptosis in MCF-7 Breast Cancer Cells

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