Synthesis and application of superabsorbent polymer microspheres for rapid concentration and quantification of microbial pathogens in ambient water

Wu, Xunyi; Huang, Xiumin; Zhu, Yuqing; Li, Jing; Hoffmann, Michael R.

doi:10.1016/j.seppur.2020.116540

Cited by 29 publications

(28 citation statements)

References 42 publications

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“…Consecuently, to include sample preconcentration steps prior to analysis is necessary [23]. Different preconcentration methods based on immunomagnetic separation (IMS) systems [24][25][26][27], centrifugation [28,29] and membrane filtration have been coupled to sensing methods. Nevertheless, membrane filtration is considered the first choice for large sample volumes (≥10 mL) [30,31].…”

Section: Introductionmentioning

confidence: 99%

Rapid Detection of Legionella pneumophila in Drinking Water, Based on Filter Immunoassay and Chronoamperometric Measurement

et al. 2020

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Legionella is a pathogenic bacterium, ubiquitous in freshwater environments and able to colonise man-made water systems from which it can be transmitted to humans during outbreaks. The prevention of such outbreaks requires a fast, low cost, automated and often portable detection system. In this work, we present a combination of sample concentration, immunoassay detection, and measurement by chronoamperometry. A nitrocellulose microfiltration membrane is used as support for both the water sample concentration and the Legionella immunodetection. The horseradish peroxidase enzymatic label of the antibodies permits using the redox substrate 3,3′,5,5′-Tetramethylbenzidine to generate current changes proportional to the bacterial concentration present in drinking water. Carbon screen-printed electrodes are employed in the chronoamperometric measurements. Our system reduces the detection time: from the 10 days required by the conventional culture-based methods, to 2–3 h, which could be crucial to avoid outbreaks. Additionally, the system shows a linear response (R2 value of 0.99), being able to detect a range of Legionella concentrations between 101 and 104 cfu·mL−1 with a detection limit (LoD) of 4 cfu·mL−1.

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Section: Introductionmentioning

confidence: 99%

Rapid Detection of Legionella pneumophila in Drinking Water, Based on Filter Immunoassay and Chronoamperometric Measurement

et al. 2020

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“…3 ). The PCC was pre-filled with dry SAP beads, which were designed to absorb water molecules through osmosis and to reject bacteria from entering through size exclusion and negative surface charge ( Wu et al, 2020 ). Various CD rotation speed and duration were tested in experimental trials to drive the concentrated sample up the sides of the walls and through the cup outlet ( Fig.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, the microfluidic CD also provides a platform to integrate sample concentration as a pretreatment, which is often a critical step for water sample analysis. We report the development of a pathogen concentration cup (PCC) filled with super absorbent polymer (SAP) beads for passive concentration of bacteria from water ( Wu et al, 2020 ; Xie et al, 2016 ). The outcomes of this research demonstrated the proof-of-concept of a PPAS for water quality monitoring.…”

Section: Introductionmentioning

confidence: 99%

Development of a proof-of-concept microfluidic portable pathogen analysis system for water quality monitoring

Gowda

Kido

et al. 2022

Science of The Total Environment

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“…Early work investigating its toxicity in mammals already suggested a link between itaconate and the citric acid cycle. [3][4] For several decades, however, itaconate was of interest mostly as a building block in the synthesis of renewable polymers with applications ranging from superabsorbent microspheres 5 to trace metal detection, 6 and pH-sensitive drug delivery. 7 This led to the exploration, the bioengineering, and even the discovery of different itaconate-producing organisms such as other Aspergillus species, Ustilago species, and Escherichia coli, thereby improving its economic viability as a polymer starting material.…”

Section: Introductionmentioning

confidence: 99%

Itaconate: an antimicrobial metabolite of macrophages

Duncan

Auclair

2022

Can. J. Chem.

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Itaconate is a conjugated 1,4-dicarboxylate produced by macrophages. This small molecule has recently received increasing attention due to its role in modulating the immune response of macrophages upon exposure to pathogens. Itaconate has also been proposed to play an antimicrobial function; however, this has not been explored as intensively. Consistent with the latter, itaconate is known to show antibacterial activity in vitro and was reported to inhibit isocitrate lyase, an enzyme required for survival of bacterial pathogens in mammalian systems. Recent studies have revealed bacterial growth inhibition under biologically relevant conditions. In addition, an antimicrobial role for itaconate is substantiated by the high concentration of itaconate found in bacteria-containing vacuoles, and by the production of itaconate-degrading enzymes in pathogens such as Salmonella enterica ser. Typhimurium, Pseudomonas aeruginosa, and Yersinia pestis. This review describes the current state of literature in understanding the role of itaconate as an antimicrobial agent in host-pathogen interactions.

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Synthesis and application of superabsorbent polymer microspheres for rapid concentration and quantification of microbial pathogens in ambient water

Cited by 29 publications

References 42 publications

Rapid Detection of Legionella pneumophila in Drinking Water, Based on Filter Immunoassay and Chronoamperometric Measurement

Rapid Detection of Legionella pneumophila in Drinking Water, Based on Filter Immunoassay and Chronoamperometric Measurement

Development of a proof-of-concept microfluidic portable pathogen analysis system for water quality monitoring

Itaconate: an antimicrobial metabolite of macrophages

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